Product Details
Alternative Name: | Microtubule-associated protein 1 light chain 3, MAP1LC3 |
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Clone: | 5H3 |
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Host: | Mouse |
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Isotype: | IgG1 |
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Immunogen: | Synthetic peptide corresponding to an internal sequence identical in LC3A, LC3B and LC3C conjugated to hemocyanin. |
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UniProt ID: | Q9H492 (LC3A), Q9GZQ8 (LC3B), Q9BXW4 (LC3C) |
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Species reactivity: | Human
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Specificity: | Recognizes both forms of endogenous LC3. |
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Applications: | ICC, IHC, WB
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Recommended Dilutions/Conditions: | Immunocytochemistry (paraformaldehyde/methanol fixation; 1-10 µg/ml)
Western Blot (0.5µg/ml for HRPO/ECL detection; Recommended blocking buffer CPPT: 10mM TRIS-HCl, pH 7.4, 0.5% (w/v) casein, 1% (w/v) PEG 4,000, 1% (w/v) polyvinylpyrrolidone, 0.1% (v/v) Tween 20, 150mM sodium chloride)
We strongly recommend to use PVDF membranes for immunoblot assays.
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application. |
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Application Notes: | Detects a band of ~18kDa (LC3-I; cytoplasmic form) and a band of ~16kDa (LC3-II; lipidated form) by Western blot. |
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Positive Control: | Included. (Prod. No. ALX-840-038) |
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Purity Detail: | Thiophilic adsorption and size exclusion chromatography purified. |
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Formulation: | Liquid. In PBS containing 50% glycerol, 0.09% sodium azide, PEG and sucrose. |
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Use/Stability: | Stable at -20°C up to 1 year. |
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Handling: | Thawed aliquots may be stored at 4°C up to 3 months. Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C. |
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Shipping: | Blue Ice |
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Long Term Storage: | -20°C |
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Scientific Background: | Autophagy is an alternative process of proteasomal degradation for some long-lived proteins or organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). Genetic studies in yeast have shown that Autophagy-defective Gene-8 (Atg-8) represents a specific marker for autophagy. Among the four families of mammalian Atg8-related proteins only LC3 (Microtubule-associated Protein1 Light Chain 3) is expressed at sufficient high levels and efficiently recruited to autophagic vesicles in cells and tissues. During autophagy the cytoplasmic form, LC3-I is processed and recruited to autophagosomes, where LC3-II is generated by site specific proteolysis near to the C-terminus. Autophagic vacuoles have been also reported frequently in cardiomyopathies or muscle cells exposed to different experimental settings. |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
DRAM1 Promotes Lysosomal Delivery of Mycobacterium marinum in Macrophages: A.B. Karp, et al.; Cells
12, 828 (2023),
Abstract;
SERTAD1 Sensitizes Breast Cancer Cells to Doxorubicin and Promotes Lysosomal Protein Biosynthesis: H.A. Nguyen, et al.; Biomedicines
10, 1148 (2022),
Abstract;
Conidiobolus coronatus induces oxidative stress and autophagy response in Galleria mellonella larvae: M. Kazek, et al.; PLoS One
15, e0228407 (2020),
Abstract;
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