Product Details
| Alternative Name: | TXN |
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| Clone: | 2C9 |
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| Host: | Mouse |
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| Isotype: | IgG1 |
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| Immunogen: | E. coli thioredoxin (trxA). |
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| Species reactivity: | E. coli
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| Specificity: | Recognizes trx and trx-tag-f thioredoxin. |
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| Applications: | WB
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| Recommended Dilutions/Conditions: | Western Blot (1µg/ml, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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| Purity Detail: | Protein A affinity purified. |
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| Formulation: | Liquid. In PBS containing 50% glycerol. |
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| Shipping: | Blue Ice |
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| Long Term Storage: | -20°C |
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| Scientific Background: | Thioredoxins act as antioxidants by reducing the proteins by cysteine thiol-disulfide exchange. Several plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in E. coli as fusions with C-terminal Thioredoxin (TrxA) to offer soluble expression of normally insoluble or difficult to express proteins such as cytokines and growth factors. |
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| Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of Thioredoxin fusion protein expressed cell lysate after no induction (1), or induction for 30 min (2), 60 min (3), 90 min (4), 120 min (5), 150 min (6), 180 min (7), 210 min (8)
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Product Literature References
Role of Hsp70 and the Associated Signaling Molecules in Fish Adipocytes during Pollutants Induced Hypoxia: P. Ekambaram, et al.; IOSR J. Environ. Sci. Toxicol. Food Technol.
9, 16 (2015),
Application(s): Western Blot,
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