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Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2)

Gold standard antibody for detection of ubiquitinylated proteins.
BML-PW8810-0100 100 µg 154.00 USD
BML-PW8810-0500 500 µg 615.00 USD
Do you need bulk/larger quantities?
  • Detects K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins
  • Most cited multi-ubiquitin antibody with over 500 citations
  • Validated for WB, IP, IHC, and ELISA applications
  • Available as HRP, biotin, ATTO 488, and fluorescein labeled conjugates
The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated and monoubiquitinylated proteins regardless of linkage location but not free ubiquitin.

Product Specification

Immunogen:Poly-ubiquitinylated lysozyme.
UniProt ID:P0CG47 (UBB), P0CG48 (UBC)
Species reactivity:Species independent
Specificity:Recognizes mono- and polyubiquitinylated conjugates.
Crossreactivity:Does not cross-react with free ubiquitin.
Applications:ELISA, IHC, IP, WB
Recommended Dilutions/Conditions:Immunoprecipitation (care must be taken as MAb to Mono- and polyubiquitinylated conjugates (FK2) demonstrates affinity for both free ubiquitin and multi-ubiquitinylated species when immobilized)
Western Blot (1:100-1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Purified.
Formulation:Liquid. In PBS containing 0.1% sodium azide.
Handling:Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month. Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) Western blot
Figure 1: Western blot of multi-ubiquitin chains using MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) (lanes A-C) and MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) (lanes D-F).

Lanes A & D: K48-linked chains.Lanes B & E: K29-linked chains. Lanes C & F: K63-linked chains.

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Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2) Western blot

Product Literature References

Histone deacetylase inhibitor SAHA treatment prevents the development of heart failure after myocardial infarction via an induction of heat-shock proteins in rats: S. Nagata, et al.; Biol. Pharm. Bull. (2019), Abstract;
Measuring Antibacterial Autophagy: K.B. Boyle, et al.; Methods Mol. Biol. 1880, 679 (2019), Abstract;
Alteration of Bcl11b upon stimulation of both MAP Kinase- and Gsk3-dependent signaling pathways in double negative thymocytes: W.H. Selman, et al.; Biochem. Cell Biol. (2018), Abstract;
BLM Potentiates c-Jun Degradation and Alters Its Function as an Oncogenic Transcription Factor: R. Priyadarshini, et al.; Cell Rep. 24, 947 (2018), Abstract;
Distinct subcellular changes in proteasome activity and linkage-specific protein polyubiquitination in the amygdala during the consolidation and reconsolidation of a fear memory: S.A. Orsi, et al.; Neurobiol. Learn. Mem. 157, 1 (2018), Abstract;
ESCRT Proteins Control the Dendritic Morphology of Developing and Mature Hippocampal Neurons: M. Firkowska, et al.; Mol. Neurobiol. (2018), Abstract; Full Text
Essential function of VCP/p97 in infection cycle of the nucleopolyhedrovirus AcMNPV in Spodoptera frugiperda Sf9 cells: Y.V. Lyupina, et al.; Virus Res. 253, 68 (2018), Abstract;
FKBP8 protects the heart from hemodynamic stress by preventing the accumulation of misfolded proteins and endoplasmic reticulum-associated apoptosis in mice: T. Misaka, et al.; J. Mol. Cell. Cardiol. 114, 93 (2018), Abstract; Full Text
Genome-Wide Bimolecular Fluorescence Complementation-Based Proteomic Analysis of Toxoplasma gondii ROP18's Human Interactome Shows Its Key Role in Regulation of Cell Immunity and Apoptosis: J. Xia, et al.; Front. Immunol. 9, 61 (2018), Abstract; Full Text
Inactivation of USP14 Perturbs Ubiquitin Homeostasis and Delays the Cell Cycle in Mouse Embryonic Fibroblasts and in Fruit Fly Drosophila: J.H. Lee, et al.; Cell. Physiol. Biochem. 47, 67 (2018), Abstract;
