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United States 

IGF-I (human), (recombinant)

ADI-908-059-0100 100 µg 256.00 USD
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Product Details

Alternative Name:Insulin-like growth factor I
Source:Produced in E. coli.
UniProt ID:P05019
Formulation:Lyophilized from a sterile filtered concentrated solution in PBS, pH 7.0.
Purity:≥95% (SDS and HPLC)
Purity Detail:Purified by SDS and HPLC.
Biological Activity:The activity as determined by the dose-dependent proliferation of mouse FDC-P1 is less than 1.0 ng/ml, corresponding to a specific activity of 1x106 units/mg.
Applications:ELISA, WB
Activity assay
Application Notes:Western blot control.
Reconstitution:Reconstitute the lyophilized IGF-1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Shipping:Dry Ice
Long Term Storage:-20°C
Use/Stability:Lyophilized IGF-I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IGF-I should be stored at +4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Handling:Avoid freeze-thaw cycles.
Scientific Background:Insulin-like growth factor-1 (IGF-1) was originally known as Somatomedin C but was later changed to IGF-1 due to its structural similarity to proinsulin. IGF-1 has insulinlike effects, but it appears to be involved in many other functions and has been linked to aging, nutrition, physical activity and cancer. While each of these is very interesting on its own, together they may shed light on the role that lifestyle plays in influencing cancer risk. High levels of circulating IGF-1 and low levels of IGF binding protein-3 (IGFBP-3) are associated with increased risk of several cancers. IGF-1 can stimulate cell proliferation and inhibit apoptosis in many differe nt kinds of cells. IGF-1 circulates in blood bound with IGFBPs. Six IGFBPs have been identified but the majority of circulating IGF -1 is complexed with IGFBP-3. The IGF-1 and IGFBP-3 exist in a complex with a third protein, named the acid -labile subunit.
Regulatory Status:RUO - Research Use Only

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