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Cortisol ELISA kit

Widely used sensitive cortisol ELISA kit with a large variety of sample types and highly reproducible results for stress, autoimmune disease and cancer research.
ADI-901-071 5x96 wells 1,538.00 USD
Do you need bulk/larger quantities?
Alternative size available: ADI-900-071 (1x96 wells)
  • Senstitive measurement of cortisol, detecting as little as 56.72 pg/ml
  • Highly cited in peer reviewed literature
  • Reproducible results day-after-day and lot-after-lot
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
  • Easy-to-use pre-coated plates and liquid color-coded reagents save time and reduce errors
  • Save with bulk kit package!
The Cortisol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. This kit is highly cited in peer-reviewed publications. The ready-to-use liquid color-coded reagents provided are safe, non-radioactive, and reduce error in the lab. The broad dynamic range lets you accurately measure Cortisol levels in a variety of sample matrices.
Cortisol ELISA kit Standard curve
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Cortisol ELISA kit Standard curve

Product Details

Sensitivity:56.72 pg/ml (range 156 - 10,000 pg/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of Cortisol in culture supernatants, plasma, serum, saliva, and urine from any species. Cited sample type includes feces.
Wavelength:405 nm
Species reactivity:Species independent
Crossreactivity:Cortisol (100%), Prednisolone (122.35%), Corticosterone (27.68%), 11-deoxycortisol (4.0%), Progesterone (3.64%), Prednisone (0.85%), Testosterone (0.12%) and <0.10%: Androstenedione, Cortisone, Estradiol
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Contents:GxM Microtiter plate, Conjugate, Antibody, Assay buffer concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent
Scientific Background:Cortisol (hydrocortisone, compound F) is a steroid hormone synthesized from cholesterol. It is the primary glucocorticoid produced and secreted by the adrenal cortex. Cortisol is found in the blood either as free cortisol, or bound to corticosteroid-binding globulin (CBG). Serum levels are highest in the early morning and decrease throughout the day. Cortisol is known to regulate metabolism, promoting gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization. Cortisol production is positively correlated with stress, resulting in increased blood pressure, and exerts immunosuppressive effects on the immune system.
Technical Info/Product Notes:Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

Age-dependent effects of embryonic ethanol exposure on anxiety-like behaviours in young zebrafish: A genotype comparison study: A. Facciol, et al.; Pharmacol. Biochem. Behav. 214, 173342 (2022), Abstract;
Caregiving of children with Down syndrome: impact on quality of life, stress, mental and oral health: C. Dias, et al.; Spec. Care Dentist. (2022), Abstract;
Loss of an apex predator in the wild induces physiological and behavioural changes in prey: N. Hammerschlag, et al.; Biol. Lett. 18, 20210476 (2022), Abstract;
Novel regulation mechanism of adrenal cortisol and DHEA biosynthesis via the endogen ERAD inhibitor small VCP-interacting protein: R. Iihan, et al.; Sci. Rep. 12, 869 (2022), Abstract;
Application of Creep Feed and Phytase Super-Dosing as Tools to Support Digestive Adaption and Feed Efficiency in Piglets at Weaning: S.A. Lee, et al.; Animals (Basel) 11, 2080 (2021), Abstract;
Association of gastrointestinal parasite burden, serum cytokines and hormones concentrations, and pregnancy in Angus-cross beef cows: R.K. Kasimanickam, et al.; Vet. Parasitol. 295, 109464 (2021), Abstract;
Effect of handling and crowding on the susceptibility of Atlantic salmon (Salmo salar L.) to Lepeophtheirus salmonis (Krøyer) copepodids: C. Delfosse, et al.; J. Fish Dis. 44, 327 (2021), Abstract; Full Text
