| Alternative Name: | OPN, SPP1, Secreted phosphoprotein 1, Bone sialoprotein 1 |
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| Clone: | 53 |
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| Host: | Mouse |
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| Isotype: | IgG2a |
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| Immunogen: | Recombinant human Osteopontin. |
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| UniProt ID: | P10451 |
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| GenBank ID: | U20758 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Applications: | ELISA, IHC (FS), IHC (PS), WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2,containing 0.1% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to provide prognostic information in breast, prostate, colon, and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies. |
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| Regulatory Status: | RUO - Research Use Only |
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Western Blot analysis of ENZ-ABS753: Lane 1: MW Marker, Lane 2: HeLa lysate, Lane 3: MCF-7 lysate, Lane 4: A431 lysate, Lane 5: HEK-293 lysate, Lane 6: Caki-2 lysate.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained with Osteopontin monoclonal antibody (53), (Prod. No. ENZ-ABS753) at 10µg/ml.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained with Osteopontin monoclonal antibody (53), (Prod. No. ENZ-ABS753) at 10µg/ml.
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained with Osteopontin monoclonal antibody (53), (Prod. No. ENZ-ABS753) at 10µg/ml.
