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Serotonin ELISA kit

Easy-to-use, well-validated serotonin ELISA kit for hormone research.
ADI-900-175 96 wells 693.00 USD
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  • Highly sensitive measurement of serotonin, detecting as little as 0.293 ng/ml
  • No acylation required for standards and samples
  • Thoroughly validated in multiple complex sample matrices
  • High throughput format with results in just 3 hours for up to 39 samples in duplicate
  • Fully quantitative results that surpass semi-quantitiave Western blot analysis
The Serotonin EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours.
Serotonin ELISA kit Standard curve
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Serotonin ELISA kit Standard curve

Product Details

Alternative Name:5-HT, 5-hydroxytryptamine
Sensitivity:0.293 ng/ml (range 0.49 - 500 ng/ml)
Assay Time:3 hours
Applications:ELISA, Colorimetric detection
Application Notes:For the quantitative determination of Serotonin in platelets, plasma, serum, and urine from any species. Cited sample type includes culture supernatant.
Wavelength:405 nm
Species reactivity:Species independent
Crossreactivity:N-acetyl serotonin (17%), 5-hydroxy-L-tryptophan (0.4%), Tryptamine (0.1%), 5-hydroxyindoleacetic acid (0.03%), Melatonin (0.01%), Tyramine (<0.004%), Tryptophan (<0.004%)
Use/Stability:Store all components at -20°C
Shipping:Blue Ice
Contents:GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution
Scientific Background:Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine found in the central nervous system, gastrointestinal tract, and blood with broad physiological functions in neurotransmission, gastric motility, hemostasis, and cardiovascular integrity. Seven subclasses (5-HT 1-7) of serotonin receptors have been identified, a majority of which are members of the seven transmembrane domain-containing GPCR family, and are the target of several anti-depressant drugs.  The 5-HT1 subclass consists of five highly homologous receptors that couple via G(i/o) alpha subunits to inhibit adenylyl cyclase activity. 5-HT1A expression in mammals is strongest in CNS limbic pathways that control emotion, including tissue of the hippocampus, dorsal raphé, and septum. Serotonin is a key biomarker used for the monitoring of overall welfare and/or the assessment of behavioral disorders in a variety of species.
Technical Info/Product Notes:Application Note:
Validation of a Serotonin ELISA Kit with Blood Samples from Three Domestic Animal Species.

Cited samples:
Hormone ELISAs: Cited Samples
Regulatory Status:RUO - Research Use Only
Compatibility:This product is compatible with the Absorbance 96 Plate Reader.

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Product Literature References

