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SEEBRIGHT® Green 496 dUTP

 
ENZ-42831 25 nmol 263.00 USD
Do you need bulk/larger quantities?
 
SEEBRIGHT® Green 496 [5-Fluorescein] dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, random prime labeling, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. SEEBRIGHT® Green 496 dUTP can also be used for multicolor fluorescence labeling.

This labeled dUTP can be used with the Nick Translation DNA Labeling System 2.0 (Prod. No. ENZ-GEN111).

Product Specification

Alternative Name:5-Fluorescein dUTP
 
Quantity:Sufficient for approximately 49 reactions, following the recommended protocol of Prod. No. ENZ-GEN111.
 
Concentration:1mM
 
Formulation:Liquid. Solution in water.
 
Excitation maximum:496 nm
 
Emission maximum:520 nm
 
Extinction Coefficient:85,000 M-1 cm-1 (496 nm in TE [10 mM TRIS, pH 8.0, 1 mM EDTA])
 
Correction Factor (260 nm):0.32
 
Correction Factor (280 nm):0.20
 
Purity:≥93% (HPLC)
 
Purity Detail:Purified by ion-exchange chromatography.
 
Appearance:Yellow-green liquid and orange frozen solution.
 
Applications:FISH
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-20°C
 
Use/Stability:Stable for at least one year after receipt when stored as recommended.
 
Handling:Protect from light. Avoid freeze/thaw cycles.
 
Technical Info/Product Notes:Several of Enzo’s products and product applications are covered by US and foreign patents and patents pending.

Application Notes:
Assessing the Effects of Humidity on FISH Using the Nick Translation DNA Labeling System 2.0
 
 
SEEBRIGHT™ Green 496 dUTP Table of Fluorescence emission profiles
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
SEEBRIGHT™ Green 496 dUTP Nick translation
Blood Sample hybridized with FISH probes made with Nick translation DNA labeling system (Prod No. ENZ-42910) and Orange 552 dUTP (Prod No. ENZ-42842) or Green 496 dUTP (Prod No. ENZ-42831). [telomere 7p (spectrum orange, Orange 552 dUTP) RP11-452K21 en 7q (spectrum green, Green 496 dUTP)] Analysis with an epifluorescence microscope using DAPI, FITC and TRITC single band filters. Courtesy of GH Necker-Enfants Malades (Paris, FR).
SEEBRIGHT™ Green 496 dUTP Fluorescence emission profiles - Graph
Fluorescence emission profiles of available fluorescent labeled dUTPs. The dye-dUTPs are designed to perform especially well in multi-color applications, such as in situ hybridization and microarray analysis.
SEEBRIGHT™ Green 496 dUTP (lyophilized) image
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SEEBRIGHT™ Green 496 dUTP Table of Fluorescence emission profiles SEEBRIGHT™ Green 496 dUTP Nick translation SEEBRIGHT™ Green 496 dUTP Fluorescence emission profiles - Graph SEEBRIGHT™ Green 496 dUTP (lyophilized) image

Product Literature References

Live imaging of marked chromosome regions reveals their dynamic resolution and compaction in mitosis: J.K. Eykelenboom, et al.; J. Cell Biol. 218, 1531 (2019), Application(s): Generation of FISH probes, Abstract; Full Text
Loss of p53 causes stochastic aberrant X-chromosome inactivation and female-specific neural tube defects: A.R.D. Delbridge, et al.; Cell Rep. 27, 442 (2019), Application(s): Generation of FISH probes, Abstract;
Structural and spatial chromatin features at developmental gene loci in human pluripotent stem cells: H. Ikeda, et al.; Nat. Commun. 8, 1616 (2017), Application(s): Generation of FISH probes, Abstract; Full Text
ATAC-see reveals the accessible genome by transposase-mediated imaging and sequencing: X. Chen, et al.; Nat. Methods 13, 1013 (2016), Application(s): Generation of FISH probes, Abstract; Full Text
Subtyping of renal cortical neoplasms in fine needle aspiration biopsies using a decision tree based on genomic alterations detected by fluorescence in situ hybridization: B. Gowrishankar, et al.; BJU Int. 114, 881 (2014), Abstract; Full Text
Development and characterization of wheat-Ae. searsii Robertsonian translocations and a recombinant chromosome conferring resistance to stem rust: W. Liu, et al.; Theor. Appl. Genet. 122, 1537 (2011), Abstract;
Frequency of aneuploidy related to age in porcine oocytes: M. Hornak, et al.; PLoS One 7, e18893 (2011), Abstract; Full Text

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