Replaces Prod. #: ALX-804-031
Product Details
| Alternative Name: | Endoplasmin, Tra1, Hsp90B1, Gp96 |
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| Clone: | 9G10 |
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| Host: | Rat |
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| Isotype: | IgG2a |
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| Immunogen: | Native chicken Grp94. |
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| UniProt ID: | P08110 |
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| GenBank ID: | M14772 |
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| Source: | Purified from ascites. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
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| Applications: | ICC, IF, IHC (PS), IP, WB
Electron microscopy
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| Recommended Dilutions/Conditions: | Immunoprecipitation (1:100)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~98kDa by Western blot. |
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| Purity Detail: | Protein G affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol and 0.09% sodium azide. |
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| Handling: | Avoid freeze/thaw cycles. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Grp94 (Glucose-regulated protein 94) is an abundant resident endoplasmic reticulum (ER) lumenal stress protein, which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. Grp94 expression is upregulated by stress conditions such as glucose starvation and heat shock, which promote protein misfolding or unfolding. In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells. |
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| Regulatory Status: | RUO - Research Use Only |
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Western Blot Analysis of Grp94: Lane 1: MW Marker, Lane 2: Grp94 (canine), (recombinant) (Prod No. ADI-SPP-766), Lane 3: HeLa, (cell lysate) (Prod No. ADI-LYC-HL100), Lane 4: Mouse Liver Lysate, Lane 5: Vero Cell Lysate
Immunohistochemistry analysis of human spleen tissue stained with Grp94, mAb (9G10) at 10µg/ml.
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Product Literature References
Potent NKT cell ligands overcome SARS-CoV-2 immune evasion to mitigate viral pathogenesis in mouse models: H. Lu, et al.; PLoS Pathog.
19, e1011240 (2023),
Abstract;
Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer: K. Ekström, et al.; BMC Cancer
22, 50 (2022),
Abstract;
Extracellular vesicles from triple negative breast cancer promote pro-inflammatory macrophages associated with better clinical outcome: M. Tkach, et al.; PNAS
119, e2107394119 (2022),
Abstract;
Pharmacological activation of ATF6 remodels the proteostasis network to rescue pathogenic GABAA receptors: M. Wang, et al.; Cell Biosci.
12, 48 (2022),
Abstract;
Deletion of mFICD AMPylase alters cytokine secretion and affects visual short-term learning in vivo: N. McCaul, et al.; J. Biol. Chem.
297, 100991 (2021),
Abstract;
The heat shock response, determined by QuantiGene multiplex, is impaired in HD mouse models and not caused by HSF1 reduction: C. Gomez-Paredes, et al.; Sci. Rep.
11, 9117 (2021),
Abstract;
TLR or NOD receptor signaling skews monocyte fate decision via distinct mechanisms driven by mTOR and miR-155: A. Coillard, et al.; PNAS
118, e2109225118 (2021),
Abstract;
Exosomes influence the behavior of human mesenchymal stem cells on titanium surfaces: X. Wang, et al.; Biomaterials
230, 119571 (2020),
Application(s): WB,
Abstract;
Protective Effects of Glucose-Related Protein 78 and 94 on Cisplatin-Mediated Ototoxicity: J. Yi, et al.; Antioxidants (Basel)
9, E686 (2020),
Abstract;
Full Text
LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors: Y.L. Fu, et al.; Biochem. Biophys. Res. Commun.
511, 356 (2019),
Abstract;
Molecular profiling and functional analysis of macrophage-derived tumor extracellular vesicles: C. Cianciaruso, et al.; Cell Rep.
27, 3062 (2019),
Application(s): ELISA using culture supernatants,
Abstract;
Full Text
Long Interleukin-22 Binding Protein Isoform-1 Is an Intracellular Activator of the Unfolded Protein Response: P. Gomez-Fernandez, et al.; Front. Immunol.
9, 2934 (2018),
Abstract;
Full Text
Histone deacetylase activity mediates acquired resistance towards structurally diverse HSP90 inhibitors: R.C. Chai, et al.; Mol. Oncol.
11, 567 (2017),
Abstract;
Full Text
Agonist-Mediated Activation of STING Induces Apoptosis in Malignant B Cells: C.A. Tang, et al.; Cancer Res.
76, 2137 (2016),
Abstract;
Full Text
Grp94 Delivers γ-aminobutyric Acid Type A (GABAA) Receptors to Hrd1-Mediatd Endoplasmic Reticulum-Associated Degradation: X.J. Di, et al.; J. Biol. Chem.
