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Caspase-1 substrate
ALX-260-024-M001 1 mg 63.00 USD
ALX-260-024-M005 5 mg 162.00 USD
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Replaces Prod. #: BML-P401

Fluorogenic caspase-1 substrate with a Km=14µM. Ex.:340-360nm, Em.:440-460nm.Sequence is based on precursor interleukin-1β Asp116 cleavage site for caspase-1.

Product Details

Alternative Name:Caspase-1 substrate (fluorogenic)
Sequence:Ac-Tyr-Val-Ala-Asp-AMC (AMC = 7-Amino-4-methylcoumarin)
Peptide Content:75-95%
Purity:≥98% (HPLC)
Appearance:White to off-white powder.
Solubility:Soluble in DMSO. Dilute with distilled water or buffer, pH 7.0-7.5 (e. g. TRIS, HEPES).
Shipping:Ambient Temperature
Long Term Storage:-20°C
Handling:Protect from light. Keep cool and dry.
Technical Info/Product Notes:AMC has an excitation maximum of 340-360nm and an emission maximum of 440-460nm.
Regulatory Status:RUO - Research Use Only

Product Literature References

Neutrophil membrane-coated nanoparticles exhibit increased antimicrobial activities in an anti-microbial resistant K. pneumonia infection model: J. Liu, et al.; Nanomedicine 48, 102640 (2023), Abstract;
Trimethylamine N-Oxide (TMAO) Mediates Increased Inflammation and Colonization of Bladder Epithelial Cells during a Uropathogenic E. coli Infection In Vitro: R. Wu, et al.; Pathogens 12, 523 (2023), Abstract;
Cardioprotective Effects of Oroxylum indicum Extract Against Doxorubicin and Cyclophosphamide-Induced Cardiotoxicity: S.R. Pondugula, et al.; Cardiovasc. Toxicol. 22, 67 (2022), Abstract;
The structure of the human LACTB filament reveals the mechanisms of assembly and membrane binding: J.A. Bennett, et al.; PLoS Biol. 20, e3001899 (2022), Abstract;
β-hydroxybutyric acid attenuates oxidative stress and improves markers of mitochondrial function in the HT-22 hippocampal cell line: M. Majrashi, et al.; J. Integr. Neurosci. 20, 321 (2021), Abstract;
Clearance of intracellular Klebsiella pneumoniae infection using gentamicin-loaded nanoparticles: L. Jiang, et al.; J. Control Release 279, 316 (2018), Abstract;
Design and application of a fluorogenic assay for monitoring inflammatory caspase activity: R. Ranganathan, et al.; Anal. Biochem. 543, 1 (2017), Abstract;
Reperfusion therapy with recombinant human relaxin-2 (Serelaxin) attenuates myocardial infarct size and NLRP3 inflammasome following ischemia/reperfusion injury via eNOS-dependent mechanism: J.V. Raleigh, et al.; Cardiovasc Res 113, 609 (2017), Application(s): Caspase-1 activity in mouse heart homogenates, Abstract;
Temporal Changes in Caspase-1 and Caspase-8 Activities Following Brain Hypoxia With and Without Src kinase Inhibition in a Piglet Animal Model: D. Angelis, et al.; Neurochem. Res. 40, 2270 (2015), Abstract;
Crystal structure of the cysteine protease interleukin-1 β-converting enzyme: a (p20/p10)2 homodimer: N.P.C. Walker, et al.; Cell 78, 343 (1994), Abstract;
Interleukin-1 β converting enzyme: N.A. Thornberry; Meth. Enzymol. 244, 615 (1994), Abstract;
Interleukin 1 beta (IL-1 beta) processing in murine macrophages requires a structurally conserved homologue of human IL-1 beta converting enzyme: S.M. Molineaux, et al.; PNAS 90, 1809 (1993), Abstract;
A novel heterodimeric cysteine protease is required for interleukin-1 β processing in monocytes: N.A. Thornberry, et al.; Nature 356, 768 (1992), Abstract;
Molecular cloning of the interleukin-1 beta converting enzyme: D.P. Cerretti, et al.; Science 256, 97 (1992), Abstract;
IL-1-converting enzyme requires aspartic acid residues for processing of the IL-1 beta precursor at two distinct sites and does not cleave 31-kDa IL-1 alpha: A.D. Howard, et al.; J. Immunol. 147, 2964 (1991), Abstract;

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