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IGF-1 (human), ELISA kit

Highly sensitive ELISA, measuring as little as 50 pg/ml of IGF-1 in just 4 hours.
 
ADI-900-150 96 wells 530.00 USD
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  • Highly sensitive, detecting as low as <50 pg/ml IGF-1
  • Broad dynamic range suitable for use with a variety of samples
  • High throughput format with results for up to 40 samples in duplicate in just 4 hours
  • Fully quantitative results that surpass semi-quantitative Western blot analysis
The IGF-1 (human), EIA kit is a colorimetric immunometric enzyme immunoassay kit with results in 4 hours. Absorbance is read at 450 nm. This kit offers ultra-sensitive measurement of as little as 50pg/ml of IGF-1.
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Product Specification

Alternative Name:Insulin-like growth factor 1
 
Sensitivity:48.5 pg/ml (range 187 - 6,000 pg/ml)
 
Assay Time:4 hours
 
Applications:ELISA, Colorimetric detection
 
Application Notes:For the quantitative determination of human IGF-1 in plasma and serum.
 
Species reactivity:Human
 
Crossreactivity:Human IGF-1 (100%), human IGF-2 (0.11%) and <0.1%: IGFBP-2, IGFBP-3, IGFBP-4, mouse IGF-1, mouse IGF-2, HGH, HGF, PDGF-BB, human insulin
 
Shipping:Blue Ice Not Frozen
 
Long Term Storage:+4°C
 
Kit/Set Contains:Microtiter plate, Conjugate, Antibody, Assay buffer 10 concentrate, Standard, Wash buffer concentrate, TMB substrate, Stop solution 2, Neutralizing reagent 2, 2N HCl
 
Scientific Background:Insulin-like growth factor-I (IGF-I) is a polypeptide hormone similar in structure to insulin. It is involved in regulation of neuronal growth and development in the central and peripheral nervous system. IGF-1 is a potent inhibitor of apoptosis in neurons, and activates the Akt signaling pathway via the IGF-1R and Insulin receptors. The IGF-1R acts as a receptor for IGF-1 and the related IGF-2, and is subject to autophosphorylation on multiple tyrosine residues that regulate receptor activation and assembly of signal transduction complexes.
 
UniProt ID:P05019
 

Product Literature References

3D patterned stem cell differentiation using thermo-responsive methylcellulose hydrogel molds: W. Lee, et al.; Sci. Rep. 6, 29408 (2016), Application(s): Preparation of drug-releasing micro particles, Abstract; Full Text
Human Cortical Neural Stem Cells Expressing Insulin-Like Growth Factor-I: A Novel Cellular Therapy for Alzheimer's Disease: L.M. McGinley, et al.; Stem Cells Transl. Med. 5, 379 (2016), Application(s): Growth factor production and signaling, Abstract; Full Text
Influence of Porcine Plasma Supplementation on Gestating Sow Serum IGF-1 Concentration and Litter Weights: M. Vaughn, et al.; PAWJ (2016), Full Text
Changes in maternal body composition and metabolism of dairy goats during pregnancy: D. S. Castagnino, et al.; R. Bras. Zootec. 44, 92 (2015), Application(s): ELISA using goat plasma, Full Text
Contents of Breast Milk Obtained from Mothers of Preterm and Term Newborn Infants: D. Terek, et al.; JPR 2, 183 (2015), Application(s): Milk IGF-1 levels in the mother’s breast milk, Full Text
Association Between Omentin, Visfatin and Insulin-Like Growth Factor-1 in Women With Metabolic Syndrome: M.T. Goodarzi, et al.; Avicenna J. Med. Biochem. 2, e25184 (2014), Application(s): ELISA, Full Text
Directed axonal outgrowth using a propagating gradient of IGF‐1: W. Lee, et al.; Adv. Mater. 26, 4936 (2014), Abstract;
Heavy resistance training and peri-exercise ingestion of a multi-ingredient ergogenic nutritional supplement in males: effects on body composition, muscle performance and markers of muscle protein synthesis: M. Spillane, et al.; J. Sports. Sci. Med. 13, 894 (2014), Application(s): Quantification of IGF-1 in serum samples, Abstract; Full Text
The effect of bovine colostrum supplementation in older adults during resistance training: W.R. Duff, et al.; Int. J. Sport Nutr. Exerc. Metab. 24, 276 (2014), Abstract;
Effects of velvet antler with blood on bone in ovariectomized rats: S.H. Tseng, et al.; Molecules 17, 10574 (2012), Application(s): ELISA using ether extract of velvet antler, Abstract; Full Text
Effects of 28 days of resistance exercise while consuming commercially available pre- and post-workout supplements, NO-Shotgun and NO-Synthesize: M. Spillane, et al.; Nutr. Metab. (Lond.) 8, 78 (2011), Application(s): ELISA using human serum, Abstract; Full Text

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