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BIOMOL® Green

A simple and convenient method for colorimetric phosphate quantitation.
 
BML-AK111-0250 250 ml 129.00 USD
 
BML-AK111-1000 1 L 346.00 USD
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  • Convenient one-step reagent, no mixing needed
  • Extremely stable with a long shelf-life, >6 months at +4°C
  • Excellent sensitivity, ~50 pmol in 100 µl
  • Can be used in cuvette or microplate-based assays, making it ideal for high-throughput applications
  • Simple and complete kit that comes with a standardized phosphate solution for assay calibration
BIOMOL® Green provides a simple and convenient method for colorimetric phosphate quantitation (abs. 600-680 nm). Unlike other molybdate/malachite green-based assays, BIOMOL® Green doesn’t require multiple solutions or reagents prepared fresh on the day of the assay. BIOMOL® Green is extremely stable (>6 mos. at 4°C) and is simply mixed, at room temperature, with any enzymatic reaction that has released free phosphate. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.BIOMOL® Green has perhaps been most widely applied to protein phosphatase assays. Typically, a protein phosphatase (e.g. PTP1B or LAR) is simply incubated with an appropriate phosphopeptide (e.g. IR5,9,10). Addition of BIOMOL® Green stops the reaction and begins color development, which is read 20-30 min. later. BIOMOL® Green may be used in cuvette or microplate-based assays and is ideal for high-throughput applications. Each kit comes with a standardized phosphate solution for assay calibration.

Product Specification

Applications:FUNC, Colorimetric detection, HTS
 
Application Notes:Mainly used in protein phosphatase assays. Reported applications include assays for phospholipid phosphatases, tyrosyl-tRNA synthetase (coupled with pyrophosphatase) and a viral RNA triphosphatase.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Kit/Set Contains:Biomol® Green Reagent (Prod. No. BML-AK111) (1l or 250ml; liquid in screw cap plastic bottle) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
Phosphate Standard (Prod. No. BML-KI102) (1ml; 800µm phosphate in distilled water) Storage: room temperature. Long-term at 4°C; can be stored frozen without deleterious effects.
 

Product Literature References

Chemical Validation of Methionyl-tRNA Synthetase as a Druggable Target in Leishmania donovani: L.S. Torrie, et al.; ACS Infect. Dis. 3(10), 718-727 (2017), Abstract;
EYA1's conformation-specificity in dephosphorylating phosphothreonine in Myc and its activity on Myc stabilization in breast cancer: J. Li, et al.; Mol. Cell. Biol. 37, e00499-16 (2017), Abstract;
Investigation of Molecular Mechanism of Recognition between Citral and MARK4: A Newer Therapeutic Approach to Attenuate Cancer Cell Progression: F. Naz, et al.; Int. J. Biol. Macromol. (2017), Abstract;
G-rich telomeric and ribosomal DNA sequences from the fission yeast genome form stable G-quadruplex DNA structures in vitro and are unwound by the Pfh1 DNA helicase: M. Wallgren, et al.; Nucleic Acids Res. 44, 6213 (2016), Application(s): ATPase assay, Abstract; Full Text
Ischemia/reperfusion‐induced alterations of enzymatic and signaling functions of the rat cardiac Na+/K+‐ATPase: protection by ouabain preconditioning: A. Belliard, et al.; Physiol. Rep. 4, e12991 (2016), Application(s): Na+/K+‐ATPase activity, rat heart tissue homogenates, Abstract; Full Text
SERCA, complex I of the respiratory chain and ATP-synthase inhibition are involved in pleiotropic effects of NS1619 on endothelial cells: A. Lukasiak, et al. ; Eur. J. Pharmacol. 786, 137 (2016), Application(s): Ca2+-ATPase activity measurements, Abstract;
Zinc coordination is essential for the function and activity of the type II secretion ATPase EpsE: C.S. Rule, et al.; MicrobiologyOpen 5, 870 (2016), Application(s): ATPase activity assays, Abstract; Full Text
Altered cofactor regulation with disease-associated p97/VCP mutations: X. Zhang, et al.; PNAS 112, E1705 (2015), Application(s): Assay, Abstract; Full Text
Characterization of Wall Teichoic Acid Degradation by the Bacteriophage ϕ 29 Appendage Protein GP12 Using Synthetic Substrate Analogs: C.L. Myers, et al.; J. Biol. Chem. 290, 19133 (2015), Application(s): Measurement of inorganic phosphate, Abstract; Full Text
Covalent Docking Predicts Substrates for Haloalkanoate Dehalogenase Superfamily Phosphatases: N. London, et al.; Biochemistry 54, 528 (2015), Application(s): Empirical screen, Abstract; Full Text
Direct Ionic Regulation of the Activity of Myo-Inositol Biosynthesis Enzymes in Mozambique Tilapia: F.D. Villarreal, et al.; PLoS One 10, e0123212 (2015), Application(s): Colorimetric detection, Abstract; Full Text
Functional structure and physiological functions of mammalian wild-type HSP60: T. Okamoto, et al.; Arch. Biochem. Biophys. 586, 10 (2015), Application(s): ATPase activity assay, Abstract;
Membrane Topology and Biochemical Characterization of the Escherichia coli BacA Undecaprenyl-Pyrophosphate Phosphatase: G. Manat, et al. ; PLoS One 10, e0142870 (2015), Application(s): Phosphatase assay, Abstract; Full Text
Phospholemman is not required for the acute stimulation of Na+-K+-ATPase α2-activity during skeletal muscle fatigue: P. Manoharan, et al.; Am. J. Physiol. Cell Physiol. 309, C813 (2015), Abstract;
Rare Manifestation of a c.290 C>T, p.Gly97Glu VCP Mutation: N. U. Jerath, et al.; Case Rep. Genet. 2015, 239167 (2015), Application(s): Microplate, Abstract; Full Text
The catalytic role of the M2 metal ion in PP2Cα: C. Pan, et al.; Sci. Rep. 5, 8560 (2015), Abstract;
The second-sphere residue T263 is important for the function and catalytic activity of PTP1B via interaction with the WPD-loop: P. Xiao, et al.; Int. J. Biochem. Cell Biol. 57, 84 (2014), Abstract;
Protein phosphatase 4 is phosphorylated and inactivated by Cdk in response to spindle toxins and interacts with γ-tubulin: M. Voss, et al.; Cell Cycle 12, 2876 (2013), Application(s): Assay, Abstract; Full Text
Cdc14 phosphatases preferentially dephosphorylate a subset of cyclin-dependent kinase (Cdk) sites containing phosphoserine: S.C. Bremmer, et al.; J. Biol. Chem. 287, 1662 (2012), Application(s): Detection of inorganic phosphate, Abstract; Full Text
Osteoclast migration on phosphorylated osteopontin is regulated by endogenous tartrate-resistant acid phosphatase: B. Ek-Rylander, et al.; Exp. Cell Res. 316, 443 (2010), Abstract;
Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides: K. W. Harder et al.; Biochem. J. 298 , 395 (1994), Abstract;
Use of fluorinated tyrosine phosphates to probe the substrate specificity of the low molecular weight phosphatase activity of calcineurin: B. Martin et al.; J. Biol. Chem. 260, 14932 (1985), Abstract;

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