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[pSer239]VASP monoclonal antibody (16C2) (biotin conjugate)

ALX-804-240B-C100 100 µg Biotin 929.00 USD
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Product Details

Alternative Name:Vasodilator stimulated phosphoprotein
Immunogen:Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH.
UniProt ID:P50552
Species reactivity:Human, Mouse, Rat
Specificity:Recognizes Ser239-phosphorylated VASP.
Crossreactivity:Does not cross-react with the non-phosphorylated form of VASP.
Applications:ELISA, Flow Cytometry, ICC, IP, WB
Recommended Dilutions/Conditions:ELISA (use at 0.05µg/ml)
Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation)
Immunoprecipitation (use 1-10 µg per 106 pervanadate-treated A431 cells)
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Thiophilic adsorption and size exclusion chromatography purified.
Formulation:Liquid. 0.5mg/ml in 2x PBS containing 0.09% sodium azide, PEG and sucrose.
Use/Stability:Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months.
Handling:Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen.
Shipping:Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:Important! The use of MAb to VASP (activated) (Ser239) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only!
Regulatory Status:RUO - Research Use Only

Product Literature References

Cyclic nucleotides modulate store-mediated calcium entry through the activation of protein-tyrosine phosphatases and altered actin polymerization in human platelets: J.A. Rosado, et al.; J. Biol. Chem. 276, 15666 (2001), Abstract; Full Text
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Simulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation 103, 2188 (2001), Abstract; Full Text
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem. 275, 33536 (2000), Abstract; Full Text
Flow cytometry analysis of intracell. VASP phosphorylation for the assessment of activating and inhibitory signal transduct. pathways in human platelets-definition & detect. of ticlopidine/clopidogrel: U.R. Schwarz, et al.; Thromb. Haemost. 82, 1145 (1999), Abstract;
Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody: A. Smolenski, et al.; J. Biol. Chem. 273, 20029 (1998), Abstract; Full Text

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