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Cardif (human) polyclonal antibody (AT107)

 
ALX-210-929-C100 100 µg 421.00 USD
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Product Details

Alternative Name:CARD adapter inducing interferon-β, IPS-1, Interferon-β promoter stimulator protein 1, MAVS, Mitochondrial antiviral signalling protein, VISA, Virus-induced signalling adapter
 
Host:Rabbit
 
Immunogen:Recombinant human Cardif (CARD adapter inducing interferon-β) (aa 160-450).
 
UniProt ID:Q7Z434
 
Source:Purified from rabbit serum.
 
Species reactivity:Human
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (1:500)
Immunoprecipitation (1:100)
Western Blot (1:2,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~65kDa by Western blot.
 
Purity Detail:Protein A-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Use/Stability:Stable for at least 1 year after receipt when stored at -20°C.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
Scientific Background:RIG-I (retinoic acid-inducible gene I; Ddx58) and Mda5 (melanoma differentiation-associated gene 5, also known as Ifih1 or Helicard) are proteins that sense viral replication intermediates, such as double-stranded RNA and triggers the host antiviral programs. These molecules signal the downstream activation of NF-κB and IFN regulatory factor (IRF) -3, which coordinately regulate the expression of type-I interferons. Cardif (also called VISA/IPS-1/MAVS) is a CARD (caspase activation and recruitment domain)-containing adaptor protein that interacts with the CARD domain of RIG-I and Mda5, leading to the activation of NF-κB and IRF3. Cardif is located to the mitochondrial outer membrane. Removal of the mitochondrial-targeting domain of cardif abolishes its ability to induce IFNs. Cardif is cleaved and inactivated by NS3-4A, a serine protease from hepatitis C virus known to block interferon-β production.
 
Regulatory Status:RUO - Research Use Only
 
Cardif (human) polyclonal antibody (AT107) Western blot
Western blot analysis of Cardif (human), pAb (AT107) (Prod. No. ALX-210-929): Lane 1: MW marker; Lane 2: HepG2; Lane 3: PALA; and Lane 4: HeLa. Additional bands observed probably represent isoforms or cleaved products of Cardif.
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Cardif (human) polyclonal antibody (AT107) Western blot

Product Literature References

MDA5 Governs the Innate Immune Response to SARS-CoV-2 in Lung Epithelial Cells: X. Yin, et al.; Cell Rep. 32, 108628 (2021), Application(s): Western Blot, Abstract; Full Text
DHX15 Is a Coreceptor for RLR Signaling That Promotes Antiviral Defense Against RNA Virus Infection: S. Pattabhi, et al.; J. Interferon Cytokine Res. 39, 331 (2019), Abstract;
The 14-3-3η chaperone protein promotes antiviral innate immunity via facilitating MDA5 oligomerization and intracellular redistribution: J.P. Lin, et al.; PLoS Pathog. 15, e1007582 (2019), Abstract; Full Text
Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling: J. Hertzog, et al.; Eur. J. Immunol. 48, 1120 (2018), Abstract; Full Text
Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy: G.L. McLelland, et al.; Elife 7, e32866 (2018), Abstract; Full Text
NSs Protein of Sandfly Fever Sicilian Phlebovirus Counteracts Interferon (IFN) Induction by Masking the DNA-Binding Domain of IFN Regulatory Factor 3: J.D. Wuerth, et al.; J. Virol. 92, e01202-18 (2018), Abstract; Full Text
Palmitoylation mediates membrane association of hepatitis E virus ORF3 protein and is required for infectious particle secretion: J. Gouttenoire, et al.; PLoS Pathog. 14, e1007471 (2018), Abstract;
DDX3 directly regulates TRAF3 ubiquitination and acts as a scaffold to coordinate assembly of signalling complexes downstream of MAVS: L. Gu, et al.; Biochem. J. 474, 571 (2017), Abstract;
Disruption of MDA5-Mediated Innate Immune Responses by the 3C Proteins of Coxsackievirus A16, Coxsackievirus A6, and Enterovirus D68: Y. Rui, et al.; J. Virol. 91, e00546-17 (2017), Abstract; Full Text
NLRX1 promotes immediate IRF1-directed antiviral responses by limiting dsRNA-activated translational inhibition mediated by PKR: H. Feng, et al.; Nat. Immunol. 18, 1299 (2017), Abstract; Full Text
Cleavage of mitochondrial antiviral signaling protein in the liver of patients with chronic hepatitis C correlates with a reduced activation of the endogenous interferon system: P. Bellecave, et al.; Hepatology 51, 1127 (2010), Abstract;
Cleavage of the IPS-1/Cardif/MAVS/VISA does not inhibit T cell-mediated elimination of hepatitis C virus non-structural 3/4A-expressing hepatocytes: G. Ahlen, et al.; Gut 58, 560 (2009), Abstract;
Antiviral suppression vs restoration of RIG-I signaling by hepatitis C protease and polymerase inhibitors: Y. Liang, et al.; Gastroenterology 135, 1710 (2008), Abstract;
Distinct RIG-I and MDA5 signaling by RNA viruses in innate immunity: Y.M. Loo, et al.; J. Virol. 82, 335 (2008), Abstract;
The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008), Abstract;
Regulation of antiviral responses by a direct and specific interaction between TRAF3 and Cardif : S. K. Saha; EMBO J. 25, 3257 (2006), Abstract;

General Literature References

CARD games between virus and host get a new player: C.L. Johnson and M. Gale, Jr.; Trends Immunol. 27, 1 (2006), Abstract;
Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005), Abstract;
Identification and characterization of MAVS, a mitochondrial antiviral signaling protein that activates NF-kappaB and IRF 3: R.B. Seth, et al.; Cell 122, 669 (2005), Abstract;
IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction: T. Kawai, et al.; Nat. Immunol. 6, 981 (2005), Abstract;
VISA is an adapter protein required for virus-triggered IFN-beta signaling: L.G. Xu, et al.; Mol. Cell 19, 727 (2005), Abstract;

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