SSRI treatment modifies the effects of maternal inflammation on in utero physiology and offspring neurobiology: K.E. Zengeler, et al.; Brain Behav. Immun. Health 108, 80 (2023), Abstract;
Associations of neurotransmitters and the gut microbiome with emotional distress in mixed type of irritable bowel syndrome: Z.A. Barandouzi, et al.; Sci. Rep. 12, 1648 (2022), Abstract;
Enhanced presence of serotonin in nasal cavity after autologous stimulation: F. Salvinelli, et al.; Eur. Rev. Med. Pharmacol. Sci. 256, 2970 (2022), Abstract;
Gut bacterial aromatic amine production: aromatic amino acid decarboxylase and its effects on peripheral serotonin production: Y. Sugiyama, et al.; Gut Microbes 14, 2128605 (2022), Abstract;
Improvement of several stress response and sleep quality hormones in men and women after sleeping in a bed that protects against electromagnetic fields: E.D.D. Cerro, et al.; Environ. Health 21, 72 (2022), Abstract;
Modulation of Intestinal Corticotropin-Releasing Hormone Signaling by the Herbal Preparation STW 5-II: Possible Mechanisms for Irritable Bowel Syndrome Management: M. Elbadawi, et al.; Pharmaceuticals (Basel) 15, 1121 (2022), Abstract;
Phytochemical screening and effect of Viscum album L. on monoamine oxidase A and B activity and serotonin, dopamine and serotonin receptor 5-HTR1A levels in Galleria mellonealla (Lepidoptera): A. Szurpnicka, et al.; J. Ethnopharmacol. 298, 115604 (2022), Abstract;
Telotristat ethyl reverses myxomatous changes in mice mitral valves: X. Wang, et al.; Front. Cardiovasc. Med. 9, 945672 (2022), Abstract;
Airway basal stem cells generate distinct subpopulations of PNECs: H. Mou, et al.; Cell Rep. 35, 109011 (2021), Abstract;
Analysis of cytokine levels, T regulatory cells and serotonin content in patients with depression: J. Obermanns, et al.; Eur. J. Neurosci. 53, 3476 (2021), Application(s): ELISA using human serum, Abstract;
Neuroprotective role of camphor against ciprofloxacin induced depression in rats: modulation of Nrf-2 and TLR4: A. Salama, et al.; Immunopharmacol. Immunotoxicol. 43, 309 (2021), Abstract;
Sensing serotonin secreted from human serotonergic neurons using aptamer-modified nanopipettes: N. Nakatsuka, et al.; Mol. Psychiatry 26, 2753 (2021), Abstract;
Serotonin involvement in okadaic acid-induced diarrhoea in vivo: M.C. Louzao, et al.; Arch. Toxicol. 95, 2797 (2021), Abstract;
Coagulation Profiles of Pulmonary Arterial Hypertension Patients, Assessed by Non-Conventional Hemostatic Tests and Markers of Platelet Activation and Endothelial Dysfunction: E. Vrigkou, et al.; Diagnostics (Basel) 10, 758 (2020), Abstract;
Effects on tactile transmission by serotonin transporter inhibitors at Merkel discs of mouse whisker hair follicles: W. Chang & J.G. Gu; Mol. Pain 16, 1744806920938237 (2020), Abstract; Full Text
Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood: E. Mackey, et al.; PNAS 117, 23751 (2020), Application(s): Lysed BMMCs (bone marrow-derived Mast Cells) supernatants, Abstract;
Platelet inhibition by ticagrelor is protective against diabetic nephropathy in mice: M. Uil, et al.; FASEB J. 34, 13750 (2020), Application(s): Mouse plasma, Abstract; Full Text
Unexpected effects of long-term treatment with acetylsalicylic acid on late phase of pulmonary metastasis in murine model of orthotopic breast cancer: M. Smeda, et al.; PLoS One 15, e0230520 (2020), Abstract; Full Text
A prospective study of the pathophysiology of carcinoid crisis: M.E. Condron, et al.; Surgery 165, 158 (2019), Application(s): ELISA using human serum, Abstract;
Effects of anisomycin infusions into the dorsal striatum on memory consolidation of intense training and neurotransmitter activity: D.A. Gonzalez-Franco, et al.; Brain Res. Bull. 150, 250 (2019), Application(s): ELISA using rat dorsal striatum, Abstract;
Nppb neurons are sensors of mast cell-induced itch: H.J. Solinski, et al.; Cell Rep. 26, 3561 (2019), Application(s): ELISA using culture supernatant, Abstract; Full Text
Platelet-Dense Granules Worsen Pre-Infection Thrombocytopenia during Gram-Negative Pneumonia-Derived Sepsis: T.A.M. Claushuis, et al.; J. Innate Immun. 11, 168 (2019), Abstract; Full Text
Potential Antimigraine Effects of Warfarin: An Exploration of Biological Mechanism with Survey of Patients: B. Nilsson, et al.; TH Open 3, e180 (2019), Abstract; Full Text
Prolongation of bronchopulmonary C-fiber–mediated apnea by prenatal nicotinic exposure in rat pups: role of 5-HT3 receptors: L. Zhao, et al.; FASEB J. 33, 10731 (2019), Application(s): ELISA using rat BALF, Abstract;
