Product Details
Clone: | PL14 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | 6myc-tag GST fusion protein. |
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UniProt ID: | P01106 (human) |
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Specificity: | Recognizes the Myc-tag of Myc-tagged recombinant proteins. |
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Applications: | ICC, IP, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (2µg/ml) Immunoprecipitation (5µg/ml) Western Blot (1µg/ml, ECL) Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Purity Detail: | Protein A affinity purified. |
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Formulation: | Liquid. In PBS, pH 7.2, containing 50% glycerol. No preservative has been added. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Epitope tagging is a widely accepted technique that fuses an epitope peptide to a certain protein as a marker for gene expression. With this technique, gene expression can be easily monitored on Western blotting, immunoprecipitation and immunofluorescence utilizing an antibody that recognizes such an epitope. The Myc-Tag (EQKLISEEDL) corresponds to the partial peptide sequence of the human c-myc gene product. |
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Regulatory Status: | RUO - Research Use Only |
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Western blot analysis of 293T cells transfected with 6Myc/Heparanase (lane 1) and 6Myc/cdc25 (lane 2), probed with Myc-tag mAb (PL14).
Immunocytochemistry analysis of monkey Cos-7 cells transfected with 6myc-hCAP1, stained with Myc-tag mAb (PL14).
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Product Literature References
ZnT7, a novel mammalian zinc transporter, accumulates zinc in the Golgi apparatus: L. Huang, et al. ; J. Biol. Chem.
278, 4096 (2003),
Application(s): ICC using human samples,
Abstract;
Functional characterization of a novel mammalian zinc transporter, ZnT6: J. Gitschier, et al. ; J. Biol. Chem.
277, 26389 (2002),
Application(s): WB using human samples,
Abstract;
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