Product Details
| Alternative Name: | P4HB, protein disulfide isomerase |
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| Host: | Rabbit |
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| Immunogen: | Purified bovine liver Protein Disulfide Isomerase. |
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| UniProt ID: | P05307 |
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| GenBank ID: | M17596 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Sheep, Xenopus
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| Applications: | ICC, IHC (PS), IP, WB
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| Recommended Dilutions/Conditions: | Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~58kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | The mammalian PDI (Protein disulfide-isomerase) family encompasses several highly divergent proteins which are involved in the processing and maturation of secretory proteins in the endoplasmic reticulum by catalyzing the rearrangement of disulfide bonds. |
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| Regulatory Status: | RUO - Research Use Only |
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Immunohistochemistry analysis of human spleen tissue stained with PDI, pAb at 10µg/ml.
Western Blot Analysis of PDI: Lane 1: MWM, Lane 2: PDI (human), (recombinant) (His-tag) (Prod. No.ADI-SPP-891), Lane 3: HeLa, (cell lysate) (Prod. No.ADI-LYC-HL100), Lane 4: Rat Testis Tissue Lysate, Lane 5: Mouse Spleen Tissue Lysate.
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Product Literature References
Protein Disulfide Isomerase A3 Regulates Influenza Neuraminidase Activity and Influenza Burden in the Lung: N. Chamberlain, et al.; Int. J. Mol. Sci.
23, 1078 (2022),
Abstract;
Protein disulfide isomerase modulation of TRPV1 controls heat hyperalgesia in chronic pain: Y. Zhang, et al.; Cell Rep.
39, 110625 (2022),
Abstract;
DGAT2 stability is increased in response to DGAT1 inhibition in gene edited HepG2 cells: P.J. McFie, et al.; Biochim. Biophys. Acta Mol. Cell Biol. Lipids
1866, 158991 (2021),
Abstract;
Plasma biomarkers for prediction of early tumor recurrence after resection of pancreatic ductal adenocarcinoma: M.C. Rittmann, et al.; Sci. Rep.
11, 7499 (2021),
Abstract;
Full Text
Suppression of ER-stress induction of GRP78 as an anti-neoplastic mechanism of the cardiac glycoside Lanatoside C in pancreatic cancer: Lanatoside C suppresses GRP78 stress inductio: D.P. Ha, et al.; Neoplasia
23, 1213 (2021),
Abstract;
EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD: G. George, et al.; Elife
9, e53455 (2020),
Abstract;
Full Text
Elevated Peripheral Myelin Protein 22, Reduced Mitotic Potential, and Proteasome Impairment in Dermal Fibroblasts from Charcot-Marie-Tooth Disease Type 1A Patients: S. Lee, et al.; Am. J. Pathol.
188, 728 (2018),
Abstract;
Ixeris dentata Extract Increases Salivary Secretion through the Regulation of Endoplasmic Reticulum Stress in a Diabetes-Induced Xerostomia Rat Model: K. Raj Bhattarai, et al.; Int. J. Mol Sci.
19, 1059 (2018),
Abstract;
Full Text
ER-associated degradation is required for vasopressin prohormone processing and systemic water homeostasis: G. Shi, et al.; J. Clin. Invest.
127, 3897 (2017),
Application(s): Western Blot,
Abstract;
Full Text
Estrogen-enhanced apical and basolateral secretion of apolipoprotein B-100 by polarized trophoblast-derived BeWo cells: M. Kamper, et al.; Biochimie
138, 116 (2017),
Abstract;
mTORC1 inhibition in cancer cells protects from glutaminolysis-mediated apoptosis during nutrient limitation: V.H. Villar, et al.; Nat. Commun.
