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HSP25 polyclonal antibody

 
ADI-SPA-801-D 50 µg 161.00 USD
 
ADI-SPA-801-F 200 µg 356.00 USD
 
ADI-SPA-801-J 1 mg 1,456.00 USD
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Product Specification

Alternative Name:HspB1, Heat shock protein 25
 
Host:Rabbit
 
Immunogen:Recombinant mouse Hsp25.
 
UniProt ID:P14602
 
GenBank ID:L07577
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog, Gerbil, Guinea pig, Hamster, Porcine
 
Applications:ICC, IF, IP, WB
Electron microscopy
 
Recommended Dilutions/Conditions:Immunoprecipitation (1:100)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~25kDa by Western blot.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hsp25 is a member of the family of small heat shock proteins (sHsps), an evolutionarily conserved family of stress proteins which exhibit moleclular weights between 14-45 kDa. The sHsps are related to the alpha-crystallins, and generally exist as large oligomeric complexes that serve to stabilize and assist in refolding of denatured proteins. Hsp25 is highly homologous to the human Hsp27, and its expression is seen in many species, including mouse, rat, bovine, canine, and hamster cells.
 
ADI-SPA-801 WB
Western Blot analysis of HSP25, pAb. Lane 1: MW Marker; Lane 2: PC-12 Cell Lysate (Heat Shock), Lane 3: 3T3 Cell Lysate (Heat Shock), Lane 4: HSP25 (mouse), (recombinant) (Prod. No. ADI-SPP-510).
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ADI-SPA-801 WB