Functional neuroanatomy and neural oscillations during social eavesdropping in male golden hamsters: C. Liu, et al.; Horm. Behav. 127, 104881 (2021), Application(s): ELISA using hamster serum, Abstract;
Glucocorticoids and the cytokines IL-12, IL-15, and IL-18 present in the tumor microenvironment induce PD-1 expression on human natural killer cells: L. Quatrini, et al.; J. Allergy Clin. Immunol. 147, 349 (2021), Application(s): ELISA using human plasma and pleural effusion, Abstract;
Hormonal and Neurological Aspects of Dog Walking for Dog Owners and Pet Dogs: J. Akiyama & M. Ohta; Animals 11, 2732 (2021), Abstract;
Impact of heat stress on embryonic development during first 16 days of gestation in dairy cows: R. Kasimanickam & V. Kasimanickam; Sci. Rep. 11, 14839 (2021), Abstract;
Omentin-1 attenuates glucocorticoid-induced cardiac injury by phosphorylating GSK3β: Z. Jin, et al.; J. Mol. Endocrinol. 66, 273 (2021), Application(s): ELISA using human plasma, Abstract;
Response of esophageal epithelium to acute and chronic stress in rabbits: P. Ergün, et al.; Bull. Exp. Biol. Med. 171, 582 (2021), Application(s): ELISA using rabbit plasma, Abstract;
The Effects of Beverage Intake after Exhaustive Exercise on Organ Damage, Inflammation and Oxidative Stress in Healthy Males: T. Tominaga, et al.; Antioxidants (Basel) 10, 866 (2021), Application(s): Cortisol quantification on human urine and plasma samples, Abstract; Full Text
Does Maternal Stress Affect the Early Embryonic Microenvironment? Impact of Long-Term Cortisol Stimulation on the Oviduct Epithelium: S. Du, et al.; Int. J. Mol. Sci. 21, 443 (2020), Abstract; Full Text
Effects of Zinc Oxide and Arginine on the Intestinal Microbiota and Immune Status of Weaned Pigs Subjected to High Ambient Temperature: S.Y. Yoon, et al.; Animals (Basel) 10, 1537 (2020), Abstract; Full Text
So-Ochim-Tang-Gamibang, a Traditional Herbal Formula, Ameliorates Depression by Regulating Hyperactive Glucocorticoid Signaling In Vitro and In Vivo: M. Jin, et al.; Evid. Based Complement. Alternat. Med. 2020, 8834556 (2020), Abstract; Full Text
Cardiac autonomic activity during simulated shift work: E. Skornyakov, et al.; Ind. Health 57, 118 (2019), Application(s): ELISA using human plasma, Abstract; Full Text
Effects of trichlorfon on oxidative stress, neurotoxicity, and cortisol levels in common carp, Cyprinus carpio L., at different temperatures: S.J. Woo, et al.; Comp. Biochem. Physiol. C Toxicol. Pharmacol. 229, 108698 (2019), Abstract;
Stress-induced changes in group behaviour: T.K. Kleinhappel, et al.; Sci. Rep. 9, 17200 (2019), Abstract; Full Text
The study of ameliorative effect of dietary supplementation of vitamin C, vitamin E, and tryptophan on Labeo rohita (Cyprinidae) fry exposed to intense light: N.A. Khan, et al.; Fish. Physiol. Biochem. 45, 1153 (2019), Application(s): ELISA using fish serum, Abstract;
Transient exposure of methylparaben to zebrafish (Danio rerio) embryos altered cortisol level, acetylcholinesterase activity and induced anxiety-like behaviour: G. Raja, et al.; Gen. Comp. Endocrinol. 279, 53 (2019), Abstract;
Baleen whale cortisol levels reveal a physiological response to 20th century whaling: S.J. Trumble, et al.; Nat. Commun. 9, 4587 (2018), Abstract; Full Text
Disaggregation of adrenocorticotropic hormone and cortisol levels in HIV‐positive, methamphetamine‐using men who have sex with men: A.W. Carrico, et al.; Hum. Psychopharmacol. 33, e2645 (2018), Application(s): ELISA using human serum, Abstract; Full Text
Extra-adrenal glucocorticoids contribute to the postprandial increase of circulating leptin in mice: Y. Tomabechi, et al.; J. Cell. Commun. Signal. 12, 433 (2018), Application(s): ELISA using culture supernatants, Abstract; Full Text
Involuntary swimming exercise in pregnant rats disturbs ERK1/2 signaling in embryonic neurons through increased cortisol in the amniotic fluid: Y. Jang, et al.; Biochem. Biophys. Res. Commun. 495, 1208 (2018), Abstract;
