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Caspase-2 monoclonal antibody (11B4)

 
ALX-804-356-C100 100 µg 378.00 USD
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Product Specification

Clone:11B4
 
Host:Rat
 
Isotype:IgG2a
 
Immunogen:Recombinant human caspase-2 p19 fragment.
 
UniProt ID:P42575
 
Species reactivity:Human, Mouse, Rat
Dog, Monkey
 
Specificity:Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot.
 
Applications:IP, WB
 
Recommended Dilutions/Conditions:Western Blot (2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot.
For FC, ICC and IHC (FS) use MAb to Caspase-2 (10C6) (Prod. No. ALX-804-355).
 
Purity Detail:Protein G-affinity purified.
 
Formulation:Liquid. In PBS containing 0.02% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Short Term Storage:+4°C
 
Long Term Storage:-20°C
 
804-356-n2
Figures: Western blot analysis of cell lysates from human and mouse cell lines with clone 11B4. Clone 11B4 demonstrates its ability to recognize an approximately 51 kDa protein that corresponds to endogenous pro-caspase-2. Hsp-70 was used as an equal loading control, and -/- caspase-2 cells were used as a negative control. 
804-356-n1
Figure: Detection of procaspase-2 (p51), an intermediate cleavage product of caspase-2 (p32) and activated caspase-2 (p19)  in FDC-P1 cells (mouse IL-3 dependent promyelocytic line) during growth factor withdrawal induced apoptosis. Activation of caspase-2 is inhibited in the presence of the caspase inhibitor Z-VAD-FMK (ALX-260-020 or ALX-260-138 (Ready-to-Use)).
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804-356-n2 804-356-n1

Product Literature References

A Novel Cell Lysis Approach Reveals That Caspase-2 Rapidly Translocates from the Nucleus to the Cytoplasm in Response to Apoptotic Stimuli: A.A. Tinnikov, et al.; PLoS One 8, e61085 (2013), Application(s): WB using human cell lysates, Abstract; Full Text
Death of p53-defective cells triggered by forced mitotic entry in the presence of DNA damage is not uniquely dependent on Caspase-2 or the PIDDosome: C. Manzl, et al.; Cell Death Dis. 4, e942 (2013), Application(s): WB using mouse and human cell lysates, Abstract; Full Text
Determining the Contributions of Caspase-2, Caspase-8 and Effector Caspases to Intracellular VDVADase Activities during Apoptosis Initiation and Execution: M.E. Delgado, et al.; Biochim. Biophys. Acta 1833, 2279 (2013), Application(s): WB using human cell lysates, Abstract;
Maximal killing of lymphoma cells by DNA damage-inducing therapy requires not only the p53 targets Puma and Noxa, but also Bim: L. Happo, et al.; Blood 116, 5256 (2010), Abstract;
Ordering of caspases in cells undergoing apoptosis by the intrinsic pathway: S. Inoue, et al.; Cell Death Differ. 16, 1053 (2009), Application(s): WB using rat cell lysates, Abstract;
Unilateral blood flow decrease induces bilateral and symmetric responses in the immature brain: S. Villapol, et al.; Am. J. Pathol. 175, 2111 (2009), Application(s): WB using rat tissue homogenates, Abstract;
Treatment of mice with 2,3,7,8-tetrachlorodibenzo-p-dioxin leads to aryl hydrocarbon receptor-dependent nuclear translocation of NF-kappaB and expression of Fas ligand in thymic stromal cells and consequent apoptosis in T cells: I.A. Camacho, et al.; J. Immunol. 175, 90 (2005), Abstract; Full Text
BOK and NOXA Are Essential Mediators of p53-dependent Apoptosis: A.G. Yakovlev, et al.; J. Biol. Chem. 279, 28367 (2004), Abstract; Full Text
The PIDDosome, a Protein Complex Implicated in Activation of Caspase-2 in Response to Genotoxic Stress: A. Tinel & J. Tschopp; Science 304, 843 (2004), Abstract;
A novel Apaf-1-independent putative caspase-2 activation complex: S.H. Read, et al.; J. Cell Biol. 159, 739 (2002), Abstract; Full Text
Caspase-2 is not required for thymocyte or neuronal apoptosis even though cleavage of caspase-2 is dependent on both Apaf-1 and caspase-9: L.A. O'Reilly, et al.; Cell Death Differ. 9, 832 (2002), Abstract; Full Text
Requirement for caspase-2 in stress-induced apoptosis before mitochondrial permeabilization: P. Lassus, et al.; Science 297, 1352 (2002), Abstract;

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ALX-804-355-C100 100 µg 378.00 USD
Do you need bulk/larger quantities?
 

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