Product Details
| Alternative Name: | X-box binding protein 1, Tax-responsive element-binding protein 5, TREB5, XBP2 |
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| Clone: | 8F6.11 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | Synthetic peptide corresponding to the sequence near the C-terminus of human XBP-1, spliced isoform XBP1(S). |
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| UniProt ID: | P17861 |
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| Source: | Purified from concentrated hybridoma tissue culture supernatant. |
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| Species reactivity: | Human, Mouse
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| Applications: | ELISA, ICC, IP, WB
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| Recommended Dilutions/Conditions: | Immunocytochemistry (1:100)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~60kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of misfolded proteins in the endoplasmic reticulum (ER). XBP-1 mRNA is spliced by IRE1p under ER stress, resulting in a 371 amino acid protein (XBP-1s) which is translocated to the nucleus where it binds to the regulatory elements of downstream genes. XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones grp78 and grp94. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of XBP-1S mAb (8F6.11) (Prod. No. ENZ-ABS255): Lane 1: MW markers, Lane 2: mock-transfected CHO cell, Lane 3: XBP-1S (spliced isoform)-transfected CHO cell. The observed molecular weight of XBP-1S is ~60 kDa (calculated MW: 40 kDa).
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Product Literature References
Nitazoxanide inhibits paramyxovirus replication by targeting the Fusion protein folding: role of glycoprotein-specific thiol oxidoreductase ERp57: S. Piacentini, et al.; Sci. Rep.
8, 10425 (2018),
Abstract;
Full Text
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