Ubiquitin-AMC is a fluorogenic substrate for a wide range of deubiquitinylating enzymes (DUBs), including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin specific proteases (USPs). It is a particularly useful reagent for the study of deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential. Ubiquitin-AMC has been shown to be a sensitive substrate for UCH-L3 (Prod. No. BML-UW9745) and for Isopeptidase-T (Prod. No. BML-UW9690), and is particularly useful for studying deubiquitinylating activity where detection sensitivity or continuous monitoring of activity is essential.
Product Details
| Source: | Produced in E.coli. |
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| UniProt ID: | P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52) |
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| Formulation: | In DMSO |
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| Purity: | ≥95% (HPLC) |
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| Purity Detail: | Please note: considerable efforts are taken to ensure that ubiquitin-AMC is homogeneous and not contaminated with ubiquitin and ubiquitin deletions, which are common in material obtained from other sources. The chromatogram below demonstrates the homogeneity of BML-SE211. |
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| Application Notes: | Can be used for the following applications:
1. Substrate for deubiquitinylating enzyme activity assays.
2. Identification/confirmation of enzyme deubiquitinylation activity.
3. Investigation of deconjugating enzyme substrate specificity in comparison with alternative UBL substrates. |
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| Shipping: | Dry Ice |
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| Long Term Storage: | -80°C |
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| Handling: | Avoid freeze/thaw cycles. |
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| Regulatory Status: | RUO - Research Use Only |
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Figure: HPLC analysis. Comparison of Prod. No. BML-SE-211Prod. No. BML-SE-211) (A) with contaminated alternatively sourced material (B).
Figure: DUB Assay: 50nM UCLH1, His6-tagged (UW9740); 500nM Ubiquitin-AMC; 50mM HEPES pH7.8, 0.5mM EDTA, 1mM DTT.
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Product Literature References
Agouti Related Peptide Secreted Via Human Mesenchymal Stem Cells Upregulates Proteasome Activity in an Alzheimer's Disease Model: N.K. Lee, et al.; Sci. Rep.
7, 39340 (2017),
Abstract;
Full Text
Evaluation of polyphenols from Broussonetia papyrifera as coronavirus protease inhibitors: J.Y. Park, et al.; J. Enzyme Inhib. Med. Chem.
32, 504 (2017),
Application(s): SARS-CoV deubiquitination assay,
Abstract;
Full Text
Ubiquitin Carboxy-Terminal HydrolaseL3 Correlates with Human Sperm Count, Motility and Fertilization: M. Wang, et al.; PLoS One
11, e0165198 (2016),
Application(s): Determination of UCH enzymatic activity of spermatozoa ,
Abstract;
Full Text
Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry: D. E. Mason et al.; Biochemistry
43, 6535 (2004),
Abstract;
Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes: L. C. Dang et al.; Biochemistry
37, 1868 (1998),
Abstract;