Leucine and glycine dipeptides of porcine placenta ameliorate physical fatigue through enhancing dopaminergic systems: N.R. Han, et al.; Mol. Med. Rep. 17, 4120 (2018), Application(s): ELISA using mouse serum, Abstract;
Persistent Zika virus infection in porcine conceptuses is associated with elevated in utero cortisol levels: I. Trus, et al.; Virulence 9, 1338 (2018), Application(s): ELISA using porcine amniotic fluid and plasma, Abstract;
The effects of continuously acoustical stress on cortisol in milkfish (Chanos chanos): C.A. Wei, et al.; Gen. Comp. Endocrinol. 257, 227 (2018), Application(s): ELISA using fish plasma, Abstract;
Tryptophan metabolic profile in term and preterm breast milk: implications for health: L. O'Rourke, et al.; J. Nutr. Sci. 7, e13 (2018), Application(s): Cort levels w/ Human Expressed Breast Milk (EMB), Abstract; Full Text
Brain and Hepatic Mt mRNA Is Reduced in Response to Mild Energy Restriction and n-3 Polyunsaturated Fatty Acid Deficiency in Juvenile Rats: A.A. Mehus, et al.; Nutrients 9, E1145 (2017), Application(s): Rat plasma, Abstract; Full Text
Consumption of green tea epigallocatechin-3-gallate enhances systemic immune response, antioxidative capacity and HPA axis functions in aged male swiss albino mice: R. Sharma, et al.; Biogerontology 18, 367 (2017), Application(s): Mouse plasma, Abstract;
Glucose and insulin modulate sickness responses in male Siberian hamsters: E.D. Carlton & G.E. Demas; Gen. Comp. Endocrinol. 242, 83 (2017), Application(s): Cortisol levels measured in hamster serum, Abstract;
Leukocyte profile, gene expression, acute phase response, and metabolite status of cows with sole hemorrhages: K. O'Driscoll, et al.; J. Dairy Sci. 100, 11 (2017), Application(s): Bovine Serum, Abstract;
Perch, Perca fluviatilis show a directional preference for, but do not increase attacks toward, prey in response to water-borne cortisol: L.J. Henderson, et al.; PeerJ. 5, e3883 (2017), Application(s): Water samples, Abstract;
Peripheral regulation of stress and fear responses in pigs from tail-biting pens: M. Zupan, et al.; R. Bras. Zootec. 46, 33 (2017), Application(s): Measurement of cortisol in pig saliva samples, Full Text
Relocation stress induces short-term fecal cortisol increase in Tonkean macaques (Macaca tonkeana): C. Cinque, et al.; Primates 58, 315 (2017), Application(s): Measurement of fecal cortisol metabolite concentrations in Tonkean macaques, Abstract;
Stress and immunological response of heifers divergently ranked for residual feed intake following an adrenocorticotropic hormone challenge: A.K. Kelly, et al.; J. Anim. Sci. Biotechnol. 8, 65 (2017), Application(s): Beef heifer plasma samples, Abstract; Full Text
The Sheep Grimace Scale as an indicator of post-operative distress and pain in laboratory sheep: C. Häger, et al.; PLoS One 12, e0175839 (2017), Abstract; Full Text
Toxic effects of juvenile sablefish, Anoplopoma fimbria by ammonia exposure at different water temperature: J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 54, 169 (2017), Application(s): Fish plasma from Sablefish (Anoplopoma fimbria), Abstract;
Air pollution, ethnicity and telomere length in east London schoolchildren: An observational study: R.T. Walton, et al.; Environ. Int. 96, 41 (2016), Abstract;
Bifidobacterium longum 1714 as a translational psychobiotic: modulation of stress, electrophysiology and neurocognition in healthy volunteers: A.P. Allen, et al.; Transl. Psychiatry 6, e939 (2016), Application(s): Salivary samples, Abstract;
Effects of turmeric curcuminoids and metformin against central sensitivity to pain in mice: S. Verma, et al.; J. Tradit. Complement. Med. 7, 145 (2016), Application(s): Mouse tail plasma cortisol levels were measured, Abstract; Full Text
Efficacy of oral meloxicam suspension for prevention of pain and inflammation following band and surgical castration in calves: M. Olson, et al.; BMC Vet. Res. 12, 102 (2016), Application(s): ELISA using blood samples, Abstract; Full Text