8, 14124 (2017),
Abstract;
Full Text
Jagn1 Is Induced in Response to ER Stress and Regulates Proinsulin Biosynthesis: C. Nosak, et al.; PLoS One
11, e0149177 (2016),
Application(s): Immunofluorescence microscopy,
Abstract;
Full Text
Characterization and Mechanism of Stress-induced Translocation of 78-kilodalton Glucose Regulated Protein (GRP78) to the Cell Surface: Y.L. Tsai, et al.; J. Biol. Chem.
290, 8049 (2015),
Application(s): Western Blotting,
Abstract;
Full Text
Insufficient glucose supply is linked to hypothermia upon cold exposure in mice lacking phosphatidylethanolamine N-methyltransferase: X. Gao, et al.; J. Lipid. Res.
56, 1701 (2015),
Application(s): Western Blot,
Abstract;
Full Text
IRE1α is an endogenous substrate of endoplasmic-reticulum-associated degradation: S. Sun, et al.; Nat. Cell Biol.
17, 1546 (2015),
Application(s): Western Blot,
Abstract;
Full Text
On the Role of Protein Disulfide Isomerase in the Retrograde Cell Transport of Secreted Phospholipases A2: J. Oberčkal, et al.; PLoS One
10, e0120692 (2015),
Application(s): Immunoprecipitation ,
Abstract;
Full Text
Islets of Langerhans from prohormone convertase-2 knockout mice show α-cell hyperplasia and tumorigenesis with elevated α-cell neogenesis: H.B. Jones, et al.; Int. J. Exp. Pathol.
95, 29 (2014),
Abstract;
Impaired ERAD and ER stress are early and specific events in polyglutamine toxicity: S. Lindquist, et al. ; Genes Dev.
22, 3308 (2008),
Application(s): WB using human cell lysates,
Abstract;
Membrane topography of human phosphatidylethanolamine N-methyltransferase: D.E. Vance, et al. ; J. Biol. Chem.
278, 2956 (2003),
Application(s): WB using monkey samples,
Abstract;
Conserved furin cleavage site not essential for secretion and integration of ZP3 into the extracellular egg coat of transgenic mice: J. Dean, et al. ; Mol. Cell. Biol.
22, 3111 (2002),
Application(s): ICC using mouse samples,
Abstract;
Maturation of lipoprotein lipase in the endoplasmic reticulum: concurrent formation of functional dimers and inactive aggregates: M.H. Doolittle, et al. ; J. Biol. Chem.
277, 10727 (2002),
Application(s): IP using human samples,
Abstract;
Partitioning of the matrix fraction of the Golgi apparatus during mitosis in animal cells: G. Warren, et al. ; Science
295, 848 (2002),
Application(s): WB using rat samples,
Abstract;
Trafficking of lysosomal cathepsin B-green fluorescent protein to the surface of thyroid epithelial cells involves the endosomal/lysosomal compartment: K. Brix, et al. ; J. Cell. Sci.
115, 4877 (2002),
Application(s): ICC using rat samples,
Abstract;
Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells: A.H. Futerman, et al. ; J. Biol. Chem.
277, 35642 (2002),
Application(s): ICC using monkey samples,
Abstract;
Protein-disulfide isomerase (PDI) in FRTL5 cells - pH-dependent thyroglobulin/PDI interactions determine a novel PDI function in the post-endoplasmic reticulum of thyrocytes: R. Miquelis, et al. ; J. Biol. Chem.
275, 1920 (2000),
Application(s): IP using rat samples,
Abstract;
Specificity in substrate binding by protein folding catalysts: tyrosine and tryptophan residues are the recognition motifs for the binding of peptides to the pancreas-specific protein disulfide isomerase PDIp: P. Klappa, et al. ; Protein Sci.
9, 758 (2000),
Application(s): WB using sheep samples,
Abstract;
Type I alpha phosphatidylinositol 4-phosphate 5-kinase is a putative target for increased intracellular phosphatidic acid: I. Merida, et al. ; FEBS Lett.
476, 160 (2000),
Application(s): ICC using porcine samples,
Abstract;
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