Mfn2 ubiquitination by PINK1/parkin gates the p97-dependent release of ER from mitochondria to drive mitophagy: G.L. McLelland, et al.; Elife 7, e32866 (2018), Abstract; Full Text
Pathogenic mutation in the ALS/FTD gene, CCNF, causes elevated Lys48-linked ubiquitylation and defective autophagy: A. Lee, et al.; Cell. Mol. Life Sci. 75, 335 (2018), Abstract;
PB1 and UBA domains of p62 are essential for aggresome-like induced structure formation: M. Cabe, et al.; Biochem. Biophys. Res. Commun. 503, 2306 (2018), Abstract;
Quantitative Analysis of the Brain Ubiquitylome in Alzheimer's Disease: M.H. Abreha, et al.; Proteomics 18, e1800108 (2018), Abstract; Full Text
RBX1-mediated ubiquitination of SESN2 promotes cell death upon prolonged mitochondrial damage in SH-SY5Y neuroblastoma cells: A. Kumar & C. Shaha; Mol. Cell. Biochem. 446, 1 (2018), Abstract;
Stimulation of exosome release by extracellular DNA is conserved across multiple cell types: D. Iliev, et al.; FEBS J. (2018), Abstract;
The effect of acute oral phosphatidic acid ingestion on myofibrillar protein synthesis and intracellular signaling in older males: B. Smeuninx, et al.; Clin. Nutr. (2018), Abstract;
Vps13D Encodes a Ubiquitin-Binding Protein that Is Required for the Regulation of Mitochondrial Size and Clearance: A.L. Anding, et al.; Curr. Biol. 28, 287 (2018), Abstract;
ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability: P. Haahr, et al.; Mol. Cell 70, 165 (2018), Abstract;
A High-Throughput Screening Strategy for Development of RNF8-Ubc13 Protein-Protein Interaction Inhibitors: E. Weber, et al.; SLAS Discov. 22, 316 (2017), Abstract;
A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome Spaciousness and Integrity: L. Schnettger, et al.; Cell Host Microbe 21, 619 (2017), Abstract; Full Text
Alternative exon skipping biases substrate preference of the deubiquitylase USP15 for mysterin/RNF213, the moyamoya disease susceptibility factor: Y. Kotani, et al.; Sci. Rep. 7, 44293 (2017), Abstract; Full Text
Autophagosomal Content Profiling Reveals an LC3C-Dependent Piecemeal Mitophagy Pathway: F. Le Guerroué, et al.; Mol. Cell 68, 786 (2017), Abstract;
Azithromycin attenuates myofibroblast differentiation and lung fibrosis development through proteasomal degradation of NOX4: K. Tsubouchi, et al.; Autophagy 13, 1420 (2017), Abstract; Full Text
Cryptic amyloidogenic elements in mutant NEFH causing Charcot-Marie-Tooth 2 trigger aggresome formation and neuronal death: A. Jacquier, et al.; Acta Neuropathol. Commun. 5, 55 (2017), Abstract; Full Text
Drosophila Vps13 Is Required for Protein Homeostasis in the Brain: J.J. Vonk, et al.; PLoS One 12, e0170106 (2017), Abstract; Full Text
Early involvement of lysosome dysfunction in the degeneration of cerebral cortical neurons caused by the lipid peroxidation product 4-hydroxynonenal: S. Zhang, et al.; J. Neurochem. 140, 941 (2017), Application(s): WB and IF on cortical neurons, Abstract; Full Text
Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane: J. Schmuckli-Maurer, et al.; PLoS One 12, e0183797 (2017), Application(s): Indirect immunofluorescence, Abstract; Full Text
Kenny mediates selective autophagic degradation of the IKK complex to control innate immune responses: R. Tusco, et al.; Nat. Commun. 8, 1264 (2017), Abstract; Full Text
Legionella RavZ Plays a Role in Preventing Ubiquitin Recruitment to Bacteria-Containing Vacuoles: T. Kubori, et al.; Front. Cell. Infect. Microbiol. 7, 384 (2017), Abstract; Full Text
Lys48 ubiquitination during the intraerythrocytic cycle of the rodent malaria parasite, Plasmodium chabaudi: L. Gonzalez-Lopez, et al.; PLoS One 12, e0176533 (2017), Abstract; Full Text
Methods to Investigate EGFR Ubiquitination: A. Conte, et al.; Methods Mol. Biol. 1652, 81 (2017), Abstract;
