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αB-Crystallin monoclonal antibody (1B6.1-3G4)

 
ENZ-ABS706-0200 200 µg 656.00 USD
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Product Details

Alternative Name:CRYAB, HSPB5
 
Clone:1B6.1-3G4
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Bovine αB-crystallin.
 
UniProt ID:P02510
 
Source:Purified from hybridoma tissue culture supernatant.
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken
 
Applications:ICC, IF, IHC (PS), WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (paraffin sections, 10µg/ml)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~20kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles. 
 
Shipping:Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Alpha-crystallins, which are part of the small Heat shock family members, are major water-soluble proteins present in the lens of the mammalian eye. Phosphorylation of serine residues which occurs during development and in response to stress, is intimately linked with its function. Chaperone activity requires, and is modulated by, oligomerization and is limited to binding unfolded intermediates to prevent irreversible aggregation.
 
Regulatory Status:RUO - Research Use Only
 
αB-Crystallin monoclonal antibody (1B6.1-3G4) Western blot
Western Blot analysis of αB-Crystallin mAb (1B6.1-3G4) (Prod. No. ENZ-ABS706): Lane 1: MW Marker, Lane 2: α-Crystallin (bovine), (native) (Prod. No. ADI-SPP-225), Lane 3: αB-Crystallin (bovine), (native) (Prod. No. ADI-SPP-227).
αB-Crystallin monoclonal antibody (1B6.1-3G4) IHC
Immunohistochemistry analysis of human heart tissue with alpha B-Crystallin, mAb (1B6.1-3G4), (Prod. No. ENZ-ABS706), at 20µg/ml.
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αB-Crystallin monoclonal antibody (1B6.1-3G4) Western blot αB-Crystallin monoclonal antibody (1B6.1-3G4) IHC

Product Literature References

The small heat shock protein αB-Crystallin protects versus withaferin A-induced apoptosis and confers a more metastatic phenotype in cisplatin-resistant ovarian cancer cells: M.M. Carmichael, et al.; PLoS One 18, e0281009 (2023), Abstract;

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