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PI3 kinase monoclonal antibody (AB6)

 
ADI-KAM-PI200-E 100 µg 315.00 USD
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Product Specification

Alternative Name:Phosphoinositide-3-kinase
 
Clone:AB6
 
Host:Mouse
 
Isotype:IgG1
 
Immunogen:Recombinant human PI3 kinase (p85α).
 
UniProt ID:P27986
 
Species reactivity:Human, Mouse, Rat
 
Specificity:Recognizes the p85α subunit of PI3 kinase.
 
Applications:ICC, IP, WB
 
Recommended Dilutions/Conditions:Immunocytochemistry (10µg/ml)
Immunoprecipitation (5-10µg/ml per 100µg cell extract)
Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~85kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Phosphatidylinositol 3-kinase (PI3-K) is a lipid kinase that phosphorylates the inositol ring of phosphatidylinositol and related compounds at the 3
 
Regulatory Status:RUO - Research Use Only
 
PI3 kinase monoclonal antibody (AB6) Western blot
Western blot analysis of PI3 kinase expression in Jurkat (1), Raji (2), U937 (3), WR19L (4) and PC12 (5) using PI3-kinase, p85a mAb (AB6).
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PI3 kinase monoclonal antibody (AB6) Western blot

Product Literature References

A high-throughput pipeline for validation of antibodies: K. Sikorski, et al.; Nat. Methods 15, 909 (2018), Abstract;

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