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Calreticulin polyclonal antibody

 
ADI-SPA-600-D 50 µg 161.00 USD
 
ADI-SPA-600-F 200 µg 356.00 USD
 
ADI-SPA-600-J 1 mg 1,404.00 USD
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Product Specification

Alternative Name:CALR, CRT
 
Host:Rabbit
 
Immunogen:Synthetic peptide corresponding to the sequence near the C-terminus of human Calreticulin.
 
UniProt ID:P27797
 
GenBank ID:AD000092
 
Species reactivity:Human, Mouse, Rat
Bovine, Dog
 
Applications:ICC, IHC, IP, WB
 
Recommended Dilutions/Conditions:Western Blot (1:1,000, human; 1:500, mouse; ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Predicted MW of ~63kDa.
 
Purity Detail:Protein A affinity purified.
 
Formulation:Liquid. In PBS containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Calreticulin (CRT) is a multifunctional, multi-compartmental protein most abundant in the ER lumen. CRT contains the ER-retrieval sequence, KDEL, and has been best characterized as a soluble molecular chaperone of new or misfolded proteins and a Ca2+- binding protein. Both CRT and its membrane bound homolog, Calnexin (CNX) interact with proteins and glycoproteins that have monoglucosylated N-glycans. The CRT/CNX cycle promotes correct folding, inhibits aggregation of folding intermediates, blocks premature oligimerization, regulates ER degradation, and provides quality control by preventing incompletely folded glycoproteins from exiting to the Golgi complex.
 
SPA-600 WB 01
Western Blot Analysis of Calreticulin: Lane 1: MWM, Lane 2: HeLa cell lysate, Lane 3: PC-12 cell lysate. Lane 4: 3T3 cell lysate
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SPA-600 WB 01

Product Literature References

Calreticulin Translocation Aggravates Endoplasmic Reticulum Stress-associated Apoptosis during Cardiomyocyte Hypoxia/Reoxygenation: F.F. Xu, et al.; Chin. Med. J. (Engl). 128, 353 (2015), Application(s): Immunofluorescent Staining, Western Blotting, Abstract; Full Text
Cytosolically expressed PrP GPI-signal peptide interacts with mitochondria: G. Guizzunti, et al.; Comm. Integr. Biol. 8, e1036206 (2015), Full Text
NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein: M. Fausther, et al.; PLoS One 9, e98568 (2014), Application(s): WB using monkey cell lysates, Abstract; Full Text
Dominant pro-vasopressin mutants that cause diabetes insipidus form disulfide-linked fibrillar aggregates in the endoplasmic reticulum: J. Birk, et al.; J. Cell Sci. 122, 3994 (2009), Abstract; Full Text
Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor: J. Lieberman, et al. ; Cell 112, 659 (2003), Application(s): ICC, WB using human samples, Abstract;
A-kinase anchor protein 84/121 are targeted to mitochondria and mitotic spindles by overlapping amino-terminal motifs: A. Feliciello, et al. ; J. Mol. Biol. 320, 663 (2002), Application(s): ICC using mouse samples, Abstract;
Bcl-Xl affects Ca2+ homeostasis by altering expression of inositol 1,4,5-trisphosphate receptors: C.B. Thompson, et al. ; PNAS 99, 9830 (2002), Application(s): WB using mouse samples, Abstract;
Myelin proteolipid protein forms a complex with integrins and may participate in integrin receptor signaling in oligodendrocytes: W.B. Macklin, et al. ; J. Neurosci. 22, 7398 (2002), Application(s): ICC, WB using rat samples, Abstract;
Pseudoachondroplasia is caused through both intra- and extracellular pathogenic pathways: P. Maurer, et al. ; J. Clin. Invest. 110, 505 (2002), Application(s): IP using bovine samples, Abstract;
The anti-adhesive activity of thrombospondin is mediated by the N-terminal domain of cell surface calreticulin: J.E. Murphy-Ullrich, et al. ; J. Biol. Chem. 277, 37219 (2002), Application(s): ICC using bovine samples, Abstract;
Folding of hepatitis C virus E1 glycoprotein in a cell-free system: S. Abrignani, et al. ; J. Virol. 75, 11205 (2001), Application(s): IP using canine samples, Abstract;
Calnexin and calreticulin binding to human thyroperoxidase is required for its first folding step(s) but is not sufficient to promote efficient cell surface expression: J.L. Franc, et al. ; Endocrinology 141, 959 (2000), Application(s): IP using human samples, Abstract;
Genetic evidence for selective transport of opsin and arrestin by kinesin-II in mammalian photoreceptors: S.B. Goldstein, et al. ; Cell 102, 175 (2000), Application(s): IHC using mouse samples, Abstract;
ERcalcistorin/protein-disulfide isomerase acts as a calcium storage protein in the endoplasmic reticulum of a living cell. Comparison with calreticulin and calsequestrin: B. Kaminer, et al. ; J. Biol. Chem. 273, 9857 (1998), Application(s): ICC, WB using human samples, Abstract;

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