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Antibody Blocker/Diluent

Ready-to-use antibody blocking buffer and diluent, for use with POLYVIEW® PLUS detection reagents
 
ENZ-ACC108-0100 100 ml Inquire for pricing
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The Antibody Blocker/Diluent can be used to block nonspecific staining due to antibodies during immunostaining. It can also be used as a diluent for the POLYVIEW® PLUS detection reagents or as a primary antibody diluent.

This product is intended for immunohistochemistry and in situ hybridization with formalin-fixed parafiin-embedded tissue sections and frozen sections. The blocker/diluent may also be used with blood smears, cytosmears, and cell preparations.

Product Specification

Applications:IHC
ISH (in situ hybridization)
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Technical Info/Product Notes:The Antibody Blocker/Diluent is a unique blocking solution used to reduce nonspecific background staining. It is a universal blocking agent which eliminates the need to match blocker to antibody species. In addition to immunohistochemistry, the blocker/diluent can also be used for in situ hybridization.

The Antibody Blocker/Diluent can also be used as a diluent for POLYVIEW® PLUS HRP (anti-rabbit) reagent (ENZ-ACC103), POLYVIEW® PLUS HRP (anti-mouse) reagent (ENZ-ACC104), and POLYVIEW® PLUS AP (anti-rabbit) reagent (ENZ-ACC110).  It can also serve as a diluent for the primary antibody.
 
Protocol:
Recommended protocol for IHC (immunohistochemistry)
  1. Arrange slides of formalin fixed paraffin-embedded (FFPE) tissue sections in a slide holder.
  2. Heat in drying oven at 55-60°C for 20 minutes.
  3. Deparaffinize with xylene or xylene substitute for 10 minutes.
  4. Follow with 100% ethanol for 6 minutes.
  5. Rehydrate slides for one minute in each step - 90%, 70%, 50% ethanol and in distilled or deionized water.
  6. Incubate the tissue with peroxidase block for 5 minutes.
  7. Wash with PBST for 2 minutes.
  8. Retrieve antigen with  Antigen Retrieval Reagent (Citrate, pH 6) or Antigen Retrieval Reagent (Tris-EDTA, pH 9) for 20 minutes at 99°C.
  9. Wash for 5 minutes with PBST.
  10. Wipe the excess liquid around the section on the glass slide and encircle the tissue section with a PAP pen.
  11. Dry slide for 10 seconds.
  12. Incubate sections with Antibody Buffer/Diluent for 10 minutes to block non-specific antibody binding.
  13. Researcher needs to optimize the concentrations and incubation times for primary antibodies (recommended 20 - 30 minutes)
  14. Wash for 5 min with PBST.
  15. After adding primary antibody, use POLYVIEW® PLUS Detection reagents and HIGHDEF® Chromogens to visualize antigens.
 

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