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WESTERNVIEW™ Dual Detection Kit (Anti-Mouse/Anti-Rabbit)

Chromogenic Western Blot kit with increased signal and sensitivity
 
ENZ-KIT163-0001 1 Kit 349.00 USD
 
  • More sensitive detection - able to detect lower concentrations of protein
  • Easy to use - all reagents are ready-to-use or ready-to-dilute, saving precious time
  • Amenable to multiplex analysis - determine source of protein bands with differentiated colors
  • Easily adaptable - works with a wide range of mouse-derived proteins
The WESTERNVIEW™ Dual Detection Kit (Anti-Mouse/Anti-Rabbit) can be used to identify primary antibodies on a Western Blot using immunodetection. The kit contains a complete reagent set, with optimized ready-to-use or ready-to-dilute reagents.
WESTERNVIEW Dual Detection Kit - PANC-1
PANC-1 cells treated with etoposide. The band at 50 kDa is clearly revealed to be tubulin, while the smaller bands are known to be cleaved caspase.
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WESTERNVIEW Dual Detection Kit - PANC-1

Product Specification

Assay Time:2 hours
 
Applications:WB
 
Species reactivity:Mouse
Rabbit
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:+4°C
 
Contents:
  1. Antibody Diluent, 4 x 90 mL
    Diluent solution for dilution of primary and secondary antibodies.
  2. NBT/BCIP, Ready-to-Use, 100 mL
    Ready-made solution for chromogenic blotting to be used when developing anti-rabbit secondary.
  3. DAB Chromogen (25X), 4 mL
    Concentrated chromogen for chromogenic blotting to be used when developing anti-mouse secondary.
  4. DAB Substrate Buffer, 100 mL
    Buffer used to dilute DAB Chromogen (25X).
  5. SignaSure® Buffer Salts, 2 packets
    Contents used to make SignaSure® Wash Buffer.
  6. Tween®-20, 2 mL
    Detergent for SignaSure® Wash Buffer.
  7. Secondary Antibody (HRP Anti-Mouse), 2 mL
    Anti-Mouse secondary antibody for detection of mouse-based primary antibodies in Western Blots. Use DAB Substrate Buffer and Chromogen to develop.
  8. Secondary Antibody (AP Anti-Rabbit), 2 mL
    Anti-Rabbit secondary antibody for detection of rabbit-based primary antibodies in Western Blots. Use NBT/BCIP solution to develop.
 
Scientific Background:Western Blotting is a common lab technique used to detect proteins in a sample of tissue homogenate or extract. Due to its flexibility, it can be used for a wide range of applications within molecular biology and biochemistry, including detection of post-translational modifications and verification of protein cloning. Using the size and color intensity of the protein band, a semi-quantitative estimation of protein can be  derived.
 
Regulatory Status:RUO - Research Use Only
 

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