Product Details
Alternative Name: | Vasodilator stimulated phosphoprotein |
|
Clone: | 16C2 |
|
Host: | Mouse |
|
Isotype: | IgG1 |
|
Immunogen: | Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH. |
|
UniProt ID: | P50552 |
|
Species reactivity: | Human, Mouse, Rat Rabbit
|
|
Specificity: | Recognizes Ser239-phosphorylated VASP. |
|
Crossreactivity: | Does not cross-react with the non-phosphorylated form of VASP. |
|
Applications: | ELISA, Flow Cytometry, ICC, IP, WB
|
|
Recommended Dilutions/Conditions: | ELISA (use at 0.05µg/ml) Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation) Immunoprecipitation (use 1-10 µg per 106 pervanadate-treated A431 cells) Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
|
Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
|
Formulation: | Liquid. 0.5mg/ml in 2x PBS containing 0.09% sodium azide, PEG and sucrose. |
|
Use/Stability: | Reconstituted antibody is stable for 1 year when stored at -80°C. Thaw aliquots at +37°C. Thawed aliquots may be stored at +4°C for up to 3 months. |
|
Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
|
Shipping: | Shipped on Blue Ice |
|
Long Term Storage: | -20°C |
|
Technical Info/Product Notes: | Important! The use of MAb to VASP (activated) (Ser239) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only! |
|
Regulatory Status: | RUO - Research Use Only |
|
Product Literature References
Cyclic nucleotides modulate store-mediated calcium entry through the activation of protein-tyrosine phosphatases and altered actin polymerization in human platelets: J.A. Rosado, et al.; J. Biol. Chem.
276, 15666 (2001),
Abstract;
Full Text
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Simulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation
103, 2188 (2001),
Abstract;
Full Text
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem.
275, 33536 (2000),
Abstract;
Full Text
Flow cytometry analysis of intracell. VASP phosphorylation for the assessment of activating and inhibitory signal transduct. pathways in human platelets-definition & detect. of ticlopidine/clopidogrel: U.R. Schwarz, et al.; Thromb. Haemost.
82, 1145 (1999),
Abstract;
Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody: A. Smolenski, et al.; J. Biol. Chem.
273, 20029 (1998),
Abstract;
Full Text
Related Products