| ALX-804-806TD-T100 | 100 tests | ATTO 488 | Inquire for pricing | ||
| Do you need bulk/larger quantities? | |||||
| Alternative Name: | Lymphocyte activation gene-3, FDC protein, CD223 |
| Clone: | 17B4 |
| Host: | Mouse |
| Isotype: | IgG1 |
| Immunogen: | Synthetic peptide corresponding to 30 aa (GPPAAAPGHPLAPGPHPAAPSSWGPRPRRY) from the first N-terminal D1 domain of human LAG-3 (lymphocyte activation gene-3). |
| UniProt ID: | P18627 |
| Source: | Purified from hybridoma tissue culture supernatant. |
| Species reactivity: | Human |
| Specificity: | Recognizes the 30 aa extra-loop of the first N-terminal D1 domain of LAG-3. |
| Applications: | Flow Cytometry, ICC |
| Recommended Dilutions/Conditions: | Flow Cytometry (1:100) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
| Purity Detail: | Purified from concentrated hybridoma tissue culture supernatant by Protein A Sepharose™ CL-4B Flow Fast Column. |
| Formulation: | Liquid. In PBS containing 0.1M sodium bicarbonate, 50mM Tris, 1% BSA and 0.02% sodium azide. |
| Use/Stability: | Stable for at least 6 months after receipt when stored as recommended. |
| Handling: | Avoid vigorous centrifugation and vortexing. Do not freeze. Protect from light. |
| Shipping: | Blue Ice Not Frozen |
| Short Term Storage: | +4°C |
| Long Term Storage: | +4°C |
| Scientific Background: | The lymphocyte activation gene-3 (LAG-3, CD223), a member of the immunoglobulin superfamily (IgSF) related to CD4, binds to the major histocompatibility complex (MHC) class II molecules but with higher affinity than CD4. Several alternative mRNA splice-variants of human LAG-3 have been described, two of them encoding potential secreted forms: LAG-3V1 (i.e. the D1-D2 domains of the protein, 36 kDa) and LAG-3V3 (D1-D3, 52 kDa). The longer form was detected by ELISA in the serum of healthy individuals as well as of tuberculosis patients with a favorable outcome. LAG-3 expression by T cell clones correlated with IFN-γ production, and hence soluble LAG-3 has been suggested as a serological marker of Th1 responses. |
| Technical Info/Product Notes: | 1 test means: 1µl of MAb is used to stain 500'000 cells in a sample volume of 50µl. ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. ATTO 488 shows bright green fluorescence (λabs (max): 501nm, λem (max): 523nm, εmax: 90'000). SEPHAROSE is a trademark of GE Healthcare companies. |
| Regulatory Status: | RUO - Research Use Only |
LAG-3 (human) monoclonal antibody (17B4) (ATTO 647N conjugate) |
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| Purified from hybridoma tissue culture supernatant., Flow Cytometry, ICC | Print as PDF | ||||||
| ALX-804-806TS-T100 | 100 tests | ATTO 647N | Inquire for pricing | |||
| Do you need bulk/larger quantities? | ||||||
LAG-3 (human) monoclonal antibody (17B4) (FITC conjugate) |
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| Purified from hybridoma tissue culture supernatant., Flow Cytometry | Print as PDF | ||||||
| ALX-804-806F-C100 | 100 µg | FITC | Inquire for pricing | |||
| Do you need bulk/larger quantities? | ||||||
LAG-3 (human) monoclonal antibody (17B4) (biotin conjugate) |
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| Purified from hybridoma tissue culture supernatant., ELISA, WB | Print as PDF | ||||||
| ALX-804-806B-C100 | 100 µg | Biotin | Inquire for pricing | |||
| Do you need bulk/larger quantities? | ||||||
LAG-3 (human) monoclonal antibody (17B4) |
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| Purified from hybridoma tissue culture supernatant., ICC, IHC, IP, WB | Print as PDF | ||||||
| ALX-804-806-C100 | 100 µg | Inquire for pricing | ||||
| Do you need bulk/larger quantities? | ||||||
