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Direct 8-iso-PGF ELISA kit

Highly sensititive ELISA, measuring 8-iso-Prostaglandin F in plasma, serum and tissue.
ADI-901-091 5x96 wells 1,950.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-900-091 (1x96 wells)
  • Non-radioactive measurement of isoprostane in serum and plasma
  • Flexible options, either overnight or same day results
  • High sensitivity, measuring as little as 40 pg/ml
  • High throughput format with results for up to 39 samples in duplicate in less than 3 hours
  • Save with bulk kit package!
The measurement of F2-isoprostanes has emerged as one of the most reliable approaches to assess oxidative stress status in vivo, providing an important tool to explore the role of oxidative stress in the pathogenesis of human disease. The is a colorimetric competitive immunoassay kit for the quantitative determination of 8-iso-Prostaglandin F in biological fluids such as plasma, serum and tissue. To measure isoprostane in urine or culture supernatants, use the 8-iso-PGF EIA Kit.

8-iso-PGF is produced in vivo by both non-cyclooxygenase and cyclooxygenase dependant mechanisms from arachidonic acid. It has been shown to be a potent vasoconstrictor, a potential mediator of hepatorenal syndrome and a mutagen in 3T3 cells and vascular smooth muscle cells. 8-iso-PGF has been shown to circulate in plasma and is excreted in urine.

