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Mono- and polyubiquitinylated conjugates monoclonal antibody (FK2)

Gold standard antibody for detection of ubiquitinylated proteins.
BML-PW8810-0100 100 µg 140.00 USD
BML-PW8810-0500 500 µg 559.00 USD
Do you need bulk/larger quantities?
  • Detects K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins
  • Most cited multi-ubiquitin antibody with over 500 citations
  • Validated for WB, IP, IHC, and ELISA applications
  • Available as HRP, biotin, ATTO 488, and fluorescein labeled conjugates
The hybridoma secreting the MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8805) was generated by fusion of splenocytes from Balb/c mice which had received repeated immunisation with a crude preparation of polyubiquitinylated-lysozyme. MAb to Mono- and Polyubiquitinylated Conjugates (FK2) has been extensively characterised by one-dimensional Western blotting and has been shown to recognise polyubiquitinylated and monoubiquitinylated proteins regardless of linkage location but not free ubiquitin.

Product Specification

Immunogen:Poly-ubiquitinylated lysozyme.
UniProt ID:P0CG47 (UBB), P0CG48 (UBC)
Species reactivity:Species independent
Specificity:Recognizes mono- and polyubiquitinylated conjugates.
Crossreactivity:Does not cross-react with free ubiquitin.
Applications:ELISA, IHC, IP, WB
Recommended Dilutions/Conditions:Immunoprecipitation (care must be taken as MAb to Mono- and polyubiquitinylated conjugates (FK2) demonstrates affinity for both free ubiquitin and multi-ubiquitinylated species when immobilized)
Western Blot (1:100-1:1,000)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Purity Detail:Purified.
Formulation:Liquid. In PBS containing 0.1% sodium azide.
Handling:Store unopened vial at -20°C until required for use. Store diluted antibody at 2-4°C and use within 1 month. Avoid freeze/thaw cycles.
Shipping:Shipped on Blue Ice
Long Term Storage:-20°C
Figure 1: Western blot of multi-ubiquitin chains using MAb to Polyubiquitinylated Conjugates (FK1) (Prod. No. BML-PW8805) (lanes A-C) and MAb to Mono- and Polyubiquitinylated Conjugates (FK2) (Prod. No. BML-PW8810) (lanes D-F).

Lanes A & D: K48-linked chains.Lanes B & E: K29-linked chains. Lanes C & F: K63-linked chains.

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Product Literature References

