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Bax monoclonal antibody (4F11)

ADI-AAM-140-E 100 µg 374.00 USD
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Product Details

Alternative Name:BCL2L4, BCL2-associated X protein
Immunogen:Recombinant human Bax.
UniProt ID:Q07812
Species reactivity:Human, Rat
Applications:IHC, IP, WB
Recommended Dilutions/Conditions:Immunohistochemistry (10µg/ml)
Immunoprecipitation (5-10µg/ml)
Western Blot (0.1-1.0µg/ml colorimetric, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~22kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Lyophilized. In PBS, pH 7.2, containing 1% sucrose. Reconstitute with 100μL of distilled water.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.
Regulatory Status:RUO - Research Use Only
Bax monoclonal antibody (4F11) Western blot
Western blot analysis of human Raji cell lysate, probed with Bax mAb (4F11)
Bax monoclonal antibody (4F11) Immunohistochemistry
Immunohistochemistry analysis of paraffin-embedded section of Alzheimer’s diseased (AD) brain, stained with Bax mAb (4F11).
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Bax monoclonal antibody (4F11) Western blot Bax monoclonal antibody (4F11) Immunohistochemistry

Product Literature References

Cdk5 phosphorylates p53 and regulates its activity: V.W. Johnson, et al. ; J. Neurochem. 81, 307 (2002), Application(s): WB using rat samples, Abstract;

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