Replaces Prod. #: ALX-350-014
Potent cell permeable inhibitor with high specificity for protein phosphatase 1 (PP1) and 2A (PP2A). Inhibits p130cas tyrosine phosphorylation. Enhances the phosphorylation level of NF-κB. Affects intracellular signalling processes that require 14-3-3. Potent non-phorbol type tumor promoter. Prevents γ-radiation induced apoptosis. Stimulates contraction of smooth muscle, induces intracellular protein phosphorylation in cultured human keratinocytes. Inhibits apoptosis. No effect on acid or alkaline phosphatases at 1µM. Treatment of MCF7 cells (breast cancer line), with 100nM, produced rapid (<20 min.) but incomplete inhibition of both PP1 (~60%) and PP2A (~70%).
Product Details
| Formula: | C50H81N4O15P |
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| MW: | 1009.2 |
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| CAS: | 101932-71-2 |
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| Purity: | ≥98% (HPLC) |
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| Appearance: | Clear film or white crystalline powder. |
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| Solubility: | Soluble in DMSO, 100% ethanol and dimethyl formamide. |
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| Shipping: | Ambient |
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| Long Term Storage: | -20°C |
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| Use/Stability: | Stable for at least 1 year after receipt when stored, as supplied, at -20°C. Stock solutions are stable for up to 3 months at -20°C. |
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| Regulatory Status: | RUO - Research Use Only |
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Product Literature References
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MAP4K4 expression in cardiomyocytes: multiple isoforms, multiple phosphorylations and interactions with striatins: S.J. Fuller, et al.; Biochem. J.
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Full Text
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Inhibition of p130cas tyrosine phosphorylation by calyculin A: W. Qiu, et al.; J. Leukoc. Biol.
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Abstract;
Differential inhibition and posttranslational modification of protein phosphatase 1 and 2A in MCF7 cells treated with calyculin-A, okadaic acid, and tautomycin: B. Favre, et al.; J. Biol. Chem.
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Abstract;
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Calyculin A, a non-phorbol ester type tumor promotor, induced oxidative DNA damage in stimulated human neutrophil-like cells: T. Takeuchi, et al.; BBRC
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Potassium and calcium channel involvement in induction of long-lasting synaptic enhancement by calyculin A, a protein phosphatase inhibitor, in rat hippocampal CA1 region: N. Murakami, et al.; Neurosci. Lett.
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Calyculin A, a potent inhibitor of phosphatases-1 and -2A, prevents apoptosis: Q. Song & M.F. Lavin; BBRC
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Nonphorbol tumor promoters okadaic acid and calyculin-A induce membrane translocation of protein kinase C: R. Gopalakrishna; BBRC
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Codon 61 mutations in the c-Harvey-ras gene in mouse skin tumors induced by 7,12-dimethylbenz[a]anthracene plus okadaic acid class tumor promoters: H. Fujiki, et al.; Mol. Carcinog.
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Full Text
The structure and regulation of protein phosphatases: P. Cohen; Ann. Rev. Biochem.
58, 453 (1989),
Abstract;
Okadaic acid: an additional non-phorbol-12-tetradecanoate-13-acetate- type tumor promoter: M. Suganuma, et al.; PNAS
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