Online Purchasing Account You are logged on as Guest. LoginRegister a New AccountShopping cart (Empty)
United States 

FLUOR DE LYS®-Green substrate

 
BML-KI572-0050 50 µl 229.00 USD
Do you need bulk/larger quantities?
 
The FLUOR DE LYS®-Green substrate offers a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The assay procedure has two steps. First, the FLUOR DE LYS®-Green Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (HeLa nuclear or other extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the FLUOR DE LYS® Developer (Prod. No. BML-KI105) produces a fluorophore. The FLUOR DE LYS®-Green substrate has a higher wavelength excitation and emission than the original FLUOR DE LYS® substrate (Prod. No. BML-KI104). This avoids interference by quenching or fluorescence from compounds absorbing and/or emitting in the near UV and blue range.

Product Details

Quantity:2.5 µmol
 
Formulation:50mM solution in DMSO.
 
Purity:≥95% (HPLC)
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Technical Info/Product Notes: NOTE: Must be used in conjunction with FLUOR DE LYS® Developer (Prod. No. BML-KI105; see attached assay conditions).
 
Regulatory Status:RUO - Research Use Only
 

Product Literature References

Biphenyl-4-yl-acrylohydroxamic acids: Identification of a novel indolyl-substituted HDAC inhibitor with antitumor activity: R. Cincinelli, et al.; Eur. J. Med. Chem. 112, 99 (2016), Abstract;
4-(1-Ethyl-4-anisyl-imidazol-5-yl)-N-hydroxycinnamide–A new pleiotropic HDAC inhibitor targeting Cancer cell signalling and cytoskeletal organisation: K. Mahal, et al.; Exp. Cell Res. 336, 263 (2015), Application(s): HDAC Assay, Abstract;

Related Products

FLUOR DE LYS® developer I concentrate 

An essential reagent for signal generation in HDAC or sirtuin assays done with the FLUOR DE LYS® substrate.
Print as PDF
 
BML-KI105-0300 300 µl 183.00 USD
Do you need bulk/larger quantities?
 

Product Toolbox

PRODUCT RESOURCES

Datasheet
SDS
Certificate of Analysis
Manual

RELATED PRODUCTS

By target:
HDAC
By biological activity:
HDAC Substrate
By catalog section:

PRODUCT SUPPORT

FAQs
Technical Service
Customer Service

Related Literature

Brochures
Stem Cells
Stem Cells
Download as PDF

Brochures
Epigenetics
Epigenetics
Download as PDF

All new literature pieces

Keep in touch

©2022 Enzo Life Sciences, Inc.,