Selective Serotonin Reuptake Inhibitors Aggravate Depression-Associated Dry Eye Via Activating the NF-κB Pathway: X. Zhang, et al.; Invest. Ophthalmol. Vis. Sci. 60, 419 (2019), Application(s): Serotonin analysis on tear fluid, Abstract; Full Text
Carbon Dioxide and Nitrogen Infused Compressed Air Foam for Depopulation of Caged Laying Hens: S. Gurung, et al.; Animals (Basel) 8, E6 (2018), Abstract; Full Text
Dose-response relationship of tryptophan with large neutral amino acids, and its impact on physiological responses in the chick model: A.U. Bello, et al.; Gen. Comp. Endocrinol. 260, 146 (2018), Abstract;
Evaluation of alternative euthanasia methods of neonatal chickens: S. Gurung, et al.; Animals (Basel) 8, 37 (2018), Application(s): ELISA using chicken serum, Abstract; Full Text
Fecal Microbiota Transplantation Can Alleviate Gastrointestinal Transit in Rats with High-Fat Diet-Induced Obesity via Regulation of Serotonin Biosynthesis: W. Sun, et al.; Biomed. Res. Int. 2018, 8308671 (2018), Abstract; Full Text
Hashimoto's thyroiditis induces neuroinflammation and emotional alterations in euthyroid mice: Y. J. Cai, et al.; J. Neuroinflammation 15, 299 (2018), Abstract; Full Text
Influence of Xiaoyaosan on depressive-like behaviors in chronic stress-depressed rats through regulating tryptophan metabolism in hippocampus: H. Jiao, et al.; Neuropsychiatr. Dis. Treat. 15, 21 (2018), Application(s): ELISA using rat hippocampus, Abstract; Full Text
Linalool ameliorates memory loss and behavioral impairment induced by REM-sleep deprivation through the serotonergic pathway: B.K. Lee, et al.; Biomol. Ther. 26, 368 (2018), Application(s): Serotonin levels in mouse brain, Abstract; Full Text
Long-Term Diet Supplementation with Lactobacillus paracasei K71 Prevents Age-Related Cognitive Decline in Senescence-Accelerated Mouse Prone 8: H.M. Corpuz, et al.; Nutrients 10, E762 (2018), Abstract; Full Text
Peripheral serotonin receptor 2B and transient receptor potential channel 4 mediate pruritus to serotonergic antidepressants in mice: S.H. Lee, et al.; J. Allergy Clin. Immunol. 142, 1349 (2018), Application(s): ELISA using mouse cheek skin, Abstract;
Platelet Toll-like receptor expression and activation induced by lipopolysaccharide and sepsis: T.A.M. Claushuis, et al.; Platelets 12, 1 (2018), Abstract; Full Text
Providing straw to allow exploratory behaviour in a pig experimental system does not modify putative indicators of positive welfare: peripheral oxytocin and serotonin: M. Marcet Rius, et al.; Animal 12, 2138 (2018), Application(s): Serotonin levels in pig serotonin, Abstract;
Serotonin induced hepatic steatosis is associated with modulation of autophagy and notch signaling pathway: S. Niture, et al.; Cell Commun. Signal. 16, 78 (2018), Abstract; Full Text
Use of calcitriol to maintain postpartum blood calcium and improve immune function in dairy cows: A. Vieira-Neto, et al.; J. Dairy Sci. 100, 5805 (2018), Application(s): Cow blood samples, Abstract;
Activation of the ileal neuroendocrine tumor cell line P-STS by acetylcholine is amplified by histamine: role of H3R and H4R: B. Pfanzagl, et al.; Sci. Rep. 7, 1313 (2017), Abstract; Full Text
Association between salivary serotonin and the social sharing of happiness: M. Matsunaga, et al.; PLoS One 12, e0180391 (2017), Abstract; Full Text
Brain injury induces specific changes in the caecal microbiota of mice via altered autonomic activity and mucoprotein production: A. Houlden, et al.; Brain. Behav. Immun. 57, 10 (2016), Application(s): Mouse tissue serotonin levels were measured, Abstract;
Cardiac volume overload and pulmonary hypertension in long-term follow-up of patients with a transjugular intrahepatic portosystemic shunt: A. Wannhoff, et al.; Aliment. Pharmacol. Ther. 43, 955 (2016), Application(s): Measured peripheral blood levels of serotonin, Abstract; Full Text
Generation of functional human serotonergic neurons from fibroblasts: K.C. Vadodaria, et al.; Mol. Psychiatry 21, 49 (2016), Application(s): Serotonin levels in culture supernatants, Abstract;
Heightened aggressive behavior in mice deficient in aldo-keto reductase 1a (Akr1a): T. Homma, et al.; Behav. Brain Res. 22, 319 (2016), Application(s): Plasma serotonin levels measured in mice, Abstract;