291, 9526 (2016),
Application(s): Western blot,
Abstract;
Full Text
Hermes (Rbpms) is a Critical Component of RNP Complexes that Sequester Germline RNAs during Oogenesis: T. Aguero, et al.; J. Dev. Biol.
4, 2 (2016),
Application(s): Immunofluorescence microscopy,
Abstract;
Full Text
Resveratrol augments ER stress and the cytotoxic effects of glycolytic inhibition in neuroblastoma by downregulating Akt in a mechanism independent of SIRT1: R.M. Graham, et al.; Exp. Mol. Med.
48, e210 (2016),
Application(s): Western blot,
Abstract;
Full Text
Subcellular mechanisms involved in apoptosis induced by aminoglycoside antibiotics: Insights on p53, proteasome and endoplasmic reticulum: S. Denamur, et al.; Toxicol. Appl. Pharmacol.
309, 24 (2016),
Application(s): Western blot, LLC-PK1 (Lilly Laboratories Culture-Pig Kidney Type 1) cells,
Abstract;
A Versatile Cell Death Screening Assay Using Dye-Stained Cells and Multivariate Image Analysis: T.J. Collins, et al. ; Assay Drug Dev. Technol.
13, 547 (2015),
Application(s): Western Blot,
Abstract;
Full Text
GP96 is a GARP chaperone and controls regulatory T cell functions: Y. Zhang, et al.; J. Clin. Invest.
125, 859 (2015),
Application(s): Ion Chromatography,
Abstract;
Full Text
Tunicamycin-induced unfolded protein response in the developing mouse brain: H. Wang, et al.; Toxicol. Appl. Pharmacol.
283, 157 (2015),
Application(s): Western Blotting,
Abstract;
Heat shock protein gp96 is a master chaperone for toll-like receptors and is important in the innate function of macrophages: Z. Li, et al. ; Immunity
26, 215 (2007),
Application(s): WB, IP, IF, Flow using mouse & human cell lysates & tissue culture,
Abstract;
Identifying the membrane proteome of HIV-1 latently infected cells: F. Kashanchi, et al. ; J. Biol. Chem.
282, 8207 (2007),
Application(s): WB using human cell lysates,
Abstract;
GRP94 (94 kDa glucose-regulated protein) suppresses ischemic neuronal cell death against ischemia/reperfusion injury: M. Tohyama, et al. ; Eur. J. Neurosci.
18, 829 (2003),
Application(s): ICC using human samples,
Abstract;
Pre-M phase-promoting factor associates with annulate lamellae in Xenopus oocytes and egg extracts: E. Houliston, et al. ; Mol. Biol. Cell
14, 1125 (2003),
Application(s): ICC, WB using xenopus samples,
Abstract;
A novel von Willebrand disease-causing mutation (Arg273Trp) in the von Willebrand factor propeptide that results in defective multimerization and secretion: S. Allen, et al.; Blood
96, 560 (2000),
Application(s): WB using monkey samples,
Abstract;
Expression of stress protein gp96, a tumor rejection antigen, in human colorectal cancer: P.R. Galle, et al. ; Int. J. Cancer
86, 489 (2000),
Application(s): IHC, WB using human samples,
Abstract;
Increase in vulnerability of middle-aged rat brain to lead by cerebral energy depletion: S. Hoyer, et al. ; Brain Res. Bull.
52, 371 (2000),
Application(s): IHC, WB using rat samples,
Abstract;
Participation of GRP94-related protein in secretion of pancreatic bile salt-dependent lipase and in its internalization by the intestinal epithelium: M. Bendayan, et al. ; J. Cell. Sci.
111, 2665 (1998),
Application(s): EM, IP, WB, IHC using rat samples,
Abstract;
Monoclonal antibodies raised against infectious haematopoietic necrosis virus (IHNV) G protein and a cellular 90 kDa protein neutralize IHNV infection in vitro: J.Y. Lee, et al.; J. Gen. Virol.
77, 1731 (1996),
Application(s): Western blot analysis compared to 90 kDa protein,
Abstract;
Full Text
Amino acid deprivation-induced stress response in the bovine renal epithelial cell line NBL-1: induction of HSP 70 by phenylalanine: J.D. McGivan, et al. ; Biochim. Biophys. Acta
1224, 189 (1994),
Application(s): WB using bovine samples,
Abstract;
Structural analysis of chicken oviduct progesterone receptor using monoclonal antibodies to the subunit B protein: W.L. McGuire, et al. ; Biochemistry
23, 4427 (1984),
Application(s): WB using chicken samples,
Abstract;
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