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PPH . hydrochloride

Spin trapping reagent
ALX-430-080-M010 10 mg 130.00 USD
ALX-430-080-M050 50 mg 501.00 USD
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Effective, non-cell permeable and non-toxic analog of CPH for the detection of superoxide radicals. Resistant against reduction of vitamin C. For cell permeable analog see CPH (Prod. No. ALX-430-078).

Product Details

Alternative Name:1-Hydroxy-4-phosphono-oxy-2,2,6,6-tetramethylpiperidine . HCl
Formula:C9H20NO5P . HCl
MW:253.2 . 36.5
Purity:≥99% (EPR)
Purity Detail:≤0.1% paramagnetic impurities (EPR)
Appearance:White to off-white solid.
Solubility:Soluble in DMSO, 100% ethanol, methanol or water.
Shipping:Blue Ice
Long Term Storage:-20°C
Technical Info/Product Notes:The stabilized acid form of PPH is not well soluble at neutral pH (~0.1-0.01%). It is recommended to adjust pH to 7.4 for higher solubility. Dissolve PPH in oxygen-free buffers at neutral pH and use 1M NaOH to adjust pH to 7.4. Solubility is 1% in water, 0.8% in methanol or 0.5% in ethanol at pH 7.4. Solubility is 3% in DMSO at pH 7.4 (first add NaOH to PPH crystals then DMSO). Higher concentrations require further pH adjustment using NaOH.
Regulatory Status:RUO - Research Use Only
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Product Literature References

EPR detection of cellular and mitochondrial superoxide using cyclic hydroxylamines: S.I. Dikalov, et al.; Free Rad. Res. 45, 417 (2011), Abstract;
Molecular Mechanisms of Angiotensin II Mediated Mitochondrial Dysfunction. Linking Mitochondrial Oxidative Damage and Vascular Endothelial Dysfunction: A.K. Doughan, et al.; Circ. Res. 102, 488 (2008), Abstract;
Rotenone model of Parkinson disease: multiple brain mitochondria dysfunctions after short term systemic rotenone intoxication: A. Panov, et al.; J. Biol. Chem. 280, 42026 (2005), Abstract;
Noninvasive diagnostic tool for inflammation-induced oxidative stress using electron spin resonance spectroscopy and an extracellular cyclic hydroxylamine: S.I. Dikalov, et al.; Arch. Biochem. Biophys. 402, 218 (2002), Abstract;
A new approach for extracellular spin trapping of nitroglycerin-induced superoxide radicals both in vitro and in vivo: B. Fink, et al.; Free Radic. Biol. Med. 28, 121 (2000), Abstract;
Detection of superoxide radicals and peroxynitrite by 1-hydroxy-4- phosphonooxy-2,2,6,6-tetramethylpiperidine: quantification of extracellular superoxide radicals formation: S. Dikalov, et al.; BBRC 248, 211 (1998), Abstract;

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