Expansion of bone marrow adipose tissue during caloric restriction is associated with increased circulating glucocorticoids and not with hypoleptinemia: W.P. Cawthorn, et al.; Endocrinology 157, 508 (2016), Application(s): Determination of cortisol levels in blood and serum, Abstract; Full Text
Expression and regulation of 11β-hydroxysteroid dehydrogenase type 1 in first trimester human decidua cells: Implication in preeclampsia: L. Funghi, et al.; Mol. Cell. Endocrinol. 437, 163 (2016), Application(s): Enzymatic activity, cell homogenates, Abstract;
Intermittent Suckling Causes a Transient Increase in Cortisol That Does Not Appear to Compromise Selected Measures of Piglet Welfare and Stress: D.L. Turpin, et al.; Animals (Basel) 6, e24 (2016), Application(s): Measured plasma cortisol levels, Abstract; Full Text
Intermittent Suckling in Combination with an Older Weaning Age Improves Growth, Feed Intake and Aspects of Gastrointestinal Tract Carbohydrate Absorption in Pigs after Weaning: D.L. Turpin, et al.; Animals (Basel) 6, e66 (2016), Application(s): Analysis of cortisol in piglet plasma, Abstract; Full Text
Oxysterol-related-binding-protein related Protein-2 (ORP2) regulates cortisol biosynthesis and cholesterol homeostasis: T. Escajadillo, et al.; Mol. Cell. Endrocrinol. 427, 73 (2016), Application(s): Measured cortisol levels, Abstract;
Physiological and psychological responses in Fire Instructors to heat exposures: P.W. Watt, et al.; J. Therm. Biol. 58, 106 (2016), Application(s): Cortisol levels were measured in plasma samples, Abstract;
Psychological Profiles in the Prediction of Leukocyte Telomere Length in Healthy Individuals: L. Starnino, et al.; PLoS One 11, e0165482 (2016), Application(s): Measuring human salivary cortisol levels, Abstract; Full Text
Stress in the neighborhood: Tissue glucocorticoids relative to stream quality for five species of fish: G.D. King, et al.; Sci. Total Environ. 547, 87 (2016), Application(s): Cortisol levels measured in fish tissue, Abstract;
The immune responses and expression of metallothionein (MT) gene and heat shock protein 70 (HSP 70) in juvenile rockfish, Sebastes schlegelii, exposed to waterborne arsenic (As3+): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 47, 136 (2016), Application(s): Measurement of plasma cortisol concentration, Abstract;
The immune responses in juvenile rockfish, Sebastes schlegelii for the stress by the exposure to the dietary lead (II): J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 46, 211 (2016), Application(s): Plasma cortisol concentration measurement, Abstract;
The toxic effects on the stress and immune responses in juvenile rockfish, Sebastes schlegelii exposed to hexavalent chromium: J.H. Kim, et al.; Environ. Toxicol. Pharmacol. 43, 128 (2016), Application(s): Measurement of plasma cortisol concentration in rockfish, Abstract;
Using physiological and zootechnical profiles to evaluate welfare in farmed rainbow trout Oncorhynchus mykiss (Walbaum) under stressful conditions: C. Delfosse, et al.; Aquacult. Int. 24, 1449 (2016), Application(s): Cortisol levels in trout plasma, Abstract;
A potent and selective 11β-hydroxysteroid dehydrogenase type 1 inhibitor, SKI2852, ameliorates metabolic syndrome in diabetic mice models: H. Oh, et al.; Eur. J. Pharmacol. (2015), Application(s): Ex vivo analysis in mice tissue, Abstract;
Decreased Cortisol and Pain in Breast Cancer: Biofield Therapy Potential: A. Running; Evid. Based Complement. Alternat. Med. 2015, 870640 (2015), Application(s): ELISA using mouse feces, Abstract; Full Text
Differences in leukocyte profile, gene expression, and metabolite status of dairy cows with or without sole ulcers: K. O'Driscoll, et al.; J. Dairy Sci. 98, 1685 (2015), Application(s): Bovine Serum, Abstract;
Effect of floor type on the performance, physiological and behavioural responses of finishing beef steers: B. Earley, et al.; Acta. Vet. Scand. 57, 73 (2015), Application(s): Quantitative determination of cortisol in plasma, Abstract; Full Text
