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FLUOR DE LYS®-Succinyl, Desuccinylase Substrate

 
BML-KI590-0050 50 µl 206.00 USD
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The FLUOR DE LYS®-Succinyl, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5.  The assay procedure has two steps.  First, the FLUOR DE LYS®- Succinyl, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.).  Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® Developer (Prod. No. BML-KI105) produces a free AMC fluorophore which can be monitored with standard fluorometers (Ex. 350-380nm / Em. 450-480nm).

Product Details

Source:Synthetic.
 
Concentration:5 mM
 
Formulation:Solution in DMSO.
 
Excitation maximum:350-380nm
 
Emission maximum:450-480nm
 
Purity:≥95% (HPLC)
 
Appearance:Liquid.
 
Application Notes:Can be used for the study of desuccinylase kinetics, regulation, and inhibitor sensitivity. Ideal for drug discovery and HTS applications.
 
Shipping:Shipped on Dry Ice
 
Short Term Storage:-20°C
 
Long Term Storage:-80°C
 
Use/Stability:Stable for 1 year when stored at -80°C.
 
Technical Info/Product Notes:NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105).  For assay conditions, see the product manual for BML-AK513 SIRT5 Fluorimetric Drug Discovery Kit.
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Identification of Diketopiperazine-Containing 2-Anilinobenzamides as Potent Sirtuin 2 (SIRT2)-Selective Inhibitors Targeting the "Selectivity Pocket", Substrate-Binding Site, and NAD+-Binding Site: P. Mellini, et al.; J. Med. Chem. 62, 5844 (2019), Abstract;

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