Immunocytochemistry (ICC) analysis of ubiquitinylated protein conjugates using FITC labeled mono- and polyubiquitinylated conjugates mAb (UBCJ2), (Prod. No. ENZ-ABS840F): HeLa cells, pre-treated with proteasome inhibitor MG-132 (Prod. No. BML-PI102, or ENZ-51035) at 5μM for 12 hours prior to analysis with antibody at 1:250 dilution. Panel A: isotype control (Prod. No. ADI-SAB-600FI); Panel B: untreated; Panel C: proteasome inhibitor-treated HeLa cells. Left: 20X and Right: 63X amplified.
Flow cytometry analysis of 106 Jurkat cells stained using Mono- and polyubiquitinylated conjugates mAb (UBCJ2) (FITC conjugate), (Prod. No. ENZ-ABS840F), at a concentration of 10µg/ml, side-by-side comparison to Mono- and polyubiquitinylated conjugates mAb (FK2) (fluorescein labeled), (Prod. No. BML-PW1210).
