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HSF1 (human), (recombinant) (His-tag)

 
ADI-SPP-900-D 50 µg 207.00 USD
 
ADI-SPP-900-F 200 µg 490.00 USD
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Product Specification

Alternative Name:HSTF1, Heat shock factor protein 1
 
Recommended Dilutions/Conditions:Western Blot (10ng, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
MW:~60kDa (predicted), ~70kDa (observed due to His-tag)
 
Source:Produced in E. coli. Human HSF1 is fused at the N-terminus to a His-tag.
 
UniProt ID:Q00613
 
Formulation:Liquid. In 1x TRIS buffered saline containing 5% glycerol.
 
Purity:≥90% (~60% full-length)
 
Purity Detail:Purified by multi-step chromatography.
 
Applications:WB
in vitro Assay
 
Application Notes:Western blot control.
 
Shipping:Shipped on Dry Ice
 
Long Term Storage:-80°C
 
Scientific Background:In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. The expression of heat shock proteins is mediated by the binding of a heat shock transcriptional factor (HSF) to a heat shock response element (HSE) which is located upstream of heat shock protein genes. Two heat shock transcriptional factors, termed HSF1 and HSF2, have been identified in mice and humans. In unstressed cells, HSF1 is a non-DNA binding monomeric protein present in the cytosol and nuclear compartments. However, in response to heat and heavy metals, HSF1 rapidly converts to a transcriptionally active form - a process requiring oligomerization to a trimer, serine phosphorylation, and translocation to the nucleus. HSF2, on the other hand, is not activated by heat, but is involved in regulating heat shock gene transcription during cell differentiation and development.
 
SPP-900 SDS-PAGE
SDS-PAGE analysis: Lane 1: MWM, Lane 2: 2μg HSF1.
SPP-900 WB
Western Blot analysis: Lane 1: MW marker, Lane 2: HSF1 probed with HSF1 pAb.
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SPP-900 SDS-PAGE SPP-900 WB

Product Literature References

Novel window on early human neurodevelopment via fetal exosomes in maternal blood: L. Goetzl, et al.; Ann. Clin. Transl. Neurol. 3, 381 (2016), Application(s): Quantified by ELISAs, Abstract; Full Text
In vivo binding of active heat shock transcription factor 1 to human chromosome9 heterochromatin during stress: C. Vourc'h, et al. ; J. Cell Biol. 156, 775 (2002), Application(s): In Vitro Assay , Abstract;
Role of cyclopentenone prostaglandins in rat carrageenin pleurisy: M. Di Rosa, et al. ; FEBS Lett. 508, 61 (2001), Application(s): WB , Abstract;

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Heat Shock Proteins & the Cellular Stress Response Catalog
Heat Shock Proteins & the Cellular Stress Response Catalog
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