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MEKK1 polyclonal antibody

ENZ-ABS293-0100 100 µg 471.00 USD
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Product Details

Alternative Name:MAP3K1, MEK kinase 1, Mitogen-activated protein kinase kinase kinase 1, MAPK/ERK kinase kinase 1, MAPKKK1
Immunogen:Recombinant MEKK1 (aa 1307-1509).
UniProt ID:Q13233
Species reactivity:Human
Applications:ELISA, ICC, IHC (FS), IHC (PS), WB
Recommended Dilutions/Conditions:ELISA (1:100-200)
Immunocytochemistry (formalin fixed cells) (1:100-500)
Immunohistochemistry (frozen sections) (1:100-500)
Immunohistochemistry (paraffin sections) (1:50-200)
Western blot (1:100-400)

Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~26KDa by Western blot.
Positive Control:Included.
Purity Detail:Affinity purified.
Formulation:Liquid. In PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:MEKKs (Mitogen activated protein kinase kinase kinases) are serine-threonine kinases that act as the first tier of cellular MAP kinase pathways by activation of MAP/ERK kinases, or MEKs. Many enzymes with MEKK activity have been identified, including MEKK1-4, Raf, MLK3, TAK, and DLK. MEKKs generally display little similarity outside of their catalytic kinase domains. MEKK1-4 are nearly 50% identical within their catalytic domains, and are known to regulate Erk, Jnk, and p38 MAP kinase pathways. MEKK2 and MEKK3 bind MEK5 via conserved PB1 domains, leading to downstream activation of Erk5.
Regulatory Status:RUO - Research Use Only
MEKK1 polyclonal antibody Western blot
Figure 1. Western Blot analysis using MEKK1, pAb (Prod. No. ENZ-ABS293).
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MEKK1 polyclonal antibody Western blot

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