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NFκB p65 polyclonal antibody

ADI-KAS-TF110-D 50 µg 213.00 USD
ADI-KAS-TF110-F 200 µg 470.00 USD
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Product Details

Alternative Name:RELA
Immunogen:Synthetic peptide corresponding to a portion of human p65 NF-κB. The sequence is completely conserved in mouse NF-κB p65.
UniProt ID:Q04206
GenBank ID:M62399
Source:Purified from rabbit serum.
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Guinea pig, Monkey, Porcine, Rabbit
Recommended Dilutions/Conditions:Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~65kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Liquid. In PBS, pH 7.2, containing 0.09% sodium azide.
Handling:Avoid freeze/thaw cycles.
Shipping:Blue Ice
Long Term Storage:-20°C
Scientific Background:Nuclear factor kappa-B (NFk) B) functions as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NF-kB is a heterodimer consisting of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA) (ref. 3), both of which exhibit sequence homology to the protooncogene c-Rel. p50 has an isoform called p49/p52, and both proteins derive from the amino-terminal of precursor protein p105 and p100, respectively. The p50/p65 heterodimer remains in inactive form in the cytosol as a complex with its inhibitor, IkB. Upon stimulation of cells by a variety of stimuli, including lipopolysaccharide (LPS), pro-inflammatory cytokines like interleukin-1 and tumor necrosis factor, and viral infection, IkB becomes phosphorylated and degraded by the proteosome. The active NF-kB heterodimer translocates into the nucleus and induces gene expression.
Regulatory Status:RUO - Research Use Only

Product Literature References

Effect of EGCG on bronchial epithelial cell premalignant lesions induced by cigarette smoke and on its CYP1A1 expression: Q. Gu, et al.; Int. J. Mol. Med. 48, 220 (2021), Abstract;
TNF Apoptosis Protection Fraction (TAPF) prevents apoptosis induced by TNF, but not by Fas or TRAIL, via NF-κB-induced increase in cFLIP: J.Y. Seol, et al.; Cytokine 75, 321 (2015), Application(s): Western Blot, Abstract;

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