| Alternative Name: | Lymphocyte activation gene-3, FDC protein, CD223 |
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| Clone: | 17B4 |
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| Host: | Mouse |
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| Isotype: | IgG1κ |
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| Immunogen: | Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3). |
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| UniProt ID: | P18627 |
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| GenBank ID: | NM_002286 |
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| Source: | Purified from hybridoma tissue culture supernatant. |
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| Species reactivity: | Human
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| Specificity: | Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of LAG-3. |
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| Applications: | Flow Cytometry, ICC
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| Recommended Dilutions/Conditions: | Flow Cytometry (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50mM Tris, 1% BSA and 0.02% sodium azide. |
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| Handling: | Do not freeze. Protect from light. |
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| Shipping: | Blue Ice Not Frozen |
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| Short Term Storage: | +4°C |
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| Long Term Storage: | +4°C |
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| Scientific Background: | The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses. |
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| Regulatory Status: | RUO - Research Use Only |
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Flow cytometry analysis of 106 HDLM-2 cells stained using LAG-3 mAb (L4-PL33) (ATTO 647N conjugate), (Prod. No. ENZ-ABS677TS), at a concentration of 10µg/ml, side-by-side comparison to LAG-3 mAb (17B4) (ATTO 647N conjugate), (Prod. No. ALX-804-806TS).
Flow cytometry analysis of 106 HDLM-2 cells stained using LAG-3 mAb (L4-PL33) (ATTO 647N conjugate), (Prod. No. ENZ-ABS677TS), at a concentration of 10µg/ml, side-by-side comparison to LAG-3 mAb (17B4) (ATTO 647N conjugate), (Prod. No. ALX-804-806TS).
