Product Details
| Alternative Name: | Vasodilator stimulated phosphoprotein |
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| Clone: | 16C2 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to a portion of VASP (vasodilator stimulated phosphoprotein) phosphorylated at Ser239 (RKVpSKQE) conjugated to KLH. |
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| UniProt ID: | P50552 |
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| Species reactivity: | Human, Mouse, Rat
Rabbit
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| Specificity: | Recognizes Ser239-phosphorylated VASP. |
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| Crossreactivity: | Does not cross-react with the non-phosphorylated form of VASP. |
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| Applications: | ELISA, Flow Cytometry, ICC, IP, WB
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| Recommended Dilutions/Conditions: | ELISA (use at 0.05µg/ml)
Immunocytochemistry (use at 1-10µg/ml; may tolerate 0.5% formaldehyde fixation) , Immunoprecipitation (use 1-10 µg per 106 pervanadate-treated A431 cells)
Western Blot (1µg/ml for AP/BCIP/NBT (MTT) detection; 0.5µg/ml for HRPO/ECL detection; recommended blocking buffer BPPT: 2x PBS 3% (w/v) BSA, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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| Formulation: | Lyophilized from 1ml of 2x PBS containing 0.09% sodium azide, PEG, and sucrose. |
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| Reconstitution: | Reconstitute with 1ml water (15 minutes at room temperature). |
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| Use/Stability: | Stable at -80°C up to 1 year, at 4°C up to 3 months. |
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| Handling: | Avoid freeze/thaw cycles. After reconstitution, prepare aliquots and freeze in liquid nitrogen. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Technical Info/Product Notes: | Important! The use of MAb to VASP (activated) (Ser239) (16C2) for diagnostic or therapeutic purposes is patented! The antibody is supplied for research use only! |
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| Regulatory Status: | RUO - Research Use Only |
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![[pSer239]VASP monoclonal antibody (16C2) Western blot](https://www.enzolifesciences.com/fileadmin/files/image/804-240.gif "[pSer239]VASP monoclonal antibody (16C2) Western blot")
Figure: Western blot analysis to illustrate phosphospecificity.Whole cell extracts of control (co) or Forskolin (F) treated MDA-MB-231 tumor cells were applied to SDS-PAGE (ca. 20’000 cells per lane) and transfereed to PVDF membranes. Immunoblots were probed with MAb to VASP (phosphorylated) (pSer239) (16C2) (Prod. No.
ALX-804-240) for 1h at RT and developed by ECL (exp. time: 30 s)."
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Product Literature References
A conjugate of decyltriphenylphosphonium with plastoquinone can carry cyclic adenosine monophosphate, but not cyclic guanosine monophosphate, across artificial and natural membranes: A.M. Firsov, et al.; Biochim. Biophys. Acta
1860, 329 (2018),
Abstract;
Dual roles of quercetin in platelets: phosphoinositide-3-kinase and MAP kinases inhibition, and cAMP-dependent vasodilator-stimulated phosphoprotein stimulation: W.J. Oh, et al.; Evid. Based Complement Alternat. Med.
2012, 485262 (2012),
Application(s): Western blot,
Abstract;
Full Text
Acyl derivatives of coenzyme A inhibit platelet function via antagonism at P2Y1 and P2Y12 receptors: a new finding that may influence the design of anti-thrombotic agents: P. Manolopoulos, et al.; Platelets
19, 134 (2008),
Abstract;
Full Text
The active metabolite of prasugrel effectively blocks the platelet P2Y12 receptor and inhibits procoagulant and pro-inflammatory platelet responses: H.M. Judge, et al.; Platelets
19, 125 (2008),
Abstract;
Dual actions of dephostatin on the nitric oxide/cGMP-signalling pathway in porcine iliac arteries: A.A. Persson, et al.; Eur. J. Pharmacol.
521, 124 (2005),
Application(s): Western blot,
Abstract;
Characterisation of GEA 3175 on human platelets;: comparison with S-nitroso-N-acetyl-D, L-penicillamine: A.K. Asplund Persson, et al.; Eur. J. Pharmacol.
496, 1 (2004),
Application(s): Western blot,
Abstract;
The activity of constitutive nitric oxide synthase is increased by the pathway cAMP/cAMP-activated protein kinase in human platelets. New insights into the antiaggregating effects of cAMP-elevating agents.: I. Russo, et al.; Thromb. Res.
114, 265 (2004),
Application(s): Western blot,
Abstract;
Cyclic nucleotides modulate store-mediated calcium entry through the activation of protein-tyrosine phosphatases and altered actin polymerization in human platelets: J.A. Rosado, et al.; J. Biol. Chem.
276, 15666 (2001),
Abstract;
Full Text
Effects of In Vivo Nitroglycerin Treatment on Activity and Expression of the Guanylyl Cyclase and cGMP-Dependent Protein Kinase and Their Downstream Target Vasodilator-Simulated Phosphoprotein in Aorta: A. Mülsch, et al.; Circulation
103, 2188 (2001),
Abstract;
Full Text
KT5823 inhibits cGMP-dependent protein kinase activity in vitro but not in intact human platelets and rat mesangial cells: M. Burkhardt, et al.; J. Biol. Chem.
275, 33536 (2000),
Abstract;
Full Text
Flow cytometry analysis of intracell. VASP phosphorylation for the assessment of activating and inhibitory signal transduct. pathways in human platelets-definition & detect. of ticlopidine/clopidogrel: U.R. Schwarz, et al.; Thromb. Haemost.
82, 1145 (1999),
Abstract;
Analysis and Regulation of Vasodilator-stimulated Phosphoprotein Serine 239 Phosphorylation in Vitro and in Intact Cells Using a Phosphospecific Monoclonal Antibody: A. Smolenski, et al.; J. Biol. Chem.
273, 20029 (1998),
Abstract;
Full Text
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![[pSer239]VASP monoclonal antibody (16C2) Western blot](https://www.enzolifesciences.com/fileadmin/thumbs/5d495d2fd07046060b614529918c1ad219b5f46b-804-240.gif.jpg "[pSer239]VASP monoclonal antibody (16C2) Western blot")
