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Monophosphoryl Lipid A from E. coli, Serotype R515 (Re) (TLRgrade™) (Ready-to-Use)

TLR4 activator
 
ALX-581-203-R500 500 µl 263.00 USD
 
ALX-581-203-L001 1 ml 394.00 USD
Do you need bulk/larger quantities?
 

Product Specification

Alternative Name:MPL-A from E. coli, Serotype R515 (Re)
 
Source:Isolated from E. coli, Serotype R515 (Re mutant). Prepared by acid hydrolysis from purified E. coli, Serotype R515 (Re) LPS (isolated by a modification of the PCP extraction method), converted to the uniform salt form and dissolved in sterile pyrogen-free double distilled water.
 
Concentration:1mg/ml
 
Formulation:Liquid. Sterile, ready-to-use solution in pyrogen-free double distilled water.
 
Purity:≥99.9% (regarding protein or DNA contaminants; KDO not detectable)
 
Activity:Activates TLR4 (Toll-like receptor 4). Does not activate TLR2 as determined with splenocytes and macrophages from TLR4 deficient mice. Exhibits reduced endotoxic and pyrogenic activity compared to lipid A and LPS.
 
Shipping:Ambient
 
Long Term Storage:+4°C
 
Handling:Keep sterile.
 

Product Literature References

Isolation and purification of R-form lipopolysaccharides: C. Galanos & O. Luderitz; Meth. Carbohydr. Chem. 9, 11 (1993),
Electrodialysis of lipopolysaccharides and their conversion to uniform salt forms: C. Galanos & O. Luderitz; Eur. J. Biochem. 54, 603 (1975), Abstract;
A new method for the extraction of R lipopolysaccharides: C. Galanos, et al.; Eur. J. Biochem. 9, 245 (1969), Abstract;

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