PB1 and UBA domains of p62 are essential for aggresome-like induced structure formation: M. Cabe, et al.; Biochem. Biophys. Res. Commun. 503, 2306 (2018), Abstract;
ZUFSP Deubiquitylates K63-Linked Polyubiquitin Chains to Promote Genome Stability: P. Haahr, et al.; Mol. Cell 70, 165 (2018), Abstract;
A High-Throughput Screening Strategy for Development of RNF8-Ubc13 Protein-Protein Interaction Inhibitors: E. Weber, et al.; SLAS Discov. 22, 316 (2017), Abstract;
Alternative exon skipping biases substrate preference of the deubiquitylase USP15 for mysterin/RNF213, the moyamoya disease susceptibility factor: Y. Kotani, et al.; Sci. Rep. 7, 44293 (2017), Abstract; Full Text
FKBP8 protects the heart from hemodynamic stress by preventing the accumulation of misfolded proteins and endoplasmic reticulum-associated apoptosis in mice: T. Misaka, et al.; J. Mol. Cell. Cardiol. 114, 93 (2017), Abstract;
Inverted recruitment of autophagy proteins to the Plasmodium berghei parasitophorous vacuole membrane: J. Schmuckli-Maurer, et al.; PLoS One 12, e0183797 (2017), Application(s): Indirect immunofluorescence, Abstract; Full Text
Kenny mediates selective autophagic degradation of the IKK complex to control innate immune responses: R. Tusco, et al.; Nat. Commun. 8, 1264 (2017), Abstract; Full Text
Intracellular glutathione determines bortezomib cytotoxicity in multiple myeloma cells: K.K. Starheim, et al.; Blood Cancer J. 6, e446 (2016), Application(s): Reagent, gene expression analysis, Abstract; Full Text
Mitotic phosphorylation of Bloom helicase at Thr182 is required for its proteasomal degradation and maintenance of chromosomal stability: S.S. Kharat, et al.; Oncogene 35, 125 (2016), Application(s): Immunostaining, Nu-PAGE gel, Abstract;
Npas4 transcription factor expression is regulated by calcium signalling pathways and prevents tacrolimus-induced cytotoxicity in pancreatic beta cells: T. Speckmann, et al.; J. Biol. Chem. 291, 2682 (2016), Application(s): Western blot, Abstract; Full Text
The de-ubiquitylating enzyme DUBA is essential for spermatogenesis in Drosophila: L. Koerver, et al.; Cell Death Differ. 23, 2019 (2016), Application(s): Primary antibody for western blot and for fluorescent staining, Abstract; Full Text
The Molecular Chaperone Hsp70 Promotes the Proteolytic Removal of Oxidatively Damaged Proteins by the Proteasome: S. Reeg, et al.; Free Radic. Biol. Med. 99, 153 (2016), Application(s): Immunoblot Analysis, Abstract;
Ubiquitin Ligase NEDD4 Regulates PPARγ Stability and Adipocyte Differentiation in 3T3-L1 Cells: J.J. Li, et al.; Sci. Rep. 6, 38550 (2016), Abstract; Full Text
Ubiquitination of Lysine 867 of the Human SETDB1 Protein Upregulates Its Histone H3 Lysine 9 (H3K9) Methyltransferase Activity: K. Ishimoto, et al.; PLoS One 11, e0165766 (2016), Application(s): Immunoblotting, Abstract; Full Text
USP7 is essential for maintaining Rad18 stability and DNA damage tolerance: A. Zlatanou, et al.; Oncogene 35, 965 (2016), Application(s): Western Blot, Abstract;
A point mutation in the ubiquitin ligase RNF170 that causes autosomal dominant sensory ataxia destabilizes the protein and impairs inositol 1,4,5-trisphosphate receptor-mediated Ca2+ signaling: F.A. Wright, et al.; J. Biol. Chem. 290, 13948 (2015), Abstract; Full Text
Aneuploidy causes premature differentiation of neural and intestinal stem cells: D. Gogendeau, et al.; Nat. Commun. 6, 8894 (2015), Application(s): Immunohistochemistry, Abstract;
BAF is a cytosolic DNA sensor that leads to exogenous DNA avoiding autophagy: S. Kobayashi, et al.; PNAS 112, 7027 (2015), Abstract; Full Text
Bortezomib Amplifies Effect on Intracellular Proteasomes by Changing Proteasome Structure: D.S. Pitcher, et al.; EBioMedicine 2, 642 (2015), Application(s): Western Blot, Abstract;
Effects of resveratrol on growth and skeletal muscle physiology of juvenile southern flounder: W.N. Wilson, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 183, 27 (2015), Application(s): Dot blot using fish (flounder) muscle samples, Abstract;
Histone H1 couples initiation and amplification of ubiquitin signalling after DNA damage: T. Thorslund, et al.; Nature 10, 1038 (2015), Application(s): Immunofluorescence staining and microscopy, Abstract;
Keap1 regulates inflammatory signaling in Mycobacterium avium-infected human macrophages: J.A. Awuh, et al.; PNAS 112, E4272 (2015), Abstract; Full Text
Pompe disease results in a Golgi-based glycosylation deficit in human induced pluripotent stem cell-derived cardiomyocytes: K.K. Raval, et al.; J. Biol. Chem. 290, 3121 (2015), Application(s): Western blot using human cardiomyocyte lysates, Abstract; Full Text
Activation of the ubiquitin-proteasome system against arsenic trioxide cardiotoxicity involves ubiquitin ligase Parkin for mitochondrial homeostasis: M. Watanabe, et al.; Toxicology 322, 43 (2014), Application(s): WB, IP of mouse cardiac cells, Abstract;
p16 Protein and Gigaxonin Are Associated with the Ubiquitination of NFκB in Cisplatin-induced Senescence of Cancer Cells: M.S. Veena, et al.; J. Biol. Chem. 289, 34921 (2014), Abstract;
Distinct effects of methamphetamine on autophagy-lysosome and ubiquitin-proteasome systems in HL-1 cultured mouse atrial cardiomyocytes: I. Funakoshi-Hirose, et al.; Toxicology 312, 74 (2013), Application(s): WB, IP of mouse cardiac cells, Abstract;
Autophagy inhibition promotes defective neosynthesized proteins storage in ALIS, and induces redirection toward proteasome processing and MHCI-restricted presentation: T. Wenger et al; Autophagy 8, 350 (2012), Abstract;
Hectd1 regulates intracellular localization and secretion of Hsp90 to control cellular behavior of the cranial mesenchyme: A. Sarkar & I. Zohn; J. Cell Biol. 196, 789 (2012), Application(s): IF, WB of mouse embryonic tissue, Abstract; Full Text
Heterologous SUMO-2/3-Ubiquitin Chains Optimize IκBα Degradation and NF-κB Activity: F. Aillet, et al.; PLoS One 7, e51672 (2012), Abstract; Full Text
The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response: L.R. Butler et al; EMBO 31, 3918 (2012), Abstract;
Dynamics of the degradation of ubiquitinated proteins by proteasomes and autophagy: association with sequestosome 1/p62: N. Myeku, et al.; J. Biol. Chem. 286, 22426 (2011), Application(s): Western blot, Abstract; Full Text
Immunoreactivity to Lys63-linked polyubiquitin is a feature of neurodegeneration: S. Paine, et al.; Neurosci. Lett. 460, 205 (2009), Application(s): Immunohistochemistry of mouse hippocampus, Abstract;
Loss of hrs in the central nervous system causes accumulation of ubiquitinated proteins and neurodegeneration: K. Tamai, et al.; Am. J. Pathol. 173, 1806 (2008), Abstract;
Large-scale analysis of the human ubiquitin-related proteome : M. Matsumoto, et al.; Proteomics 5, 4145 (2005), Abstract;
Chemotropic responses of retinal growth cones mediated by rapid local protein synthesis and degradation: D.S. Campbell et al.; Neuron 32, 1013 (2001), Abstract;
Isolation of ubiquitin-E2 (ubiquitin conjugating enzyme complexes from erythroleukaemia cells using immunoaffinity techniques: K. Takada et al.; Biochem. J. 356, 199 (2001), Abstract; Full Text
MHC Class I ubiquitination by a viral PHD/LAP finger protein: J.N. Boname et al.; Immunity 15, 627 (2001), Abstract;
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains: M. Fujimuro, et al.; Eur. J. Biochem. 249, 427 (1997), Abstract;
Serum concentrations of free ubiquitin and multiubiquitin chains: K. Takada et al.; Clin. Chem. 43, 1188 (1997), Abstract; Full Text
Immunoassay for the quantification of intracellular multi-ubiquitin chains: K. Takada, et al.; Eur. J. Biochem. 233, 42 (1995), Abstract;
Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins: M. Fujimuro, et al.; FEBS Lett. 349, 173 (1994), Abstract;

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