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WESTERNVIEW™ Detection Kit (Anti-Rabbit)

Chromogenic  Western Blot kit with increased signal and sensitivity
 
ENZ-KIT183-0001 1 Kit 349.00 USD
 
  • More sensitive detection - able to detect lower concentrations of protein
  • Easy to use - all reagents are ready-to-use or ready-to-dilute, saving precious time
  • Amenable to multiplex analysis - determine source of protein bands with differentiated colors
  • Easily adaptable - works with a wide range of mouse-derived proteins
The WESTERNVIEW™ Detection Kit (Anti-Rabbit) can be used to identify primary antibodies on a Western Blot using immunodetection. The kit contains a complete reagent set, with optimized ready-to-use or ready-to-dilute reagents.
WESTERNVIEW Detection Kit (Anti-Rabbit) WB Caspase-7
Caspase-7, cleaved and uncleaved, run on the WESTERNVIEW™ Detection Kit and a competitor's kit.
WESTERNVIEW Detection Kit (Anti-Rabbit) WB Jurkat
Western Blot of Jurkat cells using WESTERNVIEW™ Detection Kit
WESTERNVIEW Detection Kit (Anti-Rabbit) WB Linear dilution
Linear dilution of protein (µg) run in the WESTERNVIEW™ Detection Kit (Anti-Rabbit) and the competitor's kit.
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WESTERNVIEW Detection Kit (Anti-Rabbit) WB Caspase-7 WESTERNVIEW Detection Kit (Anti-Rabbit) WB Jurkat WESTERNVIEW Detection Kit (Anti-Rabbit) WB Linear dilution

Product Specification

Assay Time:2 hours
 
Applications:WB, Colorimetric detection
 
Species reactivity:Rabbit
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Contents:
  1. Antibody Diluent, 4 x 90 mL
    Diluent solution for dilution of primary and secondary antibodies.
  2. NBT/BCIP, Ready-to-Use, 100 mL
    Ready-made solution for chromogenic blotting.
  3. Wash Buffer (10X), 2 x 100 mL
    10X Wash Buffer for washing membrane.
  4. Secondary Antibody (AP Anti-Rabbit), 4.5 mL
    Anti-Rabbit secondary antibody for detection of rabbit-based primary antibodies in Western Blot.
 
Scientific Background:Western Blotting is a common lab technique used to detect proteins in a sample of tissue homogenate or extract. Due to its flexibility, it can be used for a wide range of applications within molecular biology and biochemistry, including detection of post-translational modifications and verification of protein cloning. Using the size and color intensity of the protein band, a semi-quantitative estimation of protein can be  derived.
 
Protocol:WESTERNVIEW™ Detection Kit Basic Protocol
  1. Incubate with primary antibody, place the membrane in a plastic tray and wash.
  2. Incubate with secondary antibody provided in kit.
  3. Incubate with NBT/BCIP provided for 10 seconds to 2.5 minutes.
  4. Wash in distilled water and scan the membrane.
For a more detailed protocol, please reference the kit insert.
 
Regulatory Status:RUO - Research Use Only
 

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