Multimodal actions of the phytochemical sulforaphane suppress both AR and AR-V7 in 22Rv1 cells: Advocating a potent pharmaceutical combination against castration-resistant prostate cancer: N. Khurana, et al.; Oncol. Rep. 38, 2774 (2017), Abstract; Full Text
Replication-Coupled Dilution of H4K20me2 Guides 53BP1 to Pre-replicative Chromatin: S. Pellegrino, et al.; Cell Rep. 19, 1819 (2017), Abstract; Full Text
The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites: K. Ha, et al.; Nat. Commun. 8, 15751 (2017), Abstract; Full Text
Two Antagonistic MALT1 Auto-Cleavage Mechanisms Reveal a Role for TRAF6 to Unleash MALT1 Activation: S. Ginster, et al.; PLoS One 12, e0169026 (2017), Abstract; Full Text
Ubiquitin-specific protease 21 stabilizes BRCA2 to control DNA repair and tumor growth: J. Liu, et al.; Nat. Commun. 8, 137 (2017), Abstract; Full Text
Understanding renal nuclear protein accumulation: an in vitro approach to explain an in vivo phenomenon: L. Luks, et al.; Arch. Toxicol. 91, 3599 (2017), Abstract;
Yeast phospholipase C is required for stability of casein kinase I Yck2p and expression of hexose transporters: T. Zhang, et al.; FEMS Microbiol. Lett. 364, fnx227 (2017), Abstract; Full Text
Caveolin-1 is an aggresome-inducing protein: A. Tiwari, et al.; Sci. Rep. 6, 38681 (2016), Abstract; Full Text
Comparison of the effects of a truncating and a missense MYBPC3 mutation on contractile parameters of engineered heart tissue: P.J. Wijnker, et al.; J. Mol. Cell. Cardiol. 97, 82 (2016), Abstract;
Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-κB: H. Soh, et al.; Mol. Cell. Biol. 36, 1776 (2016), Abstract; Full Text
Ehrlichia secretes Etf-1 to induce autophagy and capture nutrients for its growth through RAB5 and class III phosphatidylinositol 3-kinase: M. Lin, et al.; Autophagy 12, 2145 (2016), Abstract; Full Text
Formaldehyde Is a Potent Proteotoxic Stressor Causing Rapid Heat Shock Transcription Factor 1 Activation and Lys48-Linked Polyubiquitination of Proteins: S. Ortega-Atienza, et al.; Am. J. Pathol. 186, 2857 (2016), Abstract; Full Text
Intracellular glutathione determines bortezomib cytotoxicity in multiple myeloma cells: K.K. Starheim, et al.; Blood Cancer J. 6, e446 (2016), Application(s): Reagent, gene expression analysis, Abstract; Full Text
Intracellular Salmonella induces aggrephagy of host endomembranes in persistent infections: N. Lopez-Montero, et al.; Autophagy 12, 1886 (2016), Abstract; Full Text
Involvement of PARK2-Mediated Mitophagy in Idiopathic Pulmonary Fibrosis Pathogenesis: K. Kobayashi, et al.; J. Immunol. 197, 504 (2016), Abstract;
Loss of NEDD4 contributes to RTP801 elevation and neuron toxicity: implications for Parkinson's disease: M. Canal, et al.; Oncotarget 7, 58813 (2016), Abstract; Full Text
Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability: S.S. Kharat, et al.; Oncogene 35, 125 (2016), Application(s): Immunostaining, Nu-PAGE gel, Abstract;
Npas4 transcription factor expression is regulated by calcium signalling pathways and prevents tacrolimus-induced cytotoxicity in pancreatic beta cells: T. Speckmann, et al.; J. Biol. Chem. 291, 2682 (2016), Application(s): Western blot, Abstract; Full Text
Regulation of Monocarboxylic Acid Transporter 1 Trafficking by the Canonical Wnt/β-Catenin Pathway in Rat Brain Endothelial Cells Requires Cross-talk with Notch Signaling: Z. Lui, et al.; J. Biol. Chem. 291, 8059 (2016), Abstract; Full Text
Regulation of Transforming Growth Factor β-Activated Kinase Activation by Epigallocatechin-3-Gallate in Rheumatoid Arthritis Synovial Fibroblasts: Suppression of K(63) -Linked Autoubiquitination of Tumor Necrosis Factor Receptor-Associated Factor 6: A.K. Singh, et al.; Arthritis Rheumatol. 68, 347 (2016), Abstract; Full Text
