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HSP47 monoclonal antibody (M16.10A1)

 
ADI-SPA-470-D 50 µg 204.00 USD
 
ADI-SPA-470-F 200 µg 409.00 USD
 
ADI-SPA-470-J 1 mg 1,701.00 USD
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Product Specification

Alternative Name:SerpinH1, Colligin, Gp46, Heat shock protein 47, Collagen-binding protein
 
Clone:M16.10A1
 
Host:Mouse
 
Isotype:IgG2bλ
 
Immunogen:Native rat HSP47.
 
UniProt ID:P50454
 
GenBank ID:X61598
 
Source:Purified from ascites.
 
Species reactivity:Human, Mouse, Rat
Bovine, Chicken, Dog, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep
 
Applications:IHC, WB
 
Recommended Dilutions/Conditions:Immunohistochemistry (1:50)
Western Blot (1:1,000, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
 
Application Notes:Detects a band of ~47kDa by Western blot.
 
Purity Detail:Protein G affinity purified.
 
Formulation:Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
 
Handling:Avoid freeze/thaw cycles.
 
Shipping:Shipped on Blue Ice
 
Long Term Storage:-20°C
 
Scientific Background:Hsp47, also known as colligin and gp46, is an ER-resident collagen-specific stress glycoprotein involved in the synthesis and assembly of varoius collagens as a molecular chaperone. Hsp47 is a member of the serpin superfamily, but localizes to the ER via binding of its C-terminal RDEL sequence to KDEL receptors in the ER. Hsp47 binds procollagen co-translationally as it enters the ER, and dissociates prior to its secretion.
 
HSP47 monoclonal antibody (M16.10A1) Western blot
Western blot analysis of HSP47, mAb (M16.10A1) (Prod. no. ADI-SPA-470): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).
HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry
Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).
HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry
Immunohistochemistry analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).
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HSP47 monoclonal antibody (M16.10A1) Western blot HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry HSP47 monoclonal antibody (M16.10A1) Immunohistochemistry

