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AMH (human), (recombinant)

 
ENZ-PRT195-0010 10 µg 300.00 USD
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Product Specification

Alternative Name:anti-Müllerian hormone, Mullerian-inhibiting substance
 
MW:Expected is 61.2 kDa (main band is 68 kDa with minor bands at 60 kDa and 38 kDa)
 
Source:Produced in HEK 293 cells. AMH (aa 19-560) is fused at the N-terminus to a FLAG®-tag and His-tag.
 
UniProt ID:P03971
 
Concentration:0.1mg/ml when reconstituted as suggested.
 
Formulation:Lyophilized in 1X PBS, pH 7.4.
 
Purity:≥90% (SDS-PAGE)
 
Purity Detail:Purified by multi-step chromatography.
 
Endotoxin Content:≤100EU/mg
 
Biological Activity:Inhibits proliferation of Caski cells (ED50 range is between 0.05-1.0 µg/mL)
 
Reconstitution:Reconstitute with 100μl sterile distilled water.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least 6 months after receipt when stored at -20°C.
 
Handling:After reconstitution, the protein is stable for 1 week. For longer term storage prepare aliquots and store at -80°C. Avoid freeze/thaw cycles.
 
Scientific Background:AMH (anti-Müllerian hormone) is a member of the TGF-β family of genes and has been shown to be present in both males and females. It is known to be expressed in Sertoli cells and is involved in male sexual differentiation. The hormone is present in the ovary of females and has been shown to be a marker of female fertility. AMH was found to inhibit primordial follicle assembly and decrease the initial primordial follicle pool size. Women with polycystic ovarian syndrome (PCOS) exhibit elevated levels of Anti-Müllerian hormone (AMH) which has been found to desensitize ovarian follicles. It has also been shown to inhibit growth of cervical cancer cells via the MIS type II receptor.
 
Technical Info/Product Notes:FLAG is a registered trademark of Sigma-Aldrich Co.
 
ENZ-PRT195 SDS-PAGE
Coomassie stained SDS-PAGE. Lane 1: Molecular weight marker, Lane 2: 1.0 µg, Lane 3: 2.0 µg, Lane 4: 5.0 µg AMH (Prod. No. ENZ-PRT195).
ENZ-PRT195 His-Blot
Western blot analysis of AMH probed with mouse anti-HIS antibody. Lane 1: MWM, Lane 2: 100 ng AMH (Prod. No. ENZ-PRT195) probed HIS antibody.
ENZ-PRT195 Flag-Blot
Western blot analysis of AMH probed with rabbit anti-FLAG antibody. Lane 1: MWM, Lane 2: 100 ng AMH (Prod. No. ENZ-PRT195) probed FLAG antibody.
ENZ-PRT195 Activity Assay
Caski cell growth inhibition assay after treatment with AMH (ENZ-PRT195): Caski cells were placed in stationary media at 5000 cells/well in 96 well plates. They were treated with a titered concentration of AMH diluted in cell culture media. The cells were grown at 37°C / 5% CO2 for 48 hours. Inhibition of growth was measured using a modified MTT cell viability assay. The ED50 range is between 0.05 – 1.0 μg/mL.
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ENZ-PRT195 SDS-PAGE ENZ-PRT195 His-Blot ENZ-PRT195 Flag-Blot ENZ-PRT195 Activity Assay

General Literature References

The influence of circulating anti-Müllerian hormone on ovarian responsiveness to ovulation induction with gonadotrophins in women with polycystic ovarian syndrome: a pilot study : S.A. Amer, et al.; Reprod. Biol. Endocrinol. 11, 115 (2013), Abstract; Full Text
Up-Regulation of SOX9 in Sertoli Cells from Testiculopathic Patients Accounts for Increasing Anti-Mullerian Hormone Expression via Impaired Androgen Receptor Signaling: K.C. Lan, et al.; PLoS ONE 8, e76303 (2013), Abstract; Full Text
Diagnosis of polycystic ovary syndrome (PCOS): revisiting the threshold values of follicle count on ultrasound and of the serum AMH level for the definition of polycystic ovaries : D. Dewailly, et al.; Hum. Reprod. 26, 3123 (2011), Abstract; Full Text
Inhibitory actions of Anti-Müllerian Hormone (AMH) on ovarian primordial follicle assembly: E. E. Nilsson, et al.; PLoS One 6, e20087 (2011), Abstract; Full Text
Mullerian Inhibiting Substance inhibits cervical cancer cell growth via a pathway involving p130 and p107: T.U. Barbie, et al.; PNAS 100, 26 (2003), Abstract; Full Text

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