Product Details

Alternative Name:8-iso-Prostaglandin F2α
Sensitivity:103.2 pg/mL (<3 hour format), 40.0 pg/mL (overnight) (range 160 – 100,000 pg/mL)
Assay Time: <3 hours or Overnight + 45 min
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of 8-iso-PGF in plasma, serum, and tissue from any species. Cited sample types include cell lysate and urine.
Wavelength:405 nm
Species reactivity:Species independent
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:GxR Microtiter plate, Conjugate, Antibody, Sample diluent, Neutralizing reagent, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:Prostaglandin F (PGF) is formed in a variety of cells from PGH2, which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGF is often viewed as an antagonist to PGE2 due to their opposing effects on various tissues. PGF is a potent bronchoconstrictor and has been implicated in asthma attacks. PGF is also involved in reproductive functions including corpus luteum regulation, uterine contractions, and sperm motility. This has led to its use in terminating pregnancies and inducing labor at term. High levels of PGF have also been associated with preeclampsia.
Technical Info/Product Notes:Cited samples:
Wildlife Endocrinology: Cited Sample Types
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Coenzyme Q10 and Fish Oil Supplementation for Reducing Retinal Oxidative Stress in a Rat Model: F. Siddiqui, et al.; Vision (Basel) 7, 20 (2023), Abstract;
Effects of Chokeberries (Aronia spp.) on Cytoprotective and Cardiometabolic Markers and Semen Quality in 109 Mildly Hypercholesterolemic Danish Men: A Prospective, Double-Blinded, Randomized, Crossover Trial: J. Sangild, et al.; J. Clin. Med. 12, 373 (2023), Abstract;
Combustion-Derived Particulate PAHs Associated with Small Airway Dysfunction in Elderly Patients with COPD: T. Wang, et al.; Environ. Sci. Technol. 56, 10868 (2022), Abstract;
Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage: K.M. Mirabito Colafella, et al.; Clin. Sci. (Lond.) 136, 675 (2022), Abstract;
Infection of Galleria mellonella (lepidoptera) larvae with the entomopathogenic fungus Conidiobolus coronatus (entomophthorales) induces apoptosis of hemocytes and affects the concentration of eicosanoids in the hemolymph: A.K. Wronska, et al.; Front. Physiol. 12, 774086 (2022), Application(s): ELISA kit using hemolymph, Abstract; Full Text
Lipid peroxidation products as predictors of oxidant-mediated disease in preterm infants: K. Katti, et al.; J. Matern. Fetal Neonatal Med. 35, 4878 (2022), Abstract;
Antioxidants and/or fish oil reduce intermittent hypoxia-induced inflammation in the neonatal rat terminal ileum: G. Mustafa, et al.; Prostaglandins Other Lipid Mediat. 155, 106565 (2021), Abstract;
Comparison of coenzyme Q10 or fish oil for prevention of intermittent hypoxia-induced oxidative injury in neonatal rat lungs: C. D'Agrosa, et al.; Respir. Res. 22, 196 (2021), Abstract;
Dose Response of Bumetanide on Aquaporins and Angiogenesis Biomarkers in Human Retinal Endothelial Cells Exposed to Intermittent Hypoxia: S. Guzel, et al.; Pharmaceuticals 14, 967 (2021), Abstract;
Relation of moderate physical activity to blood markers of oxidative stress and antioxidant defense in the elderly: M. Kozakiewicz, et al.; Oxid. Med. Cell. Longev. 2019, 5123628 (2019), Application(s): ELISA using human plasma, Abstract; Full Text
Antifibrogenic effect of melatonin in rats with experimental liver cirrhosis induced by carbon tetrachloride: S. Bona, et al.; JGH Open 2, 117 (2018), Application(s): ELISA using rat liver homogenates, Abstract; Full Text
Oxidative stress is a convincing contributor to idiopathic chronic fatigue: J.S. Lee, et al.; Sci. Rep. 8, 12890 (2018), Application(s): ELISA using human serum, Abstract; Full Text
Can allopurinol improve retinopathy in diabetic rats? Oxidative stress or uric acid; which one is the culprit?: M. Goharinia, et al.; Res. Pharm. Sci. 12, 401 (2017), Application(s): Plasma Samples, Abstract; Full Text
Lycopene and tomato powder supplementation similarly inhibit high-fat diet induced obesity, inflammatory response, and associated metabolic disorders: S. Fenni, et al.; Mol. Nutr. Food Res. 61, (2017), Application(s): Mouse Serum, Abstract;
Placental antioxidant enzyme status and lipid peroxidation in pregnant women with type 1 diabetes: the effect of vitamin C and E supplementation : P.C. Johnston, et al.; J. Diabetes Complications 30, 109 (2016), Application(s): Measurement of placental 8-iso-Prostaglandin F2α , Abstract;
Quercetin feeding in newborn dairy calves cannot compensate colostrum deprivation: Study on metabolic, antioxidative and inflammatory traits: J. Gruse, et al.; PLoS One 11, e0146932 (2016), Application(s): ELISA using bovine plasma, Abstract; Full Text
The activity of serum 8-iso-prostaglandin F2a as oxidative stress marker in patients with diabetes mellitus type 2 and associated dyslipidemic hyperglycemia: M.H. Mukhtar, et al.; J. Diab. Mellitus 6, 318 (2016), Application(s): ELISA using human serum samples, Full Text
A randomized clinical trial of ascorbic acid in open abdominal aortic aneurysm repair: M.J. Duffy, et al.; Intensive Care Med. Exp. 3, 20 (2015), Application(s): ELISA using human plasma, Abstract; Full Text
CYBA (p22phox) variants associate with blood pressure and oxidative stress markers in hypertension: a replication study in populations of diverse altitudes: R. Kumar, et al.; Hypertens. Res. 38, 498 (2015), Application(s): ELISA using human plasma, Abstract;
Mitochondrial translocation of human telomerase reverse transcriptase in cord blood mononuclear cells of newborns with gestational diabetes mellitus mothers: P. Li, et al.; Diabetes Res. Clin. Pract. 103, 310 (2014), Application(s): ELISA using human plasma, Abstract;
Comparison of serum F2 isoprostane levels in diabetic patients and diabetic patients infected with Burkholderia pseudomallei: S. Puthucheary, et al. ; Singapore Med. J. 49, 117 (2008), Application(s): EIA using human serum, Abstract;
Lipid peroxidation products do not activate hepatic stellate cells: W. Lin, et al. ; Toxicology 253, 36 (2008), Application(s): EIA using rat tissue, Abstract;
Cigarette smokers have decreased lymphocyte and platelet alpha-tocopherol levels and increased excretion of the gamma-tocopherol metabolite gamma-carboxyethyl-hydroxychroman (gamma-CEHC): Y.M. Jeanes, et al. ; Free Radic. Res. 38, 861 (2004), Application(s): EIA using human plasma, Abstract;
Differential oxidant potential of carcinogenic and weakly carcinogenic estrogens: Involvement of metabolic activation and cytochrome P450: M.M. Patel, et al. ; J. Biochem. Mol. Toxicol. 18, 37 (2004), Application(s): EIA using human & hamster cell lysates, Abstract;
Critical role of oxidative stress in estrogen-induced carcinogenesis: H.K. Bhat, et al. ; PNAS 100, 3913 (2003), Application(s): EIA using hamster cell lysates, Abstract;
Treadmill exercise in mice increases intestinal lymphocyte loss via apoptosis: L. Hoffman-Goetz, et al. ; Acta Physiol. Scand. 179, 289 (2003), Application(s): EIA using mouse plasma, Abstract;
Effects of vitamin C and vitamin E on in vivo lipid peroxidation: results of a randomized controlled trial: H.Y. Huang, et al. ; Am. J. Clin. Nutr. 76, 549 (2002), Application(s): EIA using human urine, Abstract;
Oxidant stress in sled dogs subjected to repetitive endurance exercise: K.W. Hinchcliff, et al. ; Am. J. Vet. Res. 61, 512 (2000), Application(s): EIA using canine plasma, Abstract;

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