Effects of Oxytocin Administration on the Response of Piglets to Weaning: J.L. Rault, et al.; Animals (Basel) 5, 545 (2015), Application(s): ELISA using pig plasma, Abstract; Full Text
Identification of a novel 11β-HSD1 inhibitor from a high-throughput screen of natural product extracts: S.B. Park, et al.; Pharmacol. Res. 102, 245 (2015), Application(s): Ex vivo assay for 11β-HSD1 activity, Abstract;
Mares prefer the voices of highly fertile stallions: A. Lemasson, et al.; PLoS One 10, e0118468 (2015), Application(s): ELISA using equine plasma, Abstract; Full Text
Blow Collection as a Non-Invasive Method for Measuring Cortisol in the Beluga (Delphinapterus leucas): L.A. Thompson, et al.; PLoS One 9, e114062 (2014), Abstract; Full Text
A test of maternal programming of offspring stress response to predation risk in threespine sticklebacks: B.C. Mommer, et al.; Physiol. Behav. 122, 222 (2013), Application(s): EIA using fish plasma, Abstract;
Glucocorticoid stress responses of lions in relationship to group composition, human land use, and proximity to people: S. Creel, et al.; Conserv. Physiol. 1, cot021 (2013), Abstract; Full Text
Sex-specific interaction effects of age, occupational status, and workplace stress on psychiatric symptoms and allostatic load among healthy Montreal workers: R.P. Juster, et al.; Stress 16, 616 (2013), Application(s): Human Saliva using salivette sampling, Abstract;
Spatial and temporal influences on the physiological condition of invasive silver carp: S.A. Liss, et al.; Conserv. Physiol. 11, cot017 (2013), Abstract; Full Text
Sphingosine-1-phosphate rapidly increases cortisol biosynthesis and the expression of genes involved in cholesterol uptake and transport in H295R adrenocortical cells: N.C. Lucki, et al.; Mol. Cell Endocrinol. 348, 165 (2012), Application(s): EIA using cell culture supernatants, Abstract; Full Text
Unravelling complex associations between testosterone and parasite infection in the wild: V.O. Ezenwa, et al.; Functional Ecology 26, 123 (2012), Application(s): ELISA using gazelle feces, Abstract;
Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress and energetic metabolism biomarkers in urine specimens: K.G. Salvante, et al.; Am. J. Hum. Biol. 24, 81 (2012), Application(s): Human urine, Abstract; Full Text
Comparison of evaporation techniques for the preparation of salivary cortisol for analysis by liquid chromatography-electrospray tandem mass spectrometry: E. Nelson, et al. ; Clin. Biochem. 41, 1413 (2008), Application(s): EIA using human saliva, Abstract;
Expression of arginine vasotocin in distinct preoptic regions is associated with dominant and subordinate behaviour in an African cichlid fish: R. Fernald, et al. ; Proc. Biol. Sci. 275, 2393 (2008), Application(s): EIA using fish plasma, Abstract;
Stress assessment in acute care department nurses by measuring interleukin-8: H. Fukuda, et al. ; Int. Nurs. Rev. 55, 407 (2008), Application(s): EIA using human urine, Abstract;
Attenuation of the glucocorticoid response during Ad5IL-12 adenovirus vector treatment enhances natural killer cell-mediated killing of MHCclass I-negative LNCaP prostate tumors: T. Braciak, et al. ; Cancer Res. 67, 2290 (2007), Application(s): EIA using mouse serum, Abstract;
Adrenal hormones mediate melatonin-induced increases in aggression in male Siberian hamsters (Phodopus sungorus): G.E. Demas, et al.; Horm. Behav. 46, 582 (2004), Application(s): Blood serum testosterone and serum cortisol from Siberian hamsters, Abstract;
Behavioural and neuroendocrine adaptations to repeated stress during puberty in male golden hamsters: J.C. Wommack, et al. ; J. Neuroendocrinol. 16, 767 (2004), Application(s): EIA using hamster plasma, Abstract;
Repeated social stress and the development of agonistic behavior: individual differences in coping responses in male golden hamsters: J.C. Wommack, et al. ; Physiol. Behav. 80, 303 (2003), Application(s): EIA using hamster serum, Abstract;

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