RIG-I (human) polyclonal antibody (AT111)



ALX-210-932-C100	100 µg	386.00 USD
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Product Details

Alternative Name:	RIG-1, Retinoic acid-inducible gene 1 protein, DEAD-box protein 58, DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 M, Probable ATP-dependent RNA helicase DDX58

Host:	Rabbit

Immunogen:	Recombinant human RIG-I (aa 201-713).

UniProt ID:	O95786

Source:	Purified from rabbit serum.

Species reactivity:	Human

Applications:	IP, WB

Recommended Dilutions/Conditions:	Immunoprecipitation (1:100) Western blot (1:1,000) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application.

Purity Detail:	Protein A-affinity purified.

Formulation:	Liquid. In PBS containing 0.02% sodium azide.

Use/Stability:	Stable for at least 1 year after receipt when stored at -20°C.

Handling:	Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20°C.

Shipping:	Blue Ice

Short Term Storage:	+4°C

Long Term Storage:	-20°C

Scientific Background:	RIG-I (retinoic acid-inducible gene I; Ddx58), a member of the DEAD-box RNA helicases, is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.

Regulatory Status:	RUO - Research Use Only

Product Literature References

MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells: X. Yin, et al.; Cell Rep. 32, 108628 (2021), Application(s): Western Blot, Abstract; Full Text

Association of a Network of Interferon-Stimulated Genes with a Locus Encoding a Negative Regulator of Non-conventional IKK Kinases and IFNB1: S. Jeidane, et al.; Cell Rep. 17, 425 (2016), Application(s): Western Immunoblot Analyses, Abstract; Full Text

ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA: C. Lässig, et al.; eLife 4, e10859 (2015), Application(s): Dependent signaling, Abstract; Full Text

Retinoic acid-inducible gene-I-like receptor (RLR)-mediated antiviral innate immune responses in the lower respiratory tract: roles of TRAF3 and TRAF5: Y. Chiba, et al.; Biochem. Biophys. Res. Commun. 467, 191 (2015), Application(s): Immunoblot analyses of human tissue, Abstract;

Activation of an immunoregulatory and antiviral gene expression program in poly(I:C)-transfected human neutrophils: N. Tamassia, et al.; J. Immunol. 181, 6563 (2008), Abstract;