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Z-YVAD-AFC

Caspase-1 substrate
 
ALX-260-035-M001 1 mg 61.00 USD
 
ALX-260-035-M005 5 mg 117.00 USD
Do you need bulk/larger quantities?
 
Replaces Prod. #: BML-P406

Fluorogenic substrate for caspase-1. AFC has an excitation maximum of 400nm and an emission maximum of 505nm. This product is similar to Ac-YVAD-AMC, Prod. No. ALX-260-024, but the AFC fluorophore has a greater Stokes' shift upon cleavage and the N-terminal is blocked by a benzyloxycarbonyl moiety, which increases cell permeability.

Product Specification

Alternative Name:Caspase-1 substrate (fluorogenic)
 
Sequence:Z-Tyr-Val-Ala-Asp-AFC (AFC = 7-Amido-4-trifluoromethylcoumarin)
 
Formula:C39H40N5O11F3
 
MW:811.8
 
CAS:201608-13-1
 
Purity:≥95% (HPLC).
Single spot (TLC)
 
Appearance:White to off-white powder.
 
Solubility:Soluble in DMSO, DMF or methanol; insoluble in water.
 
Shipping:Ambient
 
Long Term Storage:-20°C
 
Use/Stability:DMF or DMSO stock solution highly stable at -20°C.
 
Handling:Protect from light.
 

Product Literature References

Laquinimod decreases Bax expression and reduces caspase-6 activation in neurons: D. Ehrnhoefer, et al.; Exp. Neurol. 283A, 121 (2016), Application(s): Western blot, Abstract;
Activity-Based Proteomic Profiling of Deubiquitinating Enzymes in Salmonella-Infected Macrophages Leads to Identification of Putative Function of UCH-L5 in Inflammasome Regulation: E. Kummari, et al.; PLoS One 10, e0135531 (2015), Application(s): Measurement of caspase-1, Abstract; Full Text
Activation of multiple interleukin-1beta converting enzyme homologues in cytosol and nuclei of HL-60 cells during etoposide-induced apoptosis: L.M. Martins, et al.; J. Biol. Chem. 272, 7421 (1997), Abstract; Full Text
BAX-induced cell death may not require interleukin 1 beta-converting enzyme-like proteases: J. Xiang, et al.; PNAS 93, 14559 (1996), Abstract;
Crystal structure of the cysteine protease interleukin-1 beta- converting enzyme: a (p20/p10)2 homodimer: N.P. Walker, et al.; Cell 78, 343 (1994), Abstract;
Interleukin-1 beta converting enzyme: N.A. Thornberry; Meth. Enzymol. 244, 615 (1994), Abstract;
Peptidic p-nitroanilide substrates of interleukin-1 β-converting enzyme: L.A. Reiter; Int. J. Pept. Protein Res. 43, 87 (1994), Abstract;
Interleukin 1 beta (IL-1 beta) processing in murine macrophages requires a structurally conserved homologue of human IL-1 beta converting enzyme: S.M. Molineaux, et al.; PNAS 90, 1809 (1993), Abstract;
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes: N.A. Thornberry, et al.; Nature 356, 768 (1992), Abstract;
Molecular cloning of the interleukin-1 beta converting enzyme: D.P. Cerretti, et al.; Science 256, 97 (1992), Abstract;
IL-1-converting enzyme requires aspartic acid residues for processing of the IL-1 beta precursor at two distinct sites and does not cleave 31-kDa IL-1 alpha: A.D. Howard, et al.; J. Immunol. 147, 2964 (1991), Abstract;

Related Products

Ac-YVAD-AMC 

Caspase-1 substrate
≥98% (HPLC) | Print as PDF
 
ALX-260-024-M001 1 mg 43.00 USD
 
ALX-260-024-M005 5 mg 123.00 USD
Do you need bulk/larger quantities?
 

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