The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila: L. Koerver, et al.; Cell Death Differ. 23, 2019 (2016), Application(s): Primary antibody for western blot and for fluorescent staining, Abstract; Full Text
The FANCD2-FANCI complex is recruited to DNA interstrand crosslinks before monoubiquitination of FANCD2: C.C. Liang, et al.; Nat. Commun. 7, 12124 (2016), Abstract; Full Text
The Molecular Chaperone Hsp70 Promotes the Proteolytic Removal of Oxidatively Damaged Proteins by the Proteasome: S. Reeg, et al.; Free Radic. Biol. Med. 99, 153 (2016), Application(s): Immunoblot Analysis, Abstract;
Ubiquitin Ligase NEDD4 Regulates PPARγ Stability and Adipocyte Differentiation in 3T3-L1 Cells: J.J. Li, et al.; Sci. Rep. 6, 38550 (2016), Abstract; Full Text
Ubiquitination of Lysine 867 of the Human SETDB1 Protein Upregulates Its Histone H3 Lysine 9 (H3K9) Methyltransferase Activity: K. Ishimoto, et al.; PLoS One 11, e0165766 (2016), Application(s): Immunoblotting, Abstract; Full Text
USP7 is essential for maintaining Rad18 stability and DNA damage tolerance: A. Zlatanou, et al.; Oncogene 35, 965 (2016), Application(s): Western Blot, Abstract;
αB-Crystallin Interacts with Nav1.5 and Regulates Ubiquitination and Internalization of Cell Surface Nav1.5: Y. Huang, et al.; J. Biol. Chem. 291, 11030 (2016), Abstract; Full Text
A mechanism for the suppression of homologous recombination in G1 cells: A. Orthwein, et al.; Nature 528, 422 (2015), Abstract; Full Text
A phosphoinositide-binding cluster in cavin1 acts as a molecular sensor for cavin1 degradation: V.A. Tillu, et al.; Mol. Biol. Cell 26, 3561 (2015), Abstract; Full Text
A point mutation in the ubiquitin ligase RNF170 that causes autosomal dominant sensory ataxia destabilizes the protein and impairs inositol 1,4,5-trisphosphate receptor-mediated Ca2+ signaling: F.A. Wright, et al.; J. Biol. Chem. 290, 13948 (2015), Abstract; Full Text
Aneuploidy causes premature differentiation of neural and intestinal stem cells: D. Gogendeau, et al.; Nat. Commun. 6, 8894 (2015), Application(s): Immunohistochemistry, Abstract;
BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy: S. Kobayashi, et al.; PNAS 112, 7027 (2015), Abstract; Full Text
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;
Differential use of autophagy by primary dendritic cells specialized in cross-presentation: J.D. Mintern, et al.; Autophagy 11, 906 (2015), Abstract; Full Text
Effects of resveratrol on growth and skeletal muscle physiology of juvenile southern flounder: W.N. Wilson, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 183, 27 (2015), Application(s): Dot blot using fish (flounder) muscle samples, Abstract;
Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage: T. Thorslund, et al.; Nature 10, 1038 (2015), Application(s): Immunofluorescence staining and microscopy, Abstract;
Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages: J.A. Awuh, et al.; PNAS 112, E4272 (2015), Abstract; Full Text
Negative Regulation of CARD11 Signaling and Lymphoma Cell Survival by the E3 Ubiquitin Ligase RNF184: S.M. Pedersen, et al.; Mol. Cell. Biol. 36, 794 (2015), Abstract; Full Text
p62/Sequestosome-1, Autophagy-related Gene 8, and Autophagy in Drosophila Are Regulated by Nuclear Factor Erythroid 2-related Factor 2 (NRF2), Independent of Transcription Factor TFEB: A. Jain, et al.; J. Biol. Chem. 290, 14945 (2015), Abstract; Full Text
Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes: K.K. Raval, et al.; J. Biol. Chem. 290, 3121 (2015), Application(s): Western blot using human cardiomyocyte lysates, Abstract; Full Text
Short-term, high-fat diet accelerates disuse atrophy and protein degradation in a muscle-specific manner in mice: S.L. Roseno, et al.; Nutr. Metab. 12, 39 (2015), Abstract; Full Text
