Product Details
Alternative Name: | Multidrug resistance-associated protein 2, cMOAT, ABCC2 |
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Clone: | M2III-5 |
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Host: | Mouse |
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Isotype: | IgG2b |
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Immunogen: | Recombinant C-terminal rat MRP2 (multidrug resistance-associated protein 2; cMOAT) (aa 1339-1541). |
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UniProt ID: | Q63120 |
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Species reactivity: | Human, Mouse, Rat
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Specificity: | Recognizes an internal epitope of MRP2. |
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Crossreactivity: | Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP3 or MRP5. |
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Applications: | ICC, IHC (FS), IHC (PS), WB
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Recommended Dilutions/Conditions: | Immunocytochemistry: Use 1:20-1:50 dilution on acetone fixed cytospin preparations. Immunohistochemistry (frozen sections, paraffin sections): Use 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex or on paraffin sections after pretreatment with 0.01 M citric acid, pH 6.0 in distilled water at 100°C for 5 min 3 times. After washing, slides can be incubated with biotinylated rabbit anti-mouse IgG and streptavidin conjugated to horseradish peroxidase. Western Blot: Use 1:20-1:50 dilution and anti-mouse-HRP. Suggested dilutions/conditions may not be available for all applications. Optimal conditions must be determined individually for each application. |
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Application Notes: | Immunocytochemistry: cytospin preparations Immunohistochemistry: Does not recognize mouse MRP2 in IHC. |
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Formulation: | Liquid. In serum free culture supernatant containing 0.7% BSA and 0.1% sodium azide. |
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Shipping: | Blue Ice |
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Short Term Storage: | +4°C |
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Long Term Storage: | -20°C |
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Regulatory Status: | RUO - Research Use Only |
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Product Literature References
Boldine enhances bile production in rats via osmotic and Farnesoid X receptor dependent mechanisms: J. Cermanova, et al.; Toxicol. Appl. Pharmacol.
285, 12 (2015),
Application(s): Western Blotting, Immunohistochemistry,
Abstract;
Maternal bile acid transporter deficiency promotes neonatal demise: Y. Zhang, et al.; Nat. Commun.
6, 8186 (2015),
Application(s): Immunoblot analysis of tissue homogenates,
Abstract;
Analysis of the in vivo functions of Mrp3: M.G. Belinsky, et al.; Mol. Pharmacol.
68, 160 (2005),
Abstract;
Specific detection of multidrug resistance proteins MRP1, MRP2, MRP3, MRP5, and MDR3 P-glycoprotein with a panel of monoclonal antibodies: G.L. Scheffer, et al.; Cancer Res.
60, 5269 (2000),
Abstract;
Full Text
Congenital jaundice in rats with a mutation in a multidrug resistance-associated protein gene: C.C. Paulusma, et al.; Science
271, 1126 (1996),
Abstract;
General Literature References
Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines: M. Kool, et al.; Cancer Res.
57, 3537 (1997),
Abstract;