Product Details
| Alternative Name: | CYPR |
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| Host: | Rabbit |
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| Immunogen: | Native rat Cytochrome p450 Reductase. |
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| UniProt ID: | P00388 |
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| Source: | Purified from rabbit serum. |
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| Species reactivity: | Human, Mouse, Rat
Bovine, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
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| Applications: | ICC, IP, WB
Inhibition assay
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| Recommended Dilutions/Conditions: | Western Blot (1:2,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Application Notes: | Detects a band of ~78kDa by Western blot. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Cytochrome p450 is a highly conserved and diverse family of heme-containing proteins that function in the metabolism of numerous endogenous and exogenous substrates.. The human genome encodes 57 p450 genes divided among 18 families, which together account for the diversity of substrates metabolized. p450 is the primary conjugating enzyme in phase I metabolism and detoxification of endogenous and exogenous compounds, and are primarily localized to the inner membranes of mitochondria and the ER. Eukaryotic microsomal cytochrome p450 enzymes receive electrons from the FAD- and FMN-containing enzyme NADPH:cytochrome p450 reductase (CPR). |
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| Regulatory Status: | RUO - Research Use Only |
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Lane 1: MWM, Lane 2: OSP-300, Lane 3: Human Liver Microsomes, Lane 4: Rat Liver Microsomes, Lane 5: Mouse Liver Microsomes, Lane 6: MWM
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Product Literature References
Ginkgo Biloba Extract Neuroprotective Action Is Dependent on HemeOxygenase 1 in Ischemic Reperfusion Brain Injury: S. Dore, et al. ; Stroke
39, 3389 (2008),
Application(s): WB using mouse tissue culture,
Abstract;
Paraquat increases cyanide-insensitive respiration in murine lung epithelial cells by activating an NAD(P)H:paraquat oxidoreductase -Identification of the enzyme as thioredoxin reductase: J. Laskin, et al. ; J. Biol. Chem.
282, 7939 (2007),
Application(s): IA using mouse cell lysates,
Abstract;
Heme oxygenase-1 and NADPH cytochrome P450 reductase expression in experimental allergic encephalomyelitis: an expanded view of the stress response: S.M. LeVine, et al. ; J. Neurochem.
75, 2555 (2000),
Application(s): IP, WB using mouse samples,
Abstract;
Identification of a new endoplasmic reticulum-resident protein recognized by HLA-A24-restricted tumor-infiltrating lymphocytes of lung cancer: A. Yamada, et al. ; Cancer Res.
60, 3550 (2000),
Application(s): WB using monkey samples,
Abstract;
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