Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

FLUOR DE LYS®-Succinyl, Desuccinylase Substrate

BML-KI590-0050 50 µl 231.00 USD
Do you need bulk/larger quantities?
The FLUOR DE LYS®-Succinyl, Desuccinylase Substrate offers a highly sensitive and convenient alternative to radiolabeled, succinylated histones or peptide/HPLC methods for the assay of desuccinylases such as SIRT5.  The assay procedure has two steps.  First, the FLUOR DE LYS®- Succinyl, Desuccinylase Substrate, which comprises a succinylated lysine side chain, is incubated with a sample containing desuccinylase activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.).  Desuccinylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® Developer (Prod. No. BML-KI105) produces a free AMC fluorophore which can be monitored with standard fluorometers (Ex. 350-380nm / Em. 450-480nm).

Product Details

Concentration:5 mM
Formulation:Solution in DMSO.
Excitation maximum:350-380nm
Emission maximum:450-480nm
Purity:≥95% (HPLC)
Application Notes:Can be used for the study of desuccinylase kinetics, regulation, and inhibitor sensitivity. Ideal for drug discovery and HTS applications.
Shipping:Dry Ice
Short Term Storage:-20°C
Long Term Storage:-80°C
Use/Stability:Stable for 1 year when stored at -80°C.
Technical Info/Product Notes:NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105).  For assay conditions, see the product manual for BML-AK513 SIRT5 Fluorimetric Drug Discovery Kit.
Regulatory Status:RUO - Research Use Only

Product Literature References

Identification of Diketopiperazine-Containing 2-Anilinobenzamides as Potent Sirtuin 2 (SIRT2)-Selective Inhibitors Targeting the "Selectivity Pocket", Substrate-Binding Site, and NAD+-Binding Site: P. Mellini, et al.; J. Med. Chem. 62, 5844 (2019), Abstract;

Related Products

FLUOR DE LYS® developer I concentrate 

An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate.
Print as PDF
BML-KI105-0300 300 µl 196.00 USD
Do you need bulk/larger quantities?

FLUOR DE LYS®-Green substrate 

≥95% (HPLC) | Print as PDF
BML-KI572-0050 50 µl 245.00 USD
Do you need bulk/larger quantities?

FLUOR DE LYS® SIRT5 fluorometric drug discovery assay kit 

A Sensitive Fluorometric Assay for Screening SIRT5 Inhibitors
Fluorescent detection, HTS, Activity assay, Cell-based assays | Print as PDF
BML-AK513-0001 100 tests 791.00 USD
Do you need bulk/larger quantities?

Product Toolbox


Certificate of Analysis


By target:
By biological activity:
HDAC Substrate
By catalog section:


Technical Service
Customer Service

Related Literature

Stem Cells
Stem Cells
Download as PDF

Download as PDF

All new literature pieces