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Bax monoclonal antibody (4F11)

ADI-AAM-140-E 100 µg 306.00 USD
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Product Specification

Alternative Name:BCL2L4, BCL2-associated X protein
Immunogen:Recombinant human Bax.
UniProt ID:Q07812
Species reactivity:Human, Rat
Applications:IHC, IP, WB
Recommended Dilutions/Conditions:Immunohistochemistry (10µg/ml)
Immunoprecipitation (5-10µg/ml)
Western Blot (0.1-1.0µg/ml colorimetric, ECL)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Application Notes:Detects a band of ~22kDa by Western blot.
Purity Detail:Protein A affinity purified.
Formulation:Lyophilized. In PBS, pH 7.2, containing 1% sucrose. Reconstitute with 100μL of distilled water.
Shipping:Blue Ice Not Frozen
Long Term Storage:+4°C
Scientific Background:Bax is an appromimately 22 kDa pro-apoptotic protein belonging to the Bcl-2 family of proteins, which are characterized by the presence of at least one Bcl-2 homology (BH) domains. Bax normally resides in the cytoplasm, but following an apoptotic stimuli, Bax undergoes a conformational change and translocates to mitochondrial membranes where it inserts and mediates the release of cytochrome c. This activates caspase-3 and perpetuates the apoptotic cascade.
AAM-140 WB
Western blot analysis of human Raji cell lysate, probed with Bax mAb (4F11)
Immunohistochemistry analysis of paraffin-embedded section of Alzheimer’s diseased (AD) brain, stained with Bax mAb (4F11).
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AAM-140 WB AAM-140 IHC

Product Literature References

Cdk5 phosphorylates p53 and regulates its activity: V.W. Johnson, et al. ; J. Neurochem. 81, 307 (2002), Application(s): WB using rat samples, Abstract;

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