Product Literature References

Mechanical signals activate p38 MAPK pathway-dependent reinforcement of actin via mechanosensitive HspB1: L. Hoffman, et al.; Mol. Biol. Cell 28, 2661 (2017), Abstract; Full Text
Overexpression of Human SOD1 Leads to Discrete Defects in the Cerebellar Architecture in the Mouse: P. Afshar, et al.; Front. Neuroanat. 11, 22 (2017), Abstract; Full Text
Butyrate and bioactive proteolytic form of Wnt-5a regulate colonic epithelial proliferation and spatial development: K. Uchiyama, et al.; Sci. Rep. 6, 32094 (2016), Application(s): Western blot, mouse colonic epithelial YAMC cells, Abstract; Full Text
Central injection of fibroblast growth factor 1 induces sustained remission of diabetic hyperglycemia in rodents: J.M. Scarlett, et al.; Nat. Med. 22, 800 (2016), Application(s): Western blot, Abstract;
Functional effects of milk bioactive peptides on skeletal muscle of rats: C.S. Moura, at al.; Food Res. Int. 84, 18 (2016), Application(s): Western blot,
Heat Shock Protein Beta-1 Modifies Anterior to Posterior Purkinje Cell Vulnerability in a Mouse Model of Niemann-Pick Type C Disease: C. Chung, et al.; PLoS One. 12, e1006042 (2016), Application(s): Western blot, Abstract; Full Text
In vivo experimental model of orthotopic dental pulp regeneration under the influence of photobiomodulation therapy: M.S. Moreira, et al.; J. Photochem. Photobiol. B 166, 180 (2016), Abstract;
Increased Clearance of Reactive Aldehydes and Damaged Proteins in Hypertension-Induced Compensated Cardiac Hypertrophy: Impact of Exercise Training: J. C. Campos, et al.; Oxid. Med. Cell. Longev. 2015, 464195 (2015), Application(s): Western Blot, Abstract; Full Text
Linking HSP90 target occupancy to HSP70 induction and efficacy in mouse brain: K. Thirstrup, et al.; Pharmacol. Res. 104, 197 (2015), Application(s): Immunoblot analysis using mouse cell and tissue homogenates, Abstract;
Phosphorylation of Hsp25 at serine 15 is promoted in immature fibers following in situ muscle contraction: F. Kawano, et al.; J. Phys. Fitness Sports Med. 4, 231 (2015), Application(s): Western Blot, Full Text
AICAR-induced activation of AMPK negatively regulates myotube hypertrophy through the HSP72-mediated pathway in C2C12 skeletal muscle cells: T. Egawa, et al.; Am. J. Physiol. Endocrinol. Metab. 306, E344 (2014), Application(s): WB using mouse cell lysate, Abstract; Full Text
Effect of HSP25 loss on muscle contractile function and running wheel activity in young and old mice: K.A. Huey, et al.; Front. Physiol. 4, 398 (2013), Application(s): WB using muscle samples, Abstract; Full Text
Basal and stress-induced Hsp70 are modulated by ataxin-3: C.P. Reina, et al.; Cell Stress Chaperones 17, 729 (2012), Application(s): WB using mouse cell lysate, Abstract; Full Text
HSPB2 is dispensable for the cardiac hypertrophic response but reduces mitochondrial energetics following pressure overload in mice: T. Ishiwata, et al.; PLoS One 7, e42118 (2012), Application(s): WB using muscle samples, Abstract; Full Text
A novel mouse model with impaired dynein/dynactin function develops amyotrophic lateral sclerosis (ALS)-like features in motor neurons and improves lifespan in SOD1-ALS mice: D. Jaarsma, et al. ; Hum. Mol. Genet. 17, 2849 (2008), Application(s): IF using mouse tissue, Abstract;
Autoantibodies in the Autoimmune Disease Pemphigus Foliaceus Induce Blistering via p38 Mitogen-Activated Protein Kinase-Dependent Signaling in the Skin: D. Rubenstein, et al. ; Am. J. Pathol. 173, 1628 (2008), Application(s): WB using human tissue, Abstract;
Cell dynamics in the pulpal healing process following cavity preparation in rat molars: H. Ohshima, et al. ; Histochem. Cell Biol. 130, 773 (2008), Application(s): IHC, EM using rat tissue, Abstract;
Neuronal expression of constitutive heat shock proteins: implications for neurodegenerative diseases: S. Chen & I.R. Brown; Cell Stress Chaperones 12, 51 (2007), Application(s): WB, IF using rat tissue, Abstract;
Gender differences in the expression of heat shock proteins: the effect of estrogen: A.A. Knowlton, et al. ; Am. J. Physiol. Heart Circ. Physiol. 285, H687 (2003), Application(s): WB using rat samples, Abstract;
Hsp27 associates with actin and limits injury in energy depleted renal epithelia: N.J. Siegel, et al. ; J. Am. Soc. Nephrol. 13, 98 (2003), Application(s): WB using porcine samples, Abstract;
17-beta-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons: F.R. Sharp, et al. ; J. Cereb. Blood Flow Metab. 22, 183 (2002), Application(s): IHC using rat & gerbil samples, Abstract;
Abdominal temperature induces region-specific p53-independent apoptosis in the cauda epididymidis of the mouse: M. Jara, et al.; Biol. Reprod. 67, 1189 (2002), Application(s): WB using mouse tissue homogenates, Abstract; Full Text
Stress-mediated signaling in PC12 cells - the role of the small heat shock protein, Hsp27, and Akt in protecting cells from heat stress and nerve growth factor withdrawal: C. Yegappan, et al. ; J. Neurochem. 83, 452 (2002), Application(s): IP using human samples, Abstract;
Crosstalk between p38, Hsp25 and Akt in spinal motor neurons after sciatic nerve injury: D.J. Wolgemuth, et al. ; Brain Res. Mol. Brain Res. 93, 199 (2001), Application(s): ICC, IP, WB, IHC using mouse samples, Abstract;
Role of p38 MAP kinase in endothelial cell alignment induced by fluid shear stress: B.E. Sumpio, et al. ; Am. J. Physiol. Heart Circ. Physiol. 280, H189 (2001), Application(s): WB using bovine samples, Abstract;

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