M30 Antagonizes Indoleamine 2,3-Dioxygenase Activation and Neurodegeneration Induced by Corticosterone in the Hippocampus: C.S. Lam, et al.; PLoS One 11, e0166966 (2016), Application(s): Serotinin levels in the hippocampal tissue lysate were measured , Abstract; Full Text
Serotonin Activates Bacterial Quorum Sensing and Enhances the Virulence of Pseudomonas aeruginosa in the Host: L.D. Knecht, et al.; EBioMedicine 9, 161 (2016), Application(s): Serotonin determination in mouse intestinal homogenates and stool samples, Abstract;
Suicide, Impulsivity and its Relationship to Platelet Serotonin Levels: A. Kumar, et al.; IJCMR 3, 3077 (2016), Application(s): Serotonin levels in Human platelets were measured, Full Text
Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE: Z. Ali, et al.; Gene 588, 163 (2016), Application(s): Measured plasma 5-HT levels, Abstract;
Use of the RatLoft decreases pup mortality in lactating mice: S.R. Weaver, et al.; Lab. Anim. 50, 370 (2016), Application(s): Serotonin levels in mouse serum, Abstract;
Altered serotonin (5-HT) 1D and 2A receptor expression may contribute to defective insulin and glucagon secretion in human type 2 diabetes: H. Bennet, et al.; Peptides 71, 113 (2015), Application(s): ELISA using human islets, Abstract;
Elevated levels of 14-3-3 proteins, serotonin, gamma enolase and pyruvate kinase identified in clinical samples from patients diagnosed with colorectal cancer: P. Dowling, et al.; Clin. Chim. Acta 441, 133 (2015), Application(s): EIA using human plasma samples, Abstract;
Human tear serotonin levels correlate with symptoms and signs of dry eye: P. Chhadva, et al.; Ophthalmology 122, 1675 (2015), Application(s): Serotonin levels in human tears, Abstract;
Supplement of 5-hydroxytryptophan before induction suppresses inflammation and collagen-induced arthritis: T.H. Yang, et al.; Arthritis Res. Ther. 17, 364 (2015), Application(s): Serotonin levels in mouse serum, Abstract; Full Text
Leptin, adiponectin and serotonin levels in lean and obese dogs: H.J. Park, et al.; BMC Vet. Res. 10, 113 (2014), Application(s): Serotonin levels in dog plasma, Abstract; Full Text
Peripheral Serotonin Regulates Maternal Calcium Trafficking in Mammary Epithelial Cells during Lactation in Mice: J. Laporta, et al.; PLoS One 9, e110190 (2014), Application(s): EIA using mouse serum, Abstract; Full Text
Serotonin regulates calcium homeostasis in lactation by epigenetic activation of hedgehog signaling: J. Laporta, et al.; Mol. Endocrinol. 28, 1866 (2014), Application(s): EIA using mouse serum, Abstract;
Feeding 5-hydroxy-l-tryptophan during the transition from pregnancy to lactation increases calcium mobilization from bone in rats: J. Laporta, et al.; Domest. Anim. Endocrinol. 44, 176 (2013), Application(s): EIA using rat serum, Abstract;
Lactobacillus johnsonii inhibits indoleamine 2,3-dioxygenase and alters tryptophan metabolite levels in BioBreeding rats: R. Valladares, et al.; FASEB J. 27, 1711 (2013), Application(s): EIA using rat serum, Abstract;
Serotonin (5-HT) affects expression of liver metabolic enzymes and mammary gland glucose transporters during the transition from pregnancy to lactation: J. Laporta, et al.; PLoS One 8, e57847 (2013), Application(s): EIA using rat serum, Abstract; Full Text
Short communication: Circulating serotonin (5-HT) concentrations on day 1 of lactation as a potential predictor of transition-related disorders: J. Laporta, et al.; J. Dairy Sci. 96, 5146 (2013), Application(s): EIA using bovine serum and plasma, Abstract;
Local serotonin mediates cyclic strain-induced phenotype transformation, matrix degradation, and glycosaminoglycan synthesis in cultured sheep mitral valves: C.M. Lacerda, et al.; Am. J. Physiol. Heart Circ. Physiol. 302, H1983 (2012), Application(s): EIA using media of sheep mitral valves, Abstract;
Perinatal growth restriction is not related to higher intestinal distribution and increased serum levels of 5-hydroxytryptamin in piglets: S. Willemen, et al.; J. Anim. Sci. 90S4, 305 (2012), Application(s): EIA using piglet serum, Abstract;
Static and cyclic tensile strain induce myxomatous effector proteins and serotonin in canine mitral valves: C.M. Lacerda, et al.; J. Vet. Cardiol. 14, 223 (2012), Application(s): EIA using media of canine mitral valves, Abstract;
Tryptophan hydroxylase-1 regulates immune tolerance and inflammation: E.C. Nowak, et al.; J. Exp. Med. 209, 2127 (2012), Application(s): EIA using mouse serum, Abstract; Full Text

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