Product Literature References

Interactome Screening Identifies the ER Luminal Chaperone Hsp47 as a Regulator of the Unfolded Protein Response Transducer IRE1α: D. Sepulveda, et al.; Mol. Cell 69, 238 (2018), Abstract;
Severe disruption and disorganization of dermal collagen fibrils in early striae gravidarum: F. Wang, et al.; Br. J. Dermatol. 178, 749 (2018), Abstract;
Therapeutic potential of tranilast for the treatment of chronic graft-versus-host disease in mice: S. Mukai, et al.; PLoS One 13, (2018), Abstract; Full Text
Cell-free Generation of COPII-coated Procollagen I Carriers: L. Yuan, et al.; Bio Protoc. 7, e2450 (2017), Abstract; Full Text
Defective signaling, osteoblastogenesis and bone remodeling in a mouse model of connexin 43 C-terminal truncation: M.C. Moorer, et al.; J. Cell Sci. 130, 531 (2017), Abstract; Full Text
Heat shock protein 47 and 65-kDa FK506-binding protein weakly but synergistically interact during collagen folding in the endoplasmic reticulum: Y. Ishikawa, et al.; J. Biol. Chem. 292, 17216 (2017), Abstract; Full Text
Proteomics analysis of differential protein expression identifies Heat shock protein 47 as a predictive marker for lymph node metastasis in patients with colorectal cancer: K. Mori, et al.; Int. J. Cancer 140, 1425 (2017), Abstract;
Immunopathologic characterization of ultrasound-defined synovitis in rheumatoid arthritis patients in clinical remission: J. Ramirez, et al.; Arthritis Res. Ther. 18, 74 (2016), Application(s): For synovial fibroblasts, Abstract; Full Text
Mutants of collagen-specific molecular chaperone Hsp47 causing osteogenesis imperfecta are structurally unstable with weak binding affinity to collagen: S. Ito, et al; Biochem. Biophys. Res. Commun. 469, 437 (2016), Application(s): Western blot, Abstract;
Wound healing profiles of hyperopic-small incision lenticule extraction (SMILE): Y.C. Liu, et al.; Sci. Rep. 6, 29802 (2016), Application(s): Histology and immunohistochemistry, rabbit cornea, Abstract; Full Text
Molecular Consequences of Defective SERPINH1/HSP47 in the Dachshund Natural Model of Osteogenesis Imperfecta: U. Lindert, et al.; J. Biol. Chem. 290, 17679 (2015), Application(s): Immunofluorescence, Western Blot, Abstract; Full Text
The Kampo Medicine Daikenchuto Inhibits Peritoneal Fibrosis in Mice: M. Kitamura, et al.; Biol. Pharm. Bull. 38, 193 (2015), Application(s): Western Blotting and Immunohistochemistry, Abstract; Full Text
Yip1A, a Novel Host Factor for the Activation of the IRE1 Pathway of the Unfolded Protein Response during Brucella Infection: Y. Taguchi, et al.; PLoS Pathog. 11, e1004747 (2015), Application(s): Immunoprecipitation, Abstract; Full Text
Bleomycin delivery by osmotic minipump: similarity to human scleroderma interstitial lung disease: R. Lee, et al.; Am. J. Physiol. Lung Cell. Mol. Physiol. 306, L736 (2014), Abstract;
Primary fibroblasts of NDUFS4-/-mice display increased ROS levels and aberrant mitochondrial morphology: F. Valsecchi, et al.; Mitochondrion 13, 436 (2013), Application(s): Immunocytochemistry using primary murine muscle cells, Abstract;
Tumor budding, myofibroblast proliferation, and fibrosis in obstructing colon carcinoma: The roles of Hsp47 and basic fibroblast growth factor: C.J. Xu, et al.; Pathol. Res. Pract. 209, 69 (2013), Application(s): Immunohistochemistry using human formalin-fixed paraffin-embedded colorectal carcinoma tissue sections, Abstract;
Novel Adenoviral Gene Delivery System Targeted Against Head and NeckCancer: D. Li, et al. ; Laryngoscope 118, 650 (2008), Application(s): Flow, IF using human & hamster tissue culture, Abstract;
Renoprotective Effect of Azelnidipine in Rats: A. Furusu, et al. ; Biol. Pharm. Bull. 31, 2237 (2008), Application(s): IHC using rat samples, Abstract;
Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy: M.M. Smith, et al.; Rheumatology 47, 1172 (2008), Application(s): IHC using sheep tissue, Abstract; Full Text
Col4a1 mutation causes endoplasmic reticulum stress and genetically modifiable ocular dysgenesis: D.B. Gould, et al.; Hum. Mol. Genet. 16, 798 (2007), Application(s): IHC using mouse tissue, Abstract; Full Text
Role of heat shock protein 47, a collagen-binding chaperone, inlacrimal gland pathology in patients with cGVHD: Y. Ogawa, et al. ; Invest. Ophthalmol. Vis. Sci. 48, 1079 (2007), Application(s): IHC, IF, WB using human tissue & cell lysates, Abstract;
Specific recognition of the collagen triple helix by chaperone HSP47. II. The HSP47-binding structural motif in collagens and related proteins: T. Koide, et al.; J. Biol. Chem. 281, 11177 (2006), Application(s): WB using recombinant GST-mouse HSP47 fusion protein, Abstract; Full Text
Specific recognition of the collagen triple helix by chaperone HSP47: minimal structural requirement and spatial molecular orientation.: T. Koide, et al.; J. Biol. Chem. 281, 3432 (2006), Application(s): WB using recombinant human and mouse HSP47, Abstract; Full Text
Proteomic Analysis of Primary Cultures of Human Adipose-derived Stem Cells: Modulation by Adipogenesis: J.P. DeLany, et al.; Mol. Cell. Proteomics 4, 731 (2005), Application(s): WB using human adipose tissue cell lysates, Abstract; Full Text
Immunohistochemical Characterization of Cells in Adult Human Patellar Tendons: F.S. Chuen, et al.; J. Histochem. Cytochem. 52, 1151 (2004), Application(s): IHC using adult human patellar tendons, Abstract; Full Text
Immunohistochemical Expression of Angiogenesis-Related Markers in Oral Squamous Cell Carcinomas With Multiple Metastatic Lymph Nodes: N.G. Nikitakis, et al.; Am. J. Clin. Pathol. 119, 574 (2003), Application(s): IHC using human oral squamous cell carcinomas (SCCs), Abstract; Full Text
Localisation and phenotypical characterisation of collagen-producing cells in TGF-beta1-induced renal interstitial fibrosis: L. Wogensen, et al. ; Histochem. Cell Biol. 119, 267 (2003), Application(s): IHC, WB using mouse samples, Abstract;
Prevalence of HSP47 antigen and autoantibodies to HSP47 in the sera of patients with mixed connective tissue disease: K. Nagata, et al. ; Biochem. Biophys. Res. Commun. 303, 413 (2003), Application(s): EIA using human samples, Abstract;
Functional Linkage between the Endoplasmic Reticulum Protein Hsp47 and Procollagen Expression in Human Vascular Smooth Muscle Cells: J.G. Pickering, et al. ; J. Biol. Chem. 277, 38571 (2002), Application(s): WB using human samples, Abstract;
Sequence-specific recognition of collagen triple helices by the collagen-specific molecular chaperone HSP47: N.J. Bulleid, et al. ; J. Biol. Chem. 277, 35007 (2002), Application(s): IP using human samples, Abstract;
HSP47 expression by smooth muscle cells is increased during arterial development and lesion formation and is inhibited by fibrillar collagen: J.G. Pickering, et al. ; Arterioscler. Thromb. Vasc. Biol. 21, 40 (2001), Application(s): WB using rat samples, Abstract;
Role of atrophic tubules in development of interstitial fibrosis in microembolism-induced renal failure in rat: A. Hishida, et al. ; Am. J. Pathol. 158, 75 (2001), Application(s): IHC using rat samples, Abstract;
Intracellular Retention of Procollagen within the Endoplasmic Reticulum Is Mediated by Prolyl 4-Hydroxylase: A.R. Walmsley, et al.; J. Biol. Chem. 274, 14884 (1999), Application(s): IP using human HT1080 cells, Abstract; Full Text

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