Strain-Specific Interactions of Listeria monocytogenes with the Autophagy System in Host Cells: M. Cemma, et al.; PLoS One 10, e0125856 (2015), Abstract; Full Text
Activation of the ubiquitin-proteasome system against arsenic trioxide cardiotoxicity involves ubiquitin ligase Parkin for mitochondrial homeostasis: M. Watanabe, et al.; Toxicology 322, 43 (2014), Application(s): WB, IP of mouse cardiac cells, Abstract;
BAG3 induces the sequestration of proteasomal clients into cytoplasmic puncta: implications for a proteasome-to-autophagy switch: M. Minoia, et al.; Autophagy 10, 1603 (2014), Abstract; Full Text
DNA damage-specific deubiquitination regulates Rad18 functions to suppress mutagenesis: M.K. Zeman, et al.; J. Cell Biol. 206, 183 (2014), Abstract; Full Text
Nuclear proteasomes carry a constitutive posttranslational modification which derails SDS-PAGE (but not CTAB-PAGE): D.S. Pitcher, et al.; Biochim. Biophys. Acta 1844, 2222 (2014), Abstract;
p16 Protein and Gigaxonin Are Associated with the Ubiquitination of NFκB in Cisplatin-induced Senescence of Cancer Cells: M.S. Veena, et al.; J. Biol. Chem. 289, 34921 (2014), Abstract;
Phosphorylation of NBR1 by GSK3 modulates protein aggregation: N.S. Nicot, et al.; Autophagy 10, 1036 (2014), Abstract; Full Text
Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes: I. Funakoshi-Hirose, et al.; Toxicology 312, 74 (2013), Application(s): WB, IP of mouse cardiac cells, Abstract;
Autophagy inhibition promotes defective neosynthesized proteins storage in ALIS, and induces redirection toward proteasome processing and MHCI-restricted presentation: T. Wenger et al; Autophagy 8, 350 (2012), Abstract;
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme: A. Sarkar & I. Zohn; J. Cell Biol. 196, 789 (2012), Application(s): IF, WB of mouse embryonic tissue, Abstract; Full Text
Heterologous SUMO-2/3-Ubiquitin Chains Optimize IκBα Degradation and NF-κB Activity: F. Aillet, et al.; PLoS One 7, e51672 (2012), Abstract; Full Text
The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response: L.R. Butler et al; EMBO 31, 3918 (2012), Abstract;
Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62: N. Myeku, et al.; J. Biol. Chem. 286, 22426 (2011), Application(s): Western blot, Abstract; Full Text
p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different autophagy pathways: S. Mostowy, et al.; J. Biol. Chem. 286, 26987 (2011), Abstract; Full Text
Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration: S. Paine, et al.; Neurosci. Lett. 460, 205 (2009), Application(s): Immunohistochemistry of mouse hippocampus, Abstract;
Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration: K. Tamai, et al.; Am. J. Pathol. 173, 1806 (2008), Abstract;
Large-scale analysis of the human ubiquitin-related proteome : M. Matsumoto, et al.; Proteomics 5, 4145 (2005), Abstract;
Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation: D.S. Campbell et al.; Neuron 32, 1013 (2001), Abstract;
Isolation of ubiquitin-E2 (ubiquitin conjugating enzyme complexes from erythroleukaemia cells using immunoaffinity techniques: K. Takada et al.; Biochem. J. 356, 199 (2001), Abstract; Full Text
MHC Class I ubiquitination by a viral PHD/LAP finger protein: J.N. Boname et al.; Immunity 15, 627 (2001), Abstract;
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains: M. Fujimuro, et al.; Eur. J. Biochem. 249, 427 (1997), Abstract;
Serum concentrations of free ubiquitin and multiubiquitin chains: K. Takada et al.; Clin. Chem. 43, 1188 (1997), Abstract; Full Text
Immunoassay for the quantification of intracellular multi-ubiquitin chains: K. Takada, et al.; Eur. J. Biochem. 233, 42 (1995), Abstract;
Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins: M. Fujimuro, et al.; FEBS Lett. 349, 173 (1994), Abstract;

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