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LPS from Salmonella abortus equi S-form (TLRGRADE™) (Ready-to-Use) (Biotin)

TLR4 activator
 
ALX-581-150-R500 500 µl 369.00 USD
Do you need bulk/larger quantities?
 
Useful reagent for evaluating LPS binding and cell activation in white blood cells.

Product Specification

Alternative Name:Lipopolysaccharide from Salmonella abortus equi S-form
 
Source:Smooth (S)-form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method. LPS-Biotin was prepared using the biotin reagent biotinamidocaproate N-hydroxysuccinimide ester. Briefly, LPS at 10mg/ml in distilled water was mixed with biotin reagent in sodium bicarbonate buffer. The reaction mixture was stirred, dialyzed extensively against distilled water in the dark, and sterile filtered.
 
Concentration:1mg/ml
 
Formulation:Liquid. Sterile, ready-to-use solution in pyrogen-free double-distilled water.
 
Purity:Absence of dectectable protein or DNA contaminants with agonistic TLR activity.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:+4°C
 
Handling:Do not ingest. Wear gloves and mask when handling this product! Avoid contact through all modes of exposure. LPS compounds are highly pyrogenic. Avoid accidental injection; extreme care should be taken when handling in conjunction with hypodermic syringes. Use must be restricted to qualified personnel. After opening, prepare aliquots and store at -20°C.
 
Technical Info/Product Notes:Note: As the biotinylation reagent and standard biotinylation protocol favors protein/peptide (contaminants) about 1000-fold over sugar as a target, it is mandatory to use >99.9% pure LPS to exclude the biotinylation of contaminants. These are usually present in many commercial LPS preparations and would lead to misleading observations of the tracking/staining with the reagents. TLRGRADE™ LPS fulfills such high quality requirements and the biotinylation procedure is chosen to preserve TLR4 (Toll-like receptor 4) binding and cellular activation using TLRGRADE™ LPS-Biotin.

For the unconjugated LPS, please see ALX-581-009).
 

Product Literature References

Increased TNF expression in CD43++ murine blood monocytes: B. Burke, et al.; Immunol. Lett. 118, 142 (2008), Abstract;
Influence of EDTA and heparin on lipopolysaccharide binding and cell activation, evaluated at single-cell level in whole blood: M.K. Brunialti, et al.; Cytometry 50, 14 (2002), Abstract; Full Text
Lipopolysaccharide-cell interaction and induced cellular activation in whole blood of septic patients: R. Salomao, et al.; J. Endotoxin Res. 8, 371 (2002), Abstract;
Peripheral blood mononuclear cell activation induced by Leptospira interrogans glycolipoprotein: D. Diament, et al.; Infect. Immun. 70, 1677 (2002), Abstract; Full Text
Newer aspects of the chemsitry and biology of bacterial lipopolysaccharides, with special reference to their lipid A component: C. Galanos, et al.; Int. Rev. Biochem. 14, 239 (1977),

Related Products

LPS from Salmonella abortus equi S-form (TLRGRADE™) (Ready-to-Use) 

TLR4 activator
Smooth (S)-form LPS, isolated and purified from Salmonella abortus equi by modification of the phenol water extraction and PCP method, converted to the uniform salt form and dissolved in pyrogen-free double distilled water., Absence of detectable protein or DNA contaminants with agonistic TLR activity. | Print as PDF
 
ALX-581-009-L001 1 ml 156.00 USD
 
ALX-581-009-L002 2 ml 231.00 USD
Do you need bulk/larger quantities?
 

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