| Alternative Name: | Cell division cycle 25B, Dual specificity phosphatase CDC25B |
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| MW: | 65 kDa |
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| Source: | Produced in E. coli. Full length human CDC25B. |
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| UniProt ID: | P30305 |
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| Gene/Protein Identifier: | NM_021873 (RefSeq) |
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| Formulation: | Liquid. In 50mM TRIS/HCl, pH 8.0, 150mM NaCl, 5mM DTT, 0.1mM EDTA, 0.03% Brij 35, 20% glycerol. |
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| Purity Detail: | Partially purified by single-step affinity chromatography and gel filtration. |
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| Specific Activity: | ≥10 U/mg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH 8.2, 30°C. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute. |
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| Application Notes: | Useful for kinetic studies and high throughput screening. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Scientific Background: | CDC25 protein tyrosine phosphatases activate cyclin dependent kinases by dephosphorylating them, promoting cell division. All three of the genes encoding the CDC25 phosphatases are considered potential oncogenes and the enzymes are drug discovery targets. In mammalian cells, the CDC25B isoform activates cyclin A- and cyclin B1-containing complexes and is necessary for entry into mitosis. CDC25B -/- female mice are sterile because their oocytes are in permanent meiotic arrest resulting from the inability to activate CDK11. CDC25B shuttles between the nucleus and the cytoplasm. The protein is in the nucleus during M and G1 and moves to the cytoplasm during S and G22. |
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| Regulatory Status: | RUO - Research Use Only |
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