IHC Solutions

High Sensitivity, Low Background
Immunohistochemistry Products

High Sensitivity. Low Background. True Results.

For decades, immunohistochemistry (IHC) has been an invaluable tool for the detection, localization, and quantification of antigens in preserved tissue for research and diagnostic purposes. Continuing our position as a leader in detection technologies, Enzo Life Sciences offers a complete set of tools to help improve the quality and efficiency of your IHC process from start to finish. Our high-sensitivity, low background reagents, combined with the market’s most extensive palette of unique chromogens provide the flexibility needed for simple or complex protocols. Our convenient kits, an extensive catalog of IHC validated antibodies, and high definition chromogens enable accurate, reproducible multiplex detection of two or more antigens in the same sample, increasing efficiency and preserving precious tissue samples.

Detect with Confidence

Enzo’s POLYVIEW® PLUS Detection System Eliminates False Positive Results Observed with Common Commercially Available Kits

Comparative analysis of Enzo with a leading market competitor, using samples with clinical relevance, demonstrates elimination of false positive results

Breast cancer tissue negative for HER2/Neu
As expected, tissue stained with POLYVIEW® PLUS HRP Detection System is negative for HER2/Neu; the competitor HRP detection gave a false positive result
HER2/Neu negative, confirmed by FISH analysis

Competitor HRP Detection
(HER2/Neu is false positive)

Enzo’s POLYVIEW® PLUS HRP Detection
(HER2/Neu is negative)

Cervical cancer tissue negative for p16
As expected, tissue stained with POLYVIEW® PLUS HRP Detection System is negative for p16; the competitor HRP detection gave a false positive result

Competitor HRP Detection
(p16 is false positive)

Enzo’s POLYVIEW® PLUS HRP Detection
(p16 is negative)

Customer Testimonials

"The POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) kit worked very well; there was no background and the protocol is faster than many other IHC kits available commercially. I would surely recommend it. I was really happy with the POLYVIEW® PLUS HRP-DAB (Anti-Mouse) kit too."
– Columbia University

"We tested Enzo’s POLYVIEW® IHC detection reagents and HIGHDEF® chromogens on our immunohistochemical autostainers against similar available detection reagents and chromogens. We found that Enzo’s detection reagents and chromogens matched or exceeded what we currently used, allowing for increased sensitivity and specificity when used against both human and murine tissue."
– The Beatson Institute/p>

Clear and Consistent Results

POLYVIEW® PLUS Nanopolymer Peroxidase Detection Solutions

Complete kits with high-sensitivity and low background deliver clear results

Complete ready-to-use staining kits and reagents for IHC and ISH
High sensitivity POLYVIEW® PLUS reagents’ biotin-free nanopolymer detection circumvents endogenous background while providing specifi c antigen localization
HIGHDEF® DAB (Brown) HRP provides high intensity color development to deliver sharp, crisp staining
Compatible with manual and automated platforms

Clear, consistent results eliminate ambiguity

Formalin-fixed paraffin-embedded cervical tissue stained using POLYVIEW® PLUS HRP-DAB (Anti-Rabbit) Kit to detect Ki-67 on an autostainer (Leica Bond Rx.)

SAVIEW® PLUS Streptavidin Detection Solutions

Streptavidin-based detection with increased sensitivity and lower background

Reduced carbohydrate and biotin background
Optimized ready-to-use reagents for IHC and ISH detection
Compatible with both manual and automated platforms

Strong signal and low background provides clear results

Formalin-fixed paraffin-embedded cervical tissue stained using SAVIEW® PLUS HRP Reagent to detect Ki-67 on an autostainer (Leica Bond Rx.)

Multiplex Detection Kits

Superior MULTIVIEW® PLUS IHC kits for effi cient detection of multiple antigens in a single tissue sample

Biotin-free nanopolymer-based system for superior multiplex antigen detection
Complete kit includes HIGHDEF® chromogens for sensitive, crisp 2-color development
Preserves precious tissue while reducing reagent and labor costs
Expandable with additional chromogens (sold separately)

Superior detection of multiple antigens

Formalin-fixed paraffin-embedded human tonsil double stained using MULTIVIEW® PLUS (mouse-HRP/rabbit-AP) IHC kit. Primary rabbit anti-Ki-67 antibody was visualized with HIGHDEF® red IHC chromogen (AP), while primary mouse anti-HMW CK antibody was visualized with HIGHDEF® yellow IHC chromogen (HRP), producing a distinct golden yellow color contrasting with the bright red