Product Details
Alternative Name: | RIG-1, Retinoic acid-inducible gene 1 protein, DEAD-box protein 58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 M, Probable ATP-dependent RNA helicase DDX58 |
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Host: | Rabbit |
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Immunogen: | Recombinant human RIG-I (aa 201-713). |
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UniProt ID: | O95786 |
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Source: | Purified from rabbit serum. |
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Species reactivity: | Human
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Applications: | IP, WB
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Recommended Dilutions/Conditions: | Immunoprecipitation (1:100) Western blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A-affinity purified. |
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Formulation: | Liquid. In PBS containing 0.02% sodium azide. |
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Use/Stability: | Stable for at least 1 year after receipt when stored at -20°C. |
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Handling: | Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C. |
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Shipping: | Shipped on Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Scientific Background: | RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells: X. Yin, et al.; Cell Rep.
32, 108628 (2021),
Application(s): Western Blot,
Abstract;
Full Text
Association of a Network of Interferon-Stimulated Genes with a Locus Encoding a Negative Regulator of Non-conventional IKK Kinases and IFNB1: S. Jeidane, et al.; Cell Rep.
17, 425 (2016),
Application(s): Western Immunoblot Analyses,
Abstract;
Full Text
ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA: C. Lässig, et al.; eLife
4, e10859 (2015),
Application(s): Dependent signaling,
Abstract;
Full Text
Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: roles of TRAF3 and TRAF5: Y. Chiba, et al.; Biochem. Biophys. Res. Commun.
467, 191 (2015),
Application(s): Immunoblot analyses of human tissue,
Abstract;
Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils: N. Tamassia, et al.; J. Immunol.
181, 6563 (2008),
Abstract;
General Literature References
5’-Triphosphate RNA is the ligand for RIG-I: V. Hornung, et al.; Science
314, 994 (2006),
Abstract;
RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates: A. Pichlmair, et al.; Science
314, 997 (2006),
Abstract;
Virology. Sensing viral RNA amid your own: T. Fujita; Science
314, 935